Construct: ORF TRCN0000474748
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010964.1_s317c1
- Derived from:
- ccsbBroadEn_02646
- DNA Barcode:
- ACCCATTTAAACCTTCATGAATCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ANXA10 (11199)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474748
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11199 | ANXA10 | annexin A10 | NM_007193.5 | 100% | 100% | |
| 2 | human | 11199 | ANXA10 | annexin A10 | XM_011531571.2 | 94.1% | 94.1% | 196_255del |
| 3 | mouse | 26359 | Anxa10 | annexin A10 | NM_001136089.2 | 87.5% | 88.8% | (many diffs) |
| 4 | mouse | 26359 | Anxa10 | annexin A10 | NM_011922.3 | 81.5% | 79.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1038
- ORF length:
- 972
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt ttgtggagac tatgtgcaag gaaccatctt cccagctccc aatttcaatc 121 ccataatgga tgcccaaatg ctaggaggag cactccaagg atttgactgt gacaaagaca 181 tgctgatcaa cattctgact cagcgctgca atgcacaaag gatgatgatt gcagaggcat 241 accagagcat gtatggccgg gacctgattg gggatatgag ggagcagctt tcggatcact 301 tcaaagatgt gatggctggc ctcatgtacc caccaccact gtatgatgct catgagctct 361 ggcatgccat gaagggagta ggcactgatg agaattgcct cattgaaata ctagcttcaa 421 gaacaaatgg agaaattttc cagatgcgag aagcctactg cttgcaatac agcaataacc 481 tccaagagga catttattca gagacctcag gacacttcag agatactctc atgaacttgg 541 tccaggggac cagagaggaa ggatatacag accctgcgat ggctgctcag gatgcaatgg 601 tcctatgggA AGCCTGTCAG CAGAAGACGG GGGAGCACAA AACCATGCTG CAAATGATCC 661 TGTGCAACAA GAGCTACCAG CAGCTGCGGC TGGTTTTCCA GGAATTTCAA AATATTTCTG 721 GGCAAGATAT GGTAGATGCC ATTAATGAAT GTTATGATGG ATACTTTCAG GAGCTGCTGG 781 TTGCAATTGT TCTCTGTGTT CGAGACAAAC CAGCCTATTT TGCTTATAGA TTATATAGTG 841 CAATTCATGA CTTTGGTTTC CATAATAAAA CTGTAATCAG GATTCTCATT GCCAGAAGTG 901 AAATAGACCT GCTGACCATA AGGAAACGAT ACAAAGAGCG ATATGGAAAA TCCCTATTTC 961 ATGATATCAG AAATTTTGCT TCAGGGCATT ATAAGAAAGC ACTGCTTGCC ATCTGTGCTG 1021 GTGATGCTGA GGACTACTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1081 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1141 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAACCCATT TAAACCTTCA 1201 TGAATCAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt