Construct: ORF TRCN0000474746
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014474.1_s317c1
- Derived from:
- ccsbBroadEn_00518
- DNA Barcode:
- GCTTGTAGTGTATATTCGTTTCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ETFA (2108)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474746
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2108 | ETFA | electron transfer flavoprot... | NM_000126.4 | 100% | 100% | |
| 2 | human | 2108 | ETFA | electron transfer flavoprot... | NM_001127716.1 | 85.2% | 85.2% | 38_39ins147 |
| 3 | human | 2108 | ETFA | electron transfer flavoprot... | XR_931766.3 | 63.7% | 1_81del;962_1115del;1235_1568del | |
| 4 | mouse | 110842 | Etfa | electron transferring flavo... | NM_145615.4 | 91.6% | 95.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1065
- ORF length:
- 999
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt ccgagcggcg gctccggggc agctccggcg ggcggcctca ttgctacgat 121 ttcagagtac cctggtaata gctgagcatg caaatgattc cctagcaccc attactttaa 181 ataccattac tgcagccaca cgccttggag gtgaagtgtc ctgcttagta gctggaacca 241 aatgtgacaa ggtggcacaa gatctctgta aagtagcagg catagcaaaa gttctggtgg 301 ctcagcatga tgtgtacaaa ggcctacttc cagaggaact gacaccattg attttggcaa 361 ctcagaagca gttcaattac acacacatct gtgctggagc atctgccttc ggaaagaacc 421 ttttgcccag agtagcagcc aaacttgagg ttgccccgat ttctgacatc attgcaatca 481 agtcacctga cacatttgtg agaactattt atgcaggaaa tgctctatgt acagtgaagt 541 gtgatgagaa agtgaaagtg ttttctgtcc gtggaacatc ctttgatgct gcagcaacaa 601 gtggcggtag tgccagttca gaaaaggCAT CAAGTACTTC ACCAGTGGAA ATATCAGAGT 661 GGCTTGACCA GAAATTAACA AAAAGTGATC GACCAGAGCT AACAGGTGCC AAAGTGGTGG 721 TATCTGGTGG TCGAGGCTTG AAGAGTGGAG AGAACTTTAA GTTGTTATAT GACTTGGCAG 781 ATCAACTACA TGCTGCAGTT GGTGCTTCCC GTGCTGCTGT TGATGCTGGC TTTGTTCCCA 841 ATGACATGCA AGTTGGACAG ACGGGAAAAA TAGTAGCACC AGAACTTTAT ATTGCTGTTG 901 GAATATCTGG AGCCATCCAA CATTTAGCTG GGATGAAAGA CAGCAAGACA ATTGTGGCAA 961 TTAATAAAGA CCCAGAAGCT CCAATTTTCC AAGTGGCAGA TTATGGAATA GTTGCAGATT 1021 TATTTAAGGT AGTTCCTGAA ATGACTGAGA TATTGAAGAA AAAATGCCCA ACTTTCTTGT 1081 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1141 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1201 GAAAGGACGA GCTTGTAGTG TATATTCGTT TCAAACGCGT TAAGTCgaca atcaacctct 1261 ggattacaaa atttgtgaaa gatt