Construct: ORF TRCN0000474876
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005840.1_s317c1
- Derived from:
- ccsbBroadEn_03497
- DNA Barcode:
- CCTTCCTAGACCTAGACTTGACTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TXNL4B (54957)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474876
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54957 | TXNL4B | thioredoxin like 4B | NM_001142317.1 | 100% | 100% | |
| 2 | human | 54957 | TXNL4B | thioredoxin like 4B | NM_001142318.2 | 100% | 100% | |
| 3 | human | 54957 | TXNL4B | thioredoxin like 4B | NM_017853.3 | 100% | 100% | |
| 4 | human | 54957 | TXNL4B | thioredoxin like 4B | NM_001324354.2 | 73.3% | 63.8% | (many diffs) |
| 5 | human | 54957 | TXNL4B | thioredoxin like 4B | XM_017023377.2 | 72.1% | 67.7% | (many diffs) |
| 6 | human | 54957 | TXNL4B | thioredoxin like 4B | NM_001324355.2 | 63.7% | 63.7% | 0_1ins162 |
| 7 | mouse | 234723 | Txnl4b | thioredoxin-like 4B | NM_175646.4 | 87.9% | 97.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 513
- ORF length:
- 447
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattGATC AACAAGTTTG TACAAAAAAG 61 TTGGCATGAG CTTCCTACTG CCCAAGCTGA CTAGCAAAAA GGAAGTAGAC CAGGCGATAA 121 AAAGTACTGC TGAGAAGGTG TTGGTTCTCA GGTTTGGGAG AGATGAAGAT CCTGTCTGTC 181 TGCAGCTAGA TGATATTCTT TCTAAGACCT CTTCTGACTT AAGTAAAATG GCTGCTATAT 241 ACCTGGTAGA TGTGGACCAA ACTGCAGTTT ATACACAGTA TTTTGACATC AGTTATATTC 301 CATCTACTGT CTTTTTCTTC AATGGGCAGC ATATGAAAGT GGATTATGGA TCTCCAGATC 361 ACACTAAGTT TGTGGGAAGC TTCAAAACCA AACAAGACTT CATAGATTTG ATTGAAGTAA 421 TCTATCGAGG AGCAATGAGG GGGAAGCTTA TTGTCCAAAG TCCTATTGAT CCCAAGAATA 481 TTCCCAAATA TGACCTTCTC TATCAAGACA TTTACCCAAC TTTCTTGTAC AAAGTGGTTG 541 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 601 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACC 661 TTCCTAGACC TAGACTTGAC TTACGCGTTA AGTCgacaat caacctctgg attacaaaat 721 ttgtgaaaga tt