Transcript: Mouse XM_006505886.2

PREDICTED: Mus musculus testis expressed gene 261 (Tex261), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Tex261 (21766)
Length:
2944
CDS:
188..637

Additional Resources:

NCBI RefSeq record:
XM_006505886.2
NBCI Gene record:
Tex261 (21766)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006505886.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000126276 CAGCCGAATCATCAAATACAT pLKO.1 169 5UTR 100% 5.625 7.875 N Tex261 n/a
2 TRCN0000126277 CCTCTACGTCTTTGAGCGTTT pLKO.1 220 CDS 100% 4.050 5.670 N Tex261 n/a
3 TRCN0000288436 CCTCTACGTCTTTGAGCGTTT pLKO_005 220 CDS 100% 4.050 5.670 N Tex261 n/a
4 TRCN0000126275 CCGAATCATCAAATACATGAT pLKO.1 172 5UTR 100% 0.495 0.693 N Tex261 n/a
5 TRCN0000288516 CCGAATCATCAAATACATGAT pLKO_005 172 5UTR 100% 0.495 0.693 N Tex261 n/a
6 TRCN0000295761 GCTGATTCCCTTTCGAATAAA pLKO_005 1027 3UTR 100% 15.000 10.500 N Tex261 n/a
7 TRCN0000295760 CCCAGTCGGCAGAAGATATAC pLKO_005 614 CDS 100% 13.200 9.240 N Tex261 n/a
8 TRCN0000126278 GTGAATCATTACCTGGCATTT pLKO.1 362 CDS 100% 10.800 7.560 N Tex261 n/a
9 TRCN0000306963 GTGAATCATTACCTGGCATTT pLKO_005 362 CDS 100% 10.800 7.560 N Tex261 n/a
10 TRCN0000126274 GCTAACCTTCTGCCTAGACTA pLKO.1 1105 3UTR 100% 4.950 3.465 N Tex261 n/a
11 TRCN0000138246 CTCCTTCATCAAAGAGGCCAT pLKO.1 589 CDS 100% 2.160 1.512 N TEX261 n/a
12 TRCN0000135432 GTGGTGGTGAATCATTACCTA pLKO.1 356 CDS 100% 3.000 4.200 N TEX261 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006505886.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14352 pDONR223 100% 91.7% 99.3% None (many diffs) n/a
2 ccsbBroad304_14352 pLX_304 0% 91.7% 99.3% V5 (many diffs) n/a
3 TRCN0000478474 AGCATCACGACGAATGGGCCACTC pLX_317 74.7% 91.7% 99.3% V5 (many diffs) n/a
4 ccsbBroadEn_04648 pDONR223 100% 69.8% 76% None (many diffs) n/a
5 ccsbBroad304_04648 pLX_304 0% 69.8% 76% V5 (many diffs) n/a
6 TRCN0000474769 TCAGACAAGATAACATCGCTGGAC pLX_317 75.1% 69.8% 76% V5 (many diffs) n/a
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