Construct: ORF TRCN0000474784
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014582.1_s317c1
- Derived from:
- ccsbBroadEn_15438
- DNA Barcode:
- ACGTATCGAGCTAAGCGACCTGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GTF2H2 (2966)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474784
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2966 | GTF2H2 | general transcription facto... | NM_001364568.1 | 45.6% | 44% | (many diffs) |
| 2 | human | 2966 | GTF2H2 | general transcription facto... | NM_001364569.1 | 45.6% | 44% | (many diffs) |
| 3 | human | 2966 | GTF2H2 | general transcription facto... | NM_001364570.1 | 45.6% | 44% | (many diffs) |
| 4 | human | 2966 | GTF2H2 | general transcription facto... | NM_001364571.1 | 45.6% | 44% | (many diffs) |
| 5 | human | 728340 | GTF2H2C | GTF2H2 family member C | XM_011543615.1 | 42.3% | 40.4% | (many diffs) |
| 6 | human | 2966 | GTF2H2 | general transcription facto... | NM_001364567.1 | 41.5% | 40% | (many diffs) |
| 7 | human | 2966 | GTF2H2 | general transcription facto... | NM_001515.3 | 41.5% | 40% | (many diffs) |
| 8 | human | 2966 | GTF2H2 | general transcription facto... | XM_017009403.2 | 41.5% | 40% | (many diffs) |
| 9 | human | 2966 | GTF2H2 | general transcription facto... | XM_017009404.1 | 41.5% | 40% | (many diffs) |
| 10 | human | 2966 | GTF2H2 | general transcription facto... | XM_017009406.2 | 41.5% | 40% | (many diffs) |
| 11 | human | 730394 | GTF2H2C_2 | GTF2H2 family member C, copy 2 | NM_001042490.4 | 41.3% | 39.4% | (many diffs) |
| 12 | human | 728340 | GTF2H2C | GTF2H2 family member C | NM_001098728.2 | 41.3% | 39.4% | (many diffs) |
| 13 | human | 730394 | GTF2H2C_2 | GTF2H2 family member C, copy 2 | NM_001354437.1 | 41.3% | 39.4% | (many diffs) |
| 14 | human | 730394 | GTF2H2C_2 | GTF2H2 family member C, copy 2 | NM_001354438.1 | 41.3% | 39.4% | (many diffs) |
| 15 | human | 728340 | GTF2H2C | GTF2H2 family member C | XM_005248588.4 | 41.3% | 39.4% | (many diffs) |
| 16 | human | 728340 | GTF2H2C | GTF2H2 family member C | XM_005248590.3 | 41.3% | 39.4% | (many diffs) |
| 17 | human | 728340 | GTF2H2C | GTF2H2 family member C | XM_006714688.4 | 41.3% | 39.4% | (many diffs) |
| 18 | human | 2966 | GTF2H2 | general transcription facto... | NM_001364573.1 | 30% | 28.4% | (many diffs) |
| 19 | human | 2966 | GTF2H2 | general transcription facto... | NM_001364572.1 | 27.7% | 26.1% | (many diffs) |
| 20 | human | 728340 | GTF2H2C | GTF2H2 family member C | XM_005248592.3 | 27.1% | 25.3% | (many diffs) |
| 21 | human | 2966 | GTF2H2 | general transcription facto... | XM_024446037.1 | 27% | 25.5% | (many diffs) |
| 22 | human | 730394 | GTF2H2C_2 | GTF2H2 family member C, copy 2 | NM_001354439.1 | 26.9% | 25% | (many diffs) |
| 23 | human | 728340 | GTF2H2C | GTF2H2 family member C | XM_017009804.1 | 26.9% | 25% | (many diffs) |
| 24 | human | 653238 | GTF2H2B | general transcription facto... | NR_033417.1 | 10.2% | (many diffs) | |
| 25 | mouse | 23894 | Gtf2h2 | general transcription facto... | NM_022011.4 | 37.2% | 39.3% | (many diffs) |
| 26 | mouse | 23894 | Gtf2h2 | general transcription facto... | NM_001360706.1 | 37% | 39.1% | (many diffs) |
| 27 | mouse | 23894 | Gtf2h2 | general transcription facto... | XM_006517662.3 | 37% | 39.1% | (many diffs) |
| 28 | mouse | 23894 | Gtf2h2 | general transcription facto... | XR_873759.3 | 27.5% | (many diffs) | |
| 29 | mouse | 23894 | Gtf2h2 | general transcription facto... | NR_153794.1 | 26.4% | (many diffs) | |
| 30 | mouse | 23894 | Gtf2h2 | general transcription facto... | XR_873756.3 | 26.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 81
- ORF end:
- 576
- ORF length:
- 495
- Sequence:
-
1 GGTTCAAAGT TTTTTTCTTC CATTTCAGGT GTCGTGAGGC TAGCATCGAT TGATCAACAA 61 GTTTGTACAA AAAAGTTGGC ATGGATGAAG AACCTGAAAG AACTAAGCGA TGGGAAGGAG 121 GCTATGAAAG AACATGGGAG ATTCTTAAAG AAGATGAATC TGGATCACTT AAAGCTACAA 181 TAGAAGACAT TCTATTCAAG GCAAAGAGAA AAAGAGTATT TGAGCACCAT GGACAAGTTC 241 GACTTGGAAT GATGCGCCAC CTTTATGTGG TAGTAGATGG ATCAAGAACA ATGGAAGACC 301 AAGATTTAAA GCCTAATAGA CTGACGTGTA CTTTAAAGTT GTTGGAATAC TTTGTAGAGG 361 AATATTTTGA TCAAAATCCT ATTAGTCAGA TTGGAATAAT TGTAACTAAG AGTAAAAGAG 421 CTGAAAAATT GACTGAACTT TCAGGAAACC CAAGAAAACA TATAACGTCT TTGAAGAAAG 481 CTGTGGATAT GACCTGCCAT GGAGAGCCAT CTCTTTATAA TTCCCTAAGC ATAGCTATGC 541 AGACTCTAAA GTTAGTATTA TACATTATGT ATAATTGCCC AACTTTCTTG TACAAAGTGG 601 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 661 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 721 AACGTATCGA GCTAAGCGAC CTGACACGCG TTAAGTCgac aatcaacctc tggattacaa 781 aatttgtgaa agatt