Transcript: Human NM_001372105.1

Homo sapiens H3.2 histone (putative) (H3-2), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-09-17
Taxon:
Homo sapiens (human)
Gene:
H3-2 (440686)
Length:
1062
CDS:
12..422

Additional Resources:

NCBI RefSeq record:
NM_001372105.1
NBCI Gene record:
H3-2 (440686)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001372105.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000243829 AGGAAGCAGCTGGCTACCAAA pLKO_005 63 CDS 100% 4.950 2.475 Y H3C13 n/a
2 TRCN0000238060 ACCATCATGCCCAAGGACATC pLKO_005 366 CDS 100% 4.050 2.025 Y Hist1h3f n/a
3 TRCN0000092885 CATGCCCAAGGACATCCAGTT pLKO.1 371 CDS 100% 4.050 2.025 Y Hist2h3c2 n/a
4 TRCN0000243830 CATGCCCAAGGACATCCAGTT pLKO_005 371 CDS 100% 4.050 2.025 Y H3C13 n/a
5 TRCN0000265659 TTTAAGACGGACCTGCGCTTC pLKO_005 246 CDS 100% 2.250 1.125 Y H3C15 n/a
6 TRCN0000106758 AGACACGAACCTGTGCGCCAT pLKO.1 329 CDS 100% 0.720 0.360 Y H3C14 n/a
7 TRCN0000255652 CCAGCGGCTGGTACGCGAGAT pLKO_005 215 CDS 100% 0.000 0.000 Y H3C15 n/a
8 TRCN0000106756 CTGCCCTTCCAGCGGCTGGTA pLKO.1 207 CDS 100% 0.000 0.000 Y H3C14 n/a
9 TRCN0000282161 GTGAAGAAGCCGCACCGCTAC pLKO_005 117 CDS 100% 0.000 0.000 Y Hist2h3b n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001372105.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04820 pDONR223 100% 95.8% 97% None (many diffs) n/a
2 ccsbBroad304_04820 pLX_304 0% 95.8% 97% V5 (many diffs) n/a
3 TRCN0000470519 GCCGATTGAGCTCTTAAAAGGAAC pLX_317 100% 95.8% 97% V5 (many diffs) n/a
4 ccsbBroadEn_07214 pDONR223 100% 87.5% 94.1% None (many diffs) n/a
5 ccsbBroad304_07214 pLX_304 0% 87.5% 94.1% V5 (many diffs) n/a
6 TRCN0000477997 GCACAAGGTCGTGAATTCAGTTAA pLX_317 34.1% 87.5% 94.1% V5 (many diffs) n/a
7 ccsbBroadEn_13992 pDONR223 100% 85.3% 7.5% None (many diffs) n/a
8 ccsbBroad304_13992 pLX_304 0% 85.3% 7.5% V5 (not translated due to prior stop codon) (many diffs) n/a
9 TRCN0000474854 TCCGGCCCACTCTACGTTTGCATT pLX_317 100% 85.3% 7.5% V5 (not translated due to prior stop codon) (many diffs) n/a
10 ccsbBroadEn_01908 pDONR223 100% 85% 97% None (many diffs) n/a
11 ccsbBroad304_01908 pLX_304 0% 85% 97% V5 (many diffs) n/a
12 TRCN0000467485 CTCAATTAACCTTCGGAGAAGTTC pLX_317 89.4% 85% 97% V5 (many diffs) n/a
13 ccsbBroadEn_07225 pDONR223 100% 84.5% 96.3% None (many diffs) n/a
14 ccsbBroad304_07225 pLX_304 0% 84.5% 96.3% V5 (many diffs) n/a
15 ccsbBroadEn_07226 pDONR223 100% 84.5% 97% None (many diffs) n/a
16 ccsbBroad304_07226 pLX_304 0% 84.5% 97% V5 (many diffs) n/a
17 TRCN0000465999 CATGGCGATGGACTCTTGTAAGTC pLX_317 82.1% 84.5% 97% V5 (many diffs) n/a
18 ccsbBroadEn_07332 pDONR223 100% 84.5% 97% None (many diffs) n/a
19 ccsbBroad304_07332 pLX_304 0% 84.5% 97% V5 (many diffs) n/a
20 TRCN0000475169 CTGATGTGCTCCCTCCTTGCCCTC pLX_317 57.4% 84.5% 97% V5 (many diffs) n/a
21 ccsbBroadEn_07333 pDONR223 100% 84.3% 97% None (many diffs) n/a
22 ccsbBroad304_07333 pLX_304 0% 84.3% 97% V5 (many diffs) n/a
23 TRCN0000468120 GCAGCACCCCACCATAATCTTTGA pLX_317 96% 84.3% 97% V5 (many diffs) n/a
24 ccsbBroadEn_01906 pDONR223 100% 84.3% 97% None (many diffs) n/a
25 ccsbBroad304_01906 pLX_304 0% 84.3% 97% V5 (many diffs) n/a
26 TRCN0000469791 ACACCATCTCGTTTATTTCAGCTA pLX_317 77.1% 84.3% 97% V5 (many diffs) n/a
27 ccsbBroadEn_02054 pDONR223 100% 84.1% 97% None (many diffs) n/a
28 ccsbBroad304_02054 pLX_304 0% 84.1% 97% V5 (many diffs) n/a
29 TRCN0000474049 ACGAGTAGAGTAGCATGTAAGCCT pLX_317 92% 84.1% 97% V5 (many diffs) n/a
30 ccsbBroadEn_01907 pDONR223 100% 84.1% 97% None (many diffs) n/a
31 ccsbBroad304_01907 pLX_304 0% 84.1% 97% V5 (many diffs) n/a
32 TRCN0000469614 CTGCCGATCGGACTGCTGGCTACG pLX_317 100% 84.1% 97% V5 (many diffs) n/a
33 ccsbBroadEn_01904 pDONR223 100% 83.8% 97% None (many diffs) n/a
34 ccsbBroad304_01904 pLX_304 97.8% 83.8% 97% V5 (many diffs) n/a
35 TRCN0000465966 TGCACCAAATCGTATAGACTTAAT pLX_317 74.7% 83.8% 97% V5 (many diffs) n/a
36 ccsbBroadEn_01905 pDONR223 100% 83.3% 97% None (many diffs) n/a
37 ccsbBroad304_01905 pLX_304 0% 83.3% 97% V5 (many diffs) n/a
38 TRCN0000469866 TCGCGAGAACCCACGTCATTTGGC pLX_317 98.6% 83.3% 97% V5 (many diffs) n/a
39 ccsbBroadEn_01909 pDONR223 100% 81.3% 97% None (many diffs) n/a
40 ccsbBroad304_01909 pLX_304 97.2% 81.3% 97% V5 (many diffs) n/a
41 TRCN0000479054 GCGCGGCCAACGTGTTATAATTCG pLX_317 93.7% 81.3% 97% V5 (many diffs) n/a
42 ccsbBroadEn_00719 pDONR223 100% 80.1% 94.1% None (many diffs) n/a
43 ccsbBroad304_00719 pLX_304 0% 80.1% 94.1% V5 (many diffs) n/a
44 TRCN0000473475 ACAACCTAGAGAAGGGCCGCGTGA pLX_317 92% 80.1% 94.1% V5 (many diffs) n/a
45 ccsbBroadEn_10159 pDONR223 100% 78.2% 90.4% None (many diffs) n/a
46 ccsbBroad304_10159 pLX_304 0% 78.2% 90.4% V5 (many diffs) n/a
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