Construct: ORF TRCN0000474794
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006007.1_s317c1
- Derived from:
- ccsbBroadEn_07097
- DNA Barcode:
- TAGATGCTTGAATGCCCGGACTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TRAF1 (7185)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474794
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7185 | TRAF1 | TNF receptor associated fac... | NM_001190945.1 | 99.9% | 100% | 1020C>T |
| 2 | human | 7185 | TRAF1 | TNF receptor associated fac... | NM_005658.5 | 99.9% | 100% | 1020C>T |
| 3 | human | 7185 | TRAF1 | TNF receptor associated fac... | NM_001190947.1 | 70.5% | 70.6% | 0_1ins366;654C>T |
| 4 | mouse | 22029 | Traf1 | TNF receptor-associated fac... | NM_001326601.1 | 84.2% | 84.3% | (many diffs) |
| 5 | mouse | 22029 | Traf1 | TNF receptor-associated fac... | NM_009421.4 | 84.2% | 84.3% | (many diffs) |
| 6 | mouse | 22029 | Traf1 | TNF receptor-associated fac... | XM_011239050.2 | 84.2% | 84.3% | (many diffs) |
| 7 | mouse | 22029 | Traf1 | TNF receptor-associated fac... | XM_011239051.2 | 84.2% | 84.3% | (many diffs) |
| 8 | mouse | 22029 | Traf1 | TNF receptor-associated fac... | XM_011239052.2 | 84.2% | 84.3% | (many diffs) |
| 9 | mouse | 22029 | Traf1 | TNF receptor-associated fac... | XM_011239053.2 | 84.2% | 84.3% | (many diffs) |
| 10 | mouse | 22029 | Traf1 | TNF receptor-associated fac... | XM_011239054.2 | 84.2% | 84.3% | (many diffs) |
| 11 | mouse | 22029 | Traf1 | TNF receptor-associated fac... | XM_017317129.1 | 84.2% | 84.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1314
- ORF length:
- 1248
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ctccagctca ggcagcagtc ctcgcccggc ccctgatgag aatgagtttc 121 cctttgggtg ccctcccacc gtctgccagg acccaaagga gcccagggct ctctgctgtg 181 caggctgtct ctctgagaac ccgaggaatg gcgaggatca gatctgcccc aaatgcagag 241 gggaagacct ccagtctata agcccaggaa gccgtcttcg aactcaggag aaggctcacc 301 ccgaggtggc tgaggctgga attgggtgcc cctttgcagg tgtcggctgc tccttcaagg 361 gaagcccaca gtctgtgcaa gagcatgagg tcacctccca gacctcccac ctaaacctgc 421 tgttggggtt catgaaacag tggaaggccc ggctgggctg tggcctggag tctgggccca 481 tggccctgga gcagaacctg tcagacctgc agctgcaggc agccgtggaa gtggcggggg 541 acctggaggt cgattgctac cgggcaccct gctccgagag ccaggaggag ctggccctgc 601 agcacttcat gaaggagaag cttctggctg agctggaggg gaagctgcgt gtgtttgaga 661 acattgttgc tgtcctcaac aaggaggtgg aggcctccca cctggccctg gccacctcta 721 tccaccagag ccagctggac cgtgagcgca tcctgagctt ggagcagagg gtggtggagc 781 ttcagcagac cctggcccag aaagaccagg ccctgggcaa gctggagcag agcttgcgcc 841 tcatggagga ggcctccttc gatggcactt tcctgtggaa gatcaccaat gtcaccaggc 901 ggtgccatga gtcggcctgt ggcaggaccg tcagcctctt ctccccagcc ttctacactg 961 ccaagtatgg ctacaagttg tgcctgcggc tgtaccTGAA TGGAGATGGC ACTGGAAAGA 1021 GAACCCATCT GTCGCTCTTC ATCGTGATCA TGAGAGGGGA GTATGATGCG CTGCTGCCGT 1081 GGCCTTTCCG GAACAAGGTC ACCTTCATGC TGCTGGACCA GAACAACCGT GAGCACGCCA 1141 TTGACGCCTT CCGGCCTGAC CTAAGCTCAG CGTCCTTCCA GAGGCCCCAG AGTGAAACCA 1201 ACGTGGCCAG TGGATGCCCA CTCTTCTTCC CCCTCAGCAA ACTGCAGTCA CCCAAGCACG 1261 CCTACGTGAA GGACGACACA ATGTTCCTCA AGTGCATTGT GGAGACCAGC ACTTACCCAA 1321 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1381 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1441 TATCTTGTGG AAAGGACGAT AGATGCTTGA ATGCCCGGAC TTCACGCGTT AAGTCgacaa 1501 tcaacctctg gattacaaaa tttgtgaaag att