Construct: ORF TRCN0000474828
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009174.1_s317c1
- Derived from:
- ccsbBroadEn_03683
- DNA Barcode:
- TCTCCCCATCTAGGCCCAATCAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- APOM (55937)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474828
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55937 | APOM | apolipoprotein M | NM_019101.3 | 100% | 100% | |
| 2 | human | 55937 | APOM | apolipoprotein M | XM_006715150.3 | 80.7% | 53.2% | 0_1ins103;167_173delGTGAGTG |
| 3 | human | 55937 | APOM | apolipoprotein M | NR_045828.2 | 67.5% | (many diffs) | |
| 4 | human | 55937 | APOM | apolipoprotein M | NM_001256169.2 | 61.7% | 61.7% | 0_1ins216 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 630
- ORF length:
- 564
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt ccaccaaatt tgggcagctc tgctctactt ctatggtatt atccttaact 121 ccatctacca gtgccctgag cacagtcaac tgacaactct gggcgtggat gggaaggagt 181 tcccagaggt ccacttgggc cagtggtact ttatcgcagg ggcagctccc accaaggagg 241 agttggcaac ttttgaccct gtggacaaca ttgtcttcaa tatggctgct ggctctgccc 301 cgatgcagct ccaccTTCGT GCTACCATCC GCATGAAAGA TGGGCTCTGT GTGCCCCGGA 361 AATGGATCTA CCACCTGACT GAAGGGAGCA CAGATCTCAG AACTGAAGGC CGCCCTGACA 421 TGAAGACTGA GCTCTTTTCC AGCTCATGCC CAGGTGGAAT CATGCTGAAT GAGACAGGCC 481 AGGGTTACCA GCGCTTTCTC CTCTACAATC GCTCACCACA TCCTCCCGAA AAGTGTGTGG 541 AGGAATTCAA GTCCCTGACT TCCTGCCTGG ACTCCAAAGC CTTCTTATTG ACTCCTAGGA 601 ATCAAGAGGC CTGTGAGCTG TCCAATAACT GCCCAACTTT CTTGTACAAA GTGGTTGATA 661 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 721 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATCTCC 781 CCATCTAGGC CCAATCAACA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 841 tgaaagatt