Transcript: Human XM_017013318.1

PREDICTED: Homo sapiens angiopoietin 2 (ANGPT2), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ANGPT2 (285)
Length:
4851
CDS:
487..1818

Additional Resources:

NCBI RefSeq record:
XM_017013318.1
NBCI Gene record:
ANGPT2 (285)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017013318.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000059226 GCCGCAGCCTATAACAACTTT pLKO.1 532 CDS 100% 5.625 3.938 N ANGPT2 n/a
2 TRCN0000059223 CCCTAATTCTACAGAAGAGAT pLKO.1 1233 CDS 100% 4.950 3.465 N ANGPT2 n/a
3 TRCN0000059224 GCCACGGTGAATAATTCAGTT pLKO.1 1039 CDS 100% 4.950 3.465 N ANGPT2 n/a
4 TRCN0000059225 GCTTACTCATTGTATGAACAT pLKO.1 1474 CDS 100% 4.950 3.465 N ANGPT2 n/a
5 TRCN0000166201 CATGGTGAAACCCTGTCTCTA pLKO.1 3160 3UTR 100% 4.950 2.475 Y ORAI2 n/a
6 TRCN0000179120 CAACATGGTGAAACCCTGTTT pLKO.1 3157 3UTR 100% 4.950 2.475 Y LOC339059 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017013318.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05815 pDONR223 100% 89.1% 89.3% None (many diffs) n/a
2 ccsbBroad304_05815 pLX_304 0% 89.1% 89.3% V5 (many diffs) n/a
3 TRCN0000474740 GGAAGCTCTCATTGTGTGTAAGCC pLX_317 37.4% 89.1% 89.3% V5 (many diffs) n/a
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