Construct: ORF TRCN0000474741
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009178.1_s317c1
- Derived from:
- ccsbBroadEn_03718
- DNA Barcode:
- TACACGTCCATCCGCTTCTACACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IL36G (56300)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474741
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56300 | IL36G | interleukin 36 gamma | NM_019618.4 | 100% | 100% | |
| 2 | human | 56300 | IL36G | interleukin 36 gamma | NM_001278568.2 | 79.2% | 78.6% | 55_56ins105 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 576
- ORF length:
- 507
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagaggcact cCAGGAGACG CTGATGGTGG AGGAAGGGCC GTCTATCAAT 121 CAATGTGTAA ACCTATTACT GGGACTATTA ATGATTTGAA TCAGCAAGTG TGGACCCTTC 181 AGGGTCAGAA CCTTGTGGCA GTTCCACGAA GTGACAGTGT GACCCCAGTC ACTGTTGCTG 241 TTATCACATG CAAGTATCCA GAGGCTCTTG AGCAAGGCAG AGGGGATCCC ATTTATTTGG 301 GAATCCAGAA TCCAGAAATG TGTTTGTATT GTGAGAAGGT TGGAGAACAG CCCACATTGC 361 AGCTAAAAGA GCAGAAGATC ATGGATCTGT ATGGCCAACC CGAGCCCGTG AAACCCTTCC 421 TTTTCTACCG TGCCAAGACT GGTAGGACCT CCACCCTTGA GTCTGTGGCC TTCCCGGACT 481 GGTTCATTGC CTCCTCCAAG AGAGACCAGC CCATCATTCT GACTTCAGAA CTTGGGAAGT 541 CATACAACAC TGCCTTTGAA TTAAATATAA ATGACTTGCC AACTTTCTTG TACAAAGTGG 601 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 661 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 721 ATACACGTCC ATCCGCTTCT ACACCACGCG TTAAGTCgac aatcaacctc tggattacaa 781 aatttgtgaa agatt