Construct: ORF TRCN0000474834
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016431.1_s317c1
- Derived from:
- ccsbBroadEn_01514
- DNA Barcode:
- CCGAGTCATCCTTGGGCCGCATGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SDHC (6391)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474834
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6391 | SDHC | succinate dehydrogenase com... | NM_003001.3 | 100% | 100% | |
| 2 | human | 6391 | SDHC | succinate dehydrogenase com... | NM_001035512.2 | 79.8% | 79.8% | 77_78ins102 |
| 3 | human | 6391 | SDHC | succinate dehydrogenase com... | NM_001035513.2 | 68% | 65% | (many diffs) |
| 4 | human | 6391 | SDHC | succinate dehydrogenase com... | NM_001035511.2 | 55.8% | 46.5% | 240_241ins164;344_450del |
| 5 | human | 6391 | SDHC | succinate dehydrogenase com... | NM_001278172.2 | 39.2% | 29.5% | 77_78ins102;138_139ins164;242_348del |
| 6 | human | 6391 | SDHC | succinate dehydrogenase com... | NR_103459.2 | 3.7% | 1_25del;203_229del;560_13469del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 573
- ORF length:
- 507
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgcgctgttg ctgagacacg ttggtcgtca ttgcctccga gcccacttta 121 gccctcagct ctgtatcaga aatgctgttc ctttgggaac cacggccaaa gaagagatgg 181 agcggttctg gaataagaat ataggttcaa accgtccTCT GTCTCCCCAC ATTACTATCT 241 ACAGTTGGTC TCTTCCCATG GCGATGTCCA TCTGCCACCG TGGCACTGGT ATTGCTTTGA 301 GTGCAGGGGT CTCTCTTTTT GGCATGTCGG CCCTGTTACT CCCTGGGAAC TTTGAGTCTT 361 ATTTGGAACT TGTGAAGTCC CTGTGTCTGG GGCCAGCACT GATCCACACA GCTAAGTTTG 421 CACTTGTCTT CCCTCTCATG TATCATACCT GGAATGGGAT CCGACACTTG ATGTGGGACC 481 TAGGAAAAGG CCTGAAGATT CCCCAGCTAT ACCAGTCTGG AGTGGTTGTC CTGGTTCTTA 541 CTGTGTTGTC CTCTATGGGG CTGGCAGCCA TGTGCCCAAC TTTCTTGTAC AAAGTGGTTG 601 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 661 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACC 721 GAGTCATCCT TGGGCCGCAT GTACGCGTTA AGTCgacaat caacctctgg attacaaaat 781 ttgtgaaaga tt