Construct: ORF TRCN0000474854

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014200.1_s317c1
Derived from:
ccsbBroadEn_13992
DNA Barcode:
TCCGGCCCACTCTACGTTTGCATT
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
H3C4 (8351)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000474854
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 8351 H3C4 H3 clustered histone 4 NM_003530.4 99.7% 7.5% 11delC
2 human 8354 H3C11 H3 clustered histone 11 NM_003533.2 87.9% 7.5% (many diffs)
3 human 8355 H3C8 H3 clustered histone 8 NM_003534.2 87.9% 7.5% (many diffs)
4 human 8357 H3C10 H3 clustered histone 10 NM_003536.2 87.7% 7.5% (many diffs)
5 human 8968 H3C7 H3 clustered histone 7 NM_021018.2 87.7% 7.5% (many diffs)
6 human 8358 H3C2 H3 clustered histone 2 NM_003537.3 87.2% 7.5% (many diffs)
7 human 333932 H3C15 H3 clustered histone 15 NM_001005464.2 86.5% 7.5% (many diffs)
8 human 8353 H3C6 H3 clustered histone 6 NM_003532.2 86.5% 7.5% (many diffs)
9 human 126961 H3C14 H3 clustered histone 14 NM_021059.2 86.5% 7.5% (many diffs)
10 human 8350 H3C1 H3 clustered histone 1 NM_003529.2 86.2% 7.5% (many diffs)
11 human 653604 H3C13 H3 clustered histone 13 NM_001123375.2 86% 7.5% (many diffs)
12 human 8290 H3-4 H3.4 histone NM_003493.2 86% 5.7% (many diffs)
13 human 440686 H3-2 H3.2 histone (putative) NM_001355409.3 85.3% 7.5% (many diffs)
14 human 440686 H3-2 H3.2 histone (putative) NM_001372105.1 85.3% 7.5% (many diffs)
15 human 106479023 H3P4 H3 histone pseudogene 4 NR_160943.1 32.6% (many diffs)
16 human 106479023 H3P4 H3 histone pseudogene 4 NR_160941.1 17.8% (many diffs)
17 human 106479023 H3P4 H3 histone pseudogene 4 NR_160940.1 15.9% (many diffs)
18 mouse 319150 Hist1h3b histone cluster 1, H3b NM_178203.2 88.9% 7.5% (many diffs)
19 mouse 319153 Hist1h3i histone cluster 1, H3i NM_178207.2 88.9% 7.5% (many diffs)
20 mouse 319149 Hist1h3d histone cluster 1, H3d NM_178204.2 88.7% 7.5% (many diffs)
21 mouse 319152 Hist1h3h histone cluster 1, H3h NM_178206.2 88.7% 7.5% (many diffs)
22 mouse 360198 Hist1h3a histone cluster 1, H3a NM_013550.5 88.2% 7.5% (many diffs)
23 mouse 97908 Hist1h3g histone cluster 1, H3g NM_145073.2 88.2% 7.5% (many diffs)
24 mouse 319148 Hist1h3c histone cluster 1, H3c NM_175653.2 87.9% 7.5% (many diffs)
25 mouse 97114 Hist2h3c2 histone cluster 2, H3c2 NM_054045.4 87.7% 7.5% (many diffs)
26 mouse 15077 Hist2h3c1 histone cluster 2, H3c1 NM_178216.3 87.7% 7.5% (many diffs)
27 mouse 260423 Hist1h3f histone cluster 1, H3f NM_013548.4 87.5% 7.5% (many diffs)
28 mouse 319151 Hist1h3e histone cluster 1, H3e NM_178205.2 87.5% 7.5% (many diffs)
29 mouse 319154 Hist2h3b histone cluster 2, H3b NM_178215.2 87.5% 7.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
81
ORF end:
186
ORF length:
105
Sequence:
1GGTTCAAAGT TTTTTTCTTC CATTTCAGGT GTCGTGAGGC TAGCATCGAT TGATCAACAA
61GTTTGTACAA AAAAGTTGGC ATGGCTCGTA CAAGCAGACT GCTCGCAAGT CCACGGGTGG
121GAAAGCGCCA CGCAAGCAGC TGGCCACCAA GGCTGCTCGA AAGAGCGCTC CAGCCACCGG
181CGGCGTGAAG AAGCCCCACC GTTACCGGCC CGGCACGGTG GCTCTGCGCG AGATCCGCCG
241CTACCAGAAG TCGACCGAGC TGCTGATTCG CAAACTGCCA TTCCAGCGTC TAGTCCGTGA
301GATCGCGCAG GACTTCAAGA CTGATCTGCG TTTTCAGAGC TCGGCGGTGA TGGCGCTGCA
361GGAGGCCTGC GAGGCCTACC TGGTGGGGCT GTTTGAGGAC ACCAACCTAT GCGCCATTCA
421CGCCAAGCGA GTGACTATCA TGCCCAAGGA CATCCAGCTT GCTCGCCGCA TTCGTGGGGA
481GAGGGCGTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC
541TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT
601TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATCCGGCC CACTCTACGT TTGCATTACG
661CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)