Construct: ORF TRCN0000474746

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014474.1_s317c1
Derived from:
ccsbBroadEn_00518
DNA Barcode:
GCTTGTAGTGTATATTCGTTTCAA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ETFA (2108)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000474746
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2108 ETFA electron transfer flavoprot... NM_000126.4 100% 100%
2 human 2108 ETFA electron transfer flavoprot... NM_001127716.1 85.2% 85.2% 38_39ins147
3 human 2108 ETFA electron transfer flavoprot... XR_931766.3 63.7% 1_81del;962_1115del;1235_1568del
4 mouse 110842 Etfa electron transferring flavo... NM_145615.4 91.6% 95.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1065
ORF length:
999
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgtt ccgagcggcg gctccggggc agctccggcg ggcggcctca ttgctacgat
121ttcagagtac cctggtaata gctgagcatg caaatgattc cctagcaccc attactttaa
181ataccattac tgcagccaca cgccttggag gtgaagtgtc ctgcttagta gctggaacca
241aatgtgacaa ggtggcacaa gatctctgta aagtagcagg catagcaaaa gttctggtgg
301ctcagcatga tgtgtacaaa ggcctacttc cagaggaact gacaccattg attttggcaa
361ctcagaagca gttcaattac acacacatct gtgctggagc atctgccttc ggaaagaacc
421ttttgcccag agtagcagcc aaacttgagg ttgccccgat ttctgacatc attgcaatca
481agtcacctga cacatttgtg agaactattt atgcaggaaa tgctctatgt acagtgaagt
541gtgatgagaa agtgaaagtg ttttctgtcc gtggaacatc ctttgatgct gcagcaacaa
601gtggcggtag tgccagttca gaaaaggCAT CAAGTACTTC ACCAGTGGAA ATATCAGAGT
661GGCTTGACCA GAAATTAACA AAAAGTGATC GACCAGAGCT AACAGGTGCC AAAGTGGTGG
721TATCTGGTGG TCGAGGCTTG AAGAGTGGAG AGAACTTTAA GTTGTTATAT GACTTGGCAG
781ATCAACTACA TGCTGCAGTT GGTGCTTCCC GTGCTGCTGT TGATGCTGGC TTTGTTCCCA
841ATGACATGCA AGTTGGACAG ACGGGAAAAA TAGTAGCACC AGAACTTTAT ATTGCTGTTG
901GAATATCTGG AGCCATCCAA CATTTAGCTG GGATGAAAGA CAGCAAGACA ATTGTGGCAA
961TTAATAAAGA CCCAGAAGCT CCAATTTTCC AAGTGGCAGA TTATGGAATA GTTGCAGATT
1021TATTTAAGGT AGTTCCTGAA ATGACTGAGA TATTGAAGAA AAAATGCCCA ACTTTCTTGT
1081ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT
1141AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG
1201GAAAGGACGA GCTTGTAGTG TATATTCGTT TCAAACGCGT TAAGTCgaca atcaacctct
1261ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)