Construct: ORF TRCN0000474829
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013347.1_s317c1
- Derived from:
- ccsbBroadEn_12203
- DNA Barcode:
- ACTAAAGTGCGAAGCAAGCCTCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SPTLC3 (55304)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474829
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55304 | SPTLC3 | serine palmitoyltransferase... | XM_017027922.1 | 50% | 38.8% | (many diffs) |
| 2 | human | 55304 | SPTLC3 | serine palmitoyltransferase... | XR_001754317.1 | 30.8% | (many diffs) | |
| 3 | human | 55304 | SPTLC3 | serine palmitoyltransferase... | XM_011529279.1 | 29.4% | 19.4% | (many diffs) |
| 4 | human | 55304 | SPTLC3 | serine palmitoyltransferase... | NM_001349945.2 | 27.8% | 18.5% | (many diffs) |
| 5 | human | 55304 | SPTLC3 | serine palmitoyltransferase... | NM_018327.4 | 27.8% | 18.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 591
- ORF length:
- 525
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc taaccctgga ggtggtgctg tttgcaacgg gaaacttcac aatcacaaga 121 aacagagcaa tggctcacaa agcagaaact gcacaaagaa tggaatagtg aaggaagccc 181 agcaaaatgg gaagCCACAT TTTTATGATA AGCTCATTGT TGAATCGTTT GAGGAAGCAC 241 CCCTTCATGT TATGGTTTTC ACTTACATGG GATATGGAAT TGGAACCCTG TTTGGCTATC 301 TCAGAGACTT TTTAAGAAAC TGGGGAATAG AAAAATGCAA CGCAGCTGTG GAACGAAAAG 361 AACAAAAAGT ACGTATGCGC ACCTCCCTGG ATCTTTGTCA ATGCCTACTC CTCTCTAAAG 421 TGTTCTCAGA AGTGGTGATG CAGGTGCAGA TTCTAGAAAG CATGAGGTGC TCAGGAACTA 481 TTCAGGGCAA ATTTCATTCA TCTCCACCTG CTAAACCCCA TTACCCATGG GCTTATGGAC 541 CTGTTTTTAC AAACATCTCA TGGGCAACTA CTATTTGCCA CATACCAAAC TACTCAACTT 601 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 661 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 721 CTTGTGGAAA GGACGAACTA AAGTGCGAAG CAAGCCTCCT ACGCGTTAAG TCgacaatca 781 acctctggat tacaaaattt gtgaaagatt