Construct: ORF TRCN0000474860
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006901.1_s317c1
- Derived from:
- ccsbBroadEn_14179
- DNA Barcode:
- ACACTCGTGGTCCCACACTGTTTC
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM120C (54954)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474860
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54954 | FAM120C | family with sequence simila... | NM_198456.3 | 35.2% | 12% | 1_425del;714_715ins107 |
| 2 | human | 54954 | FAM120C | family with sequence simila... | NM_001300788.2 | 13.7% | 3.6% | (many diffs) |
| 3 | human | 54954 | FAM120C | family with sequence simila... | XM_006724589.4 | 12.8% | 3.3% | (many diffs) |
| 4 | human | 54954 | FAM120C | family with sequence simila... | NM_017848.6 | 11.2% | 2.4% | (many diffs) |
| 5 | mouse | 207375 | Fam120c | family with sequence simila... | XM_006528776.3 | 28.5% | 7.9% | (many diffs) |
| 6 | mouse | 207375 | Fam120c | family with sequence simila... | XM_011247802.2 | 14.6% | 4.5% | (many diffs) |
| 7 | mouse | 207375 | Fam120c | family with sequence simila... | NM_198105.2 | 10.7% | 3.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 462
- ORF length:
- 396
- Sequence:
-
1 tcttcCATTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCATGGA ACGCCATGCT GGGCTACTTG TCAGCGCTGT GCCAGGCTTG TGCCTATCCT 121 GGCGGCGACG GCCTGGAGCT CGTGGTCATG TTCCCGGGGG GCCTGGGCAA GGACCGGCTG 181 GCCGAGTGGG GCCGTCGGTG CCAGGCCGAG CGGCAGACAG CGCAACTGAT CGTGGGACAC 241 GTGGGCAACA AGGGCACCCC TCCACCGCGG GCCTGGTTCC TGCCACCGGC CTGCCTGAGC 301 CACTGCGTGA GGCTAGCACT CATCCGCTTC CGGGTCAAGC AAGCTGCCAT GTTGTGAGCT 361 GCCCTATGGA GAGGCCCACA GGAAAAGAAA CTGAGGGAAG CCTCCAACCA ACACTCAGTG 421 AGGAACTGAG GCCCTCAGTC CAACTACAGC CTGCAAGGAA CTGCCAACTT TCTTGTACAA 481 AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA 541 ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA 601 GGACGAACAC TCGTGGTCCC ACACTGTTTC ACGCGTTAAG TCgacaatca acctctggat 661 tacaaaattt gtgaaagatt