Construct: ORF TRCN0000474735
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008747.1_s317c1
- Derived from:
- ccsbBroadEn_10460
- DNA Barcode:
- ACCGCAGTCGGGCCAGTCTGCTCG
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GRAMD1C (54762)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474735
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54762 | GRAMD1C | GRAM domain containing 1C | NM_017577.5 | 99.8% | 99.6% | 1931T>C;1982delA |
| 2 | human | 54762 | GRAMD1C | GRAM domain containing 1C | XM_011512930.1 | 98.3% | 98.1% | 0_1ins30;1901T>C;1952delA |
| 3 | human | 54762 | GRAMD1C | GRAM domain containing 1C | XM_005247546.2 | 95% | 94.8% | 363_364ins96;1835T>C;1886delA |
| 4 | human | 54762 | GRAMD1C | GRAM domain containing 1C | XM_005247547.2 | 90.9% | 90.7% | 1455_1456ins177;1754T>C;1805delA |
| 5 | human | 54762 | GRAMD1C | GRAM domain containing 1C | XM_017006646.1 | 86.1% | 85.9% | (many diffs) |
| 6 | human | 54762 | GRAMD1C | GRAM domain containing 1C | NM_001172105.2 | 68.9% | 68.7% | 0_1ins615;1316T>C;1367delA |
| 7 | human | 54762 | GRAMD1C | GRAM domain containing 1C | XM_011512931.1 | 68.9% | 68.7% | 0_1ins615;1316T>C;1367delA |
| 8 | human | 54762 | GRAMD1C | GRAM domain containing 1C | XM_017006647.1 | 52.2% | 52.6% | (many diffs) |
| 9 | human | 54762 | GRAMD1C | GRAM domain containing 1C | XM_017006648.1 | 52.2% | 52.6% | (many diffs) |
| 10 | human | 54762 | GRAMD1C | GRAM domain containing 1C | XM_017006649.1 | 52.2% | 52.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2052
- ORF length:
- 1986
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gggcgctccg actgtccgtc aggtgatgaa tgaaggggat tcaagccttg 121 ccaccgactt acaggaagat gtagaggaaa atcctagtcc aactgtggaa gagaataatg 181 tggtagttaa aaaacagggg ccaaatttac ataattggag tggtgactgg agcttttgga 241 tttcaagttc cacctataaa gacaggaatg aggaatacag aagacagttc acacatctac 301 ctgatacaga gaggctgata gcagattatg cttgtgctct tcagagggac attttgcttc 361 agggacgact atacctttca gaaaactggc tatgtttcta tagcaacatc ttcagatggg 421 aaactacaat ttctattgct ttaaagaata taaccttcat gaccaaggaa aaaactgctc 481 gactcatccc aaacgctatc cagatagtta cagaaagtga aaagtttttc ttcacatctt 541 ttggtgccag ggatagaagt tacctcagta tctttaggtt gtggcagaat gtattattag 601 ataagagcct gactagacag gaattctggc aactgctcca gcagaactat ggcactgagc 661 taggtttaaa tgctgaggag atggaaaact tgtcactgtc gattgaggat gtgcagccaa 721 gaagtccagg aagaagcagc ttggatgact ctggggagag agatgaaaaa ttatccaagt 781 caatcagttt taccagtgaa tcaattagtc gggtttcaga aacagagtca ttcgatggaa 841 attcatcaaa aggaggatta ggcaaagagg agtcccaaaa tgagaaacag accaaaaaga 901 gtctcttacc aactttggaa aagaagttaa ctagagtgcc atcaaagtca ctggacttga 961 ataaaaatga atatctttct ctggacaaaa gcagcacttc agattctgtt gatgaagaaa 1021 atgttcctga gaaagatctt catggaagac tttttatcaa ccgtattttt catatcagtg 1081 ctgacagaat gtttgaattg ctctttacca gttcacgctt tatgcagaaa tttgccagtt 1141 ctagaaatat aatagatgta gtatctaccc cttggactgc agaacttgga ggtgatcagc 1201 tgagaacgat gacctacact atagtcctta atagtccact tactggaaaa tgcactgctg 1261 ccactgaaaa gcagacactg tataaagaaa gtcgggaagc acgattttat ttggtagatt 1321 cagaagtact gacacatgat gtcccctacc atgattactt ctataccgtg aacagatact 1381 gtatcatccg atcttcaaaa cagaaatgca ggctaagagt ttccacagat ttgaaataca 1441 gaaaacagcc atggggcctt gtcaaatctt taattgaaaa gaattcctgg agttctttgg 1501 aggactattt caaacagctt gaatcagatt tgttaattga agaatctgta ttaaatcagg 1561 ccattgaaga ccctggaaaa cttactggcc tacgaaggag aaggcgaacc ttcaaccgaa 1621 cagcagaaac agttcctaaa ctttcctctc agcattcctc tGGAGATGTG GGCTTAGGTG 1681 CCAAAGGGGA TATTACAGGA AAGAAAAAGG AAATGGAAAA CTATAACGTC ACTCTTATTG 1741 TGGTAATGAG TATTTTTGTG TTGTTATTAG TTTTGTTGAA TGTGACACTG TTTCTGAAGC 1801 TGTCAAAGAT AGAACATGCT GCTCAGTCCT TTTACCGTCT CCGCCTCCAA GAAGAGAAAT 1861 CTTTAAATTT AGCCTCTGAT ATGGTGTCAA GAGCAGAAAC TATTCAGAAG AATAAAGATC 1921 AGGCCCATCG TTTAAAGGGA GTGCTCCGAG ACTCCATAGT GATGCTTGAA CAGCTGAAGA 1981 GCTCACTCAT TATGCCTCAG AAAACGTTTG ATCTACTAAA TAAGAATAAG ACTGGCATGG 2041 CTGTTGAAGC TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA 2101 CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC 2161 GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAACCG CAGTCGGGCC AGTCTGCTCG 2221 ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt