Construct: ORF TRCN0000474769
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001999.1_s317c1
- Derived from:
- ccsbBroadEn_04648
- DNA Barcode:
- TCAGACAAGATAACATCGCTGGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TEX261 (113419)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474769
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 113419 | TEX261 | testis expressed 261 | NM_144582.3 | 100% | 100% | |
| 2 | mouse | 21766 | Tex261 | testis expressed gene 261 | NM_009357.2 | 91.4% | 99.4% | (many diffs) |
| 3 | mouse | 21766 | Tex261 | testis expressed gene 261 | XM_006505886.2 | 69.8% | 76% | (many diffs) |
| 4 | mouse | 21766 | Tex261 | testis expressed gene 261 | XM_006505888.3 | 65.1% | 64.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 657
- ORF length:
- 588
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtggttcatg tacctgctga gctggctgtc gctcttcatc caggtggcct 121 tcatcacgct ggctgtcgcg gctggactct attacctggc agaactgata gaagaataca 181 cagtggccac cagcaggatc ataaaataCA TGATCTGGTT CTCCACCGCT GTACTGATTG 241 GCCTCTACGT CTTTGAGCGC TTCCCCACCA GCATGATTGG AGTGGGCCTA TTCACCAACC 301 TCGTCTACTT TGGCCTCCTC CAGACCTTCC CCTTCATCAT GCTGACCTCG CCTAACTTCA 361 TCCTGTCGTG TGGACTAGTG GTGGTGAATC ATTACCTAGC ATTTCAGTTT TTTGCAGAAG 421 AATATTATCC CTTCTCAGAG GTCCTGGCCT ATTTCACTTT CTGCCTGTGG ATAATTCCGT 481 TTGCGTTTTT TGTGTCACTT TCGGCCGGGG AGAACGTCCT GCCCTCTACC ATGCAGCCAG 541 GAGATGATGT CGTCTCCAAT TATTTCACCA AAGGCAAGCG GGGCAAACGC TTAGGGATCC 601 TGGTTGTCTT CTCCTTCATC AAAGAGGCCA TTCTACCCAG TCGTCAGAAG ATATACTTGC 661 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 721 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 781 ATATATCTTG TGGAAAGGAC GATCAGACAA GATAACATCG CTGGACACGC GTTAAGTCga 841 caatcaacct ctggattaca aaatttgtga aagatt