Construct: ORF TRCN0000474745
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008508.1_s317c1
- Derived from:
- ccsbBroadEn_03434
- DNA Barcode:
- CTGACGCGCGGGAAAGGTCGAAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TOMM7 (54543)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474745
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54543 | TOMM7 | translocase of outer mitoch... | NM_019059.4 | 100% | 100% | |
| 2 | mouse | 66169 | Tomm7 | translocase of outer mitoch... | NM_025394.3 | 90.3% | 94.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 314
- ORF end:
- 479
- ORF length:
- 165
- Sequence:
-
1 GTCGCTTCAT GTGACTCCAC GGAGTACCGG GCGCCGTCCA GGCACCTCGA TTAGTTCTCG 61 AGCTTTTGGA GTACGTCGTC TTTAGGTTGG GGGGAGGGGT TTTATGCGAT GGAGTTTCCC 121 CACACTGAGT GGGTGGAGAC TGAAGTTAGG CCAGCTTGGC ACTTGATGTA ATTCTCCTTG 181 GAATTTGCCC TTTTTGAGTT TGGATCTTGG TTCATTCTCA AGCCTCAGAC AGTGGTTCAA 241 AGTTTTTTTC TTCCATTTCA GGTGTCGTGA GGCTAGCATC GATTGATCAA CAAGTTTGTA 301 CAAAAAAGTT GGCATGGTGA AGCTGAGCAA AGAGGCCAAG CAGAGACTAC AGCAGCTCTT 361 CAAGGGGAGC CAGTTTGCCA TTCGCTGGGG CTTTATCCCT CTTGTGATTT ACCTGGGATT 421 TAAGAGGGGT GCAGATCCCG GAATGCCTGA ACCAACTGTT TTGAGCCTAC TTTGGGGATA 481 CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG 541 TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT 601 TTATATATCT TGTGGAAAGG ACGACTGACG CGCGGGAAAG GTCGAAACAC GCGTTAAGTC 661 gacaatcaac ctctggatta caaaatttgt gaaagatt