Construct: ORF TRCN0000474868
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011577.1_s317c1
- Derived from:
- ccsbBroadEn_15742
- DNA Barcode:
- TGCGTGAGGTTCCTACTACGACGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GABARAPL2 (11345)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474868
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11345 | GABARAPL2 | GABA type A receptor associ... | NM_007285.7 | 99.7% | 100% | 153T>C |
| 2 | mouse | 93739 | Gabarapl2 | gamma-aminobutyric acid (GA... | NM_026693.5 | 93.1% | 100% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 104
- ORF end:
- 455
- ORF length:
- 351
- Sequence:
-
1 TTCATTCTCA AGCCTCAGAC AGTGGTTCAA AGTTTTTTTC TTCCATTTCA GGTGTCGTGA 61 GGCTAGCATC GATTGATCAA CAAGTTTGTA CAAAAAAGTT GGCATGAAGT GGATGTTCAA 121 GGAGGACCAC TCGCTGGAAC ACAGATGCGT GGAGTCCGCG AAGATTCGAG CGAAATATCC 181 CGACAGGGTT CCGGTGATTG TGGAAAAGGT CTCAGGCTCT CAGATTGTTG ACATTGACAA 241 ACGGAAGTAC TTGGTCCCAT CTGATATCAC TGTGGCTCAG TTCATGTGGA TCATCAGGAA 301 AAGGATCCAG CTTCCTTCTG AAAAGGCGAT CTTCCTGTTT GTGGATAAGA CAGTCCCACA 361 GTCCAGCCTA ACTATGGGAC AGCTTTACGA GAAGGAAAAA GATGAAGATG GATTCTTATA 421 TGTGGCCTAC AGCGGAGAGA ACACTTTTGG CTTCTGCCCA ACTTTCTTGT ACAAAGTGGT 481 TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC 541 TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA 601 TGCGTGAGGT TCCTACTACG ACGCACGCGT TAAGTCgaca atcaacctct ggattacaaa 661 atttgtgaaa gatt