Construct: ORF TRCN0000474740
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011945.1_s317c1
- Derived from:
- ccsbBroadEn_05815
- DNA Barcode:
- GGAAGCTCTCATTGTGTGTAAGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ANGPT2 (285)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474740
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 285 | ANGPT2 | angiopoietin 2 | NM_001147.3 | 99.8% | 100% | 735G>A;1107T>C |
| 2 | human | 285 | ANGPT2 | angiopoietin 2 | NM_001118887.2 | 99.6% | 99.7% | 735G>A;799_800insCAG;1104T>C |
| 3 | human | 285 | ANGPT2 | angiopoietin 2 | NM_001118888.2 | 89.3% | 89.5% | 288_289ins156;579G>A;951T>C |
| 4 | human | 285 | ANGPT2 | angiopoietin 2 | XM_017013318.1 | 89.1% | 89.3% | (many diffs) |
| 5 | mouse | 11601 | Angpt2 | angiopoietin 2 | NM_007426.4 | 80.5% | 85.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1557
- ORF length:
- 1488
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtggcagatt gttttcttta ctctgagctg tgatcttgtc ttggccgcag 121 cctataacaa ctttcggaag agcatggaca gcataggaaa gaagcaatat caggtccagc 181 atgggtcctg cagctacact ttcctcctgc cagagatgga caactgccgc tcttcctcca 241 gcccctacgt gtccaatgct gtgcagaggg acgcgccgct cgaatacgat gactcggtgc 301 agaggctgca agtgctggag aacatcatgg aaaacaacac tcagtggcta atgaagcttg 361 agaattatat ccaggacaac atgaagaaag aaatggtaga gatacagcag aatgcagtac 421 agaaccagac ggctgtgatg atagaaatag ggacaaacct gttgaaccaa acagcggagc 481 aaacgcggaa gttaactgat gtggaagccc aagtattaaa tcagaccacg agacttgaac 541 ttcagctctt ggaacactcc ctctcgacaa acaaattgga aaaacagatt ttggaccaga 601 ccagtgaaat aaacaaattg caagataaga acagtttcct agaaaagaag gtgctagcta 661 tggaagacaa gcacatcatc caactacagt caataaaaga agagaaagat cagctacagg 721 tgttagtatc caagcaaaat tccatcattg aagaactaga aaaaaaaata gtgactgcca 781 cggtgaataa ttcagttctt caaaagcagc aacatgatct catggagaca gttaataact 841 tactgactat gatgtccaca tcaaactcag ctaaggaccc cactgttgct aaagaagaac 901 aaatcagctt cagagactgt gctgaagtat tcaaatcagg acacaccacg aatggcatct 961 acacgttaac attccctaat tctacagaag agatcaaggc cTACTGTGAC ATGGAAGCTG 1021 GAGGAGGCGG GTGGACAATT ATTCAGCGAC GTGAGGATGG CAGCGTTGAT TTTCAGAGGA 1081 CTTGGAAAGA ATATAAAGTG GGATTTGGTA ACCCTTCAGG AGAATATTGG CTGGGAAATG 1141 AGTTTGTTTC GCAACTGACT AATCAGCAAC GCTACGTGCT TAAAATACAC CTTAAAGACT 1201 GGGAAGGGAA TGAGGCTTAC TCATTGTATG AACATTTCTA TCTCTCAAGT GAAGAACTCA 1261 ATTATAGGAT TCACCTTAAA GGACTTACAG GGACAGCCGG CAAAATAAGC AGCATCAGCC 1321 AACCAGGAAA TGATTTTAGC ACAAAGGATG GAGACAACGA CAAATGTATT TGCAAATGTT 1381 CACAAATGCT AACAGGAGGC TGGTGGTTTG ATGCATGTGG TCCTTCCAAC TTGAACGGAA 1441 TGTACTATCC ACAGAGGCAG AACACAAATA AGTTCAACGG CATTAAATGG TACTACTGGA 1501 AAGGCTCAGG CTATTCGCTC AAGGCCACAA CCATGATGAT CCGACCAGCA GATTTCTTGC 1561 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1621 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1681 ATATATCTTG TGGAAAGGAC GAGGAAGCTC TCATTGTGTG TAAGCCACGC GTTAAGTCga 1741 caatcaacct ctggattaca aaatttgtga aagatt