Construct: ORF TRCN0000474735

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008747.1_s317c1
Derived from:
ccsbBroadEn_10460
DNA Barcode:
ACCGCAGTCGGGCCAGTCTGCTCG
Epitope Tag:
V5 (not translated due to frame shift)
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GRAMD1C (54762)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000474735
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 54762 GRAMD1C GRAM domain containing 1C NM_017577.5 99.8% 99.6% 1931T>C;1982delA
2 human 54762 GRAMD1C GRAM domain containing 1C XM_011512930.1 98.3% 98.1% 0_1ins30;1901T>C;1952delA
3 human 54762 GRAMD1C GRAM domain containing 1C XM_005247546.2 95% 94.8% 363_364ins96;1835T>C;1886delA
4 human 54762 GRAMD1C GRAM domain containing 1C XM_005247547.2 90.9% 90.7% 1455_1456ins177;1754T>C;1805delA
5 human 54762 GRAMD1C GRAM domain containing 1C XM_017006646.1 86.1% 85.9% (many diffs)
6 human 54762 GRAMD1C GRAM domain containing 1C NM_001172105.2 68.9% 68.7% 0_1ins615;1316T>C;1367delA
7 human 54762 GRAMD1C GRAM domain containing 1C XM_011512931.1 68.9% 68.7% 0_1ins615;1316T>C;1367delA
8 human 54762 GRAMD1C GRAM domain containing 1C XM_017006647.1 52.2% 52.6% (many diffs)
9 human 54762 GRAMD1C GRAM domain containing 1C XM_017006648.1 52.2% 52.6% (many diffs)
10 human 54762 GRAMD1C GRAM domain containing 1C XM_017006649.1 52.2% 52.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2052
ORF length:
1986
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga gggcgctccg actgtccgtc aggtgatgaa tgaaggggat tcaagccttg
121ccaccgactt acaggaagat gtagaggaaa atcctagtcc aactgtggaa gagaataatg
181tggtagttaa aaaacagggg ccaaatttac ataattggag tggtgactgg agcttttgga
241tttcaagttc cacctataaa gacaggaatg aggaatacag aagacagttc acacatctac
301ctgatacaga gaggctgata gcagattatg cttgtgctct tcagagggac attttgcttc
361agggacgact atacctttca gaaaactggc tatgtttcta tagcaacatc ttcagatggg
421aaactacaat ttctattgct ttaaagaata taaccttcat gaccaaggaa aaaactgctc
481gactcatccc aaacgctatc cagatagtta cagaaagtga aaagtttttc ttcacatctt
541ttggtgccag ggatagaagt tacctcagta tctttaggtt gtggcagaat gtattattag
601ataagagcct gactagacag gaattctggc aactgctcca gcagaactat ggcactgagc
661taggtttaaa tgctgaggag atggaaaact tgtcactgtc gattgaggat gtgcagccaa
721gaagtccagg aagaagcagc ttggatgact ctggggagag agatgaaaaa ttatccaagt
781caatcagttt taccagtgaa tcaattagtc gggtttcaga aacagagtca ttcgatggaa
841attcatcaaa aggaggatta ggcaaagagg agtcccaaaa tgagaaacag accaaaaaga
901gtctcttacc aactttggaa aagaagttaa ctagagtgcc atcaaagtca ctggacttga
961ataaaaatga atatctttct ctggacaaaa gcagcacttc agattctgtt gatgaagaaa
1021atgttcctga gaaagatctt catggaagac tttttatcaa ccgtattttt catatcagtg
1081ctgacagaat gtttgaattg ctctttacca gttcacgctt tatgcagaaa tttgccagtt
1141ctagaaatat aatagatgta gtatctaccc cttggactgc agaacttgga ggtgatcagc
1201tgagaacgat gacctacact atagtcctta atagtccact tactggaaaa tgcactgctg
1261ccactgaaaa gcagacactg tataaagaaa gtcgggaagc acgattttat ttggtagatt
1321cagaagtact gacacatgat gtcccctacc atgattactt ctataccgtg aacagatact
1381gtatcatccg atcttcaaaa cagaaatgca ggctaagagt ttccacagat ttgaaataca
1441gaaaacagcc atggggcctt gtcaaatctt taattgaaaa gaattcctgg agttctttgg
1501aggactattt caaacagctt gaatcagatt tgttaattga agaatctgta ttaaatcagg
1561ccattgaaga ccctggaaaa cttactggcc tacgaaggag aaggcgaacc ttcaaccgaa
1621cagcagaaac agttcctaaa ctttcctctc agcattcctc tGGAGATGTG GGCTTAGGTG
1681CCAAAGGGGA TATTACAGGA AAGAAAAAGG AAATGGAAAA CTATAACGTC ACTCTTATTG
1741TGGTAATGAG TATTTTTGTG TTGTTATTAG TTTTGTTGAA TGTGACACTG TTTCTGAAGC
1801TGTCAAAGAT AGAACATGCT GCTCAGTCCT TTTACCGTCT CCGCCTCCAA GAAGAGAAAT
1861CTTTAAATTT AGCCTCTGAT ATGGTGTCAA GAGCAGAAAC TATTCAGAAG AATAAAGATC
1921AGGCCCATCG TTTAAAGGGA GTGCTCCGAG ACTCCATAGT GATGCTTGAA CAGCTGAAGA
1981GCTCACTCAT TATGCCTCAG AAAACGTTTG ATCTACTAAA TAAGAATAAG ACTGGCATGG
2041CTGTTGAAGC TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA
2101CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC
2161GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAACCG CAGTCGGGCC AGTCTGCTCG
2221ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)