Construct: ORF TRCN0000474881
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008607.1_s317c1
- Derived from:
- ccsbBroadEn_04924
- DNA Barcode:
- AGGTGCAAGCATGGCAACCTTTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAGEC3 (139081)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474881
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 139081 | MAGEC3 | MAGE family member C3 | NM_177456.2 | 100% | 100% | |
| 2 | human | 139081 | MAGEC3 | MAGE family member C3 | XM_011531267.3 | 100% | 100% | |
| 3 | human | 139081 | MAGEC3 | MAGE family member C3 | XM_017029265.2 | 100% | 100% | |
| 4 | human | 139081 | MAGEC3 | MAGE family member C3 | NM_138702.1 | 38.5% | 33.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1107
- ORF length:
- 1038
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcctctcttt ccaaaccttc cacgcctcag ctttgaggaa gacttccaga 121 acccgagtgt gacagaggac ttggtagatg cacaggattc catagatgag gaggaggagg 181 atgcctcctc cacttcctct tcctctttcc actttttatt cccctcctcc tcttccttgt 241 cctcatcctc acccttgtcc tcacccttac cctctactct cattctgggt gttccagaag 301 atgaggatat gcctgctgct gggatgccac ctcttcccca gagtcctcct gagattcctc 361 cccagggtcc tcccaagatc tctccccagg gtcctccgca gagtcctccc cagagtcctc 421 tagactcctg ctcatcccct cttttgtgga cccgattgga tgaggagtcc agcagtgaag 481 aggaggatac agctacttgg catgccttgc cagaaagtga atccttgccc aggtatgccc 541 tggatgaaaa ggtggctgag ttggtgcagt ttcttctcct caaatatcaa acaaaagagc 601 ctgtcacaaa ggcagagatg ctgacgactg tcatcaagaa gtataaggac tattttccca 661 tgatcttcgg gaaagcccat gagttcatag agctaatttt tggcattgcc ctgactgata 721 tggaccccga caaccactcc tatttctttg aagacacatt agacctcacc tatgagggaa 781 gcctgattga tgaccagggc atgcccaaga actgtctcct gattcTTATT CTCAGTATGA 841 TCTTCATAAA GGGCAGCTGT GTCCCCGAGG AGGTCATCTG GGAAGTGTTG AGTGCAATAG 901 GGGTGTGTGC TGGGAGGGAG CACTTTATAT ATGGGGATCC CAGAAAGCTG CTCACTATAC 961 ATTGGGTGCA GAGAAAGTAC CTGGAGTACC GGGAGGTGCC CAACAGTGCT CCTCCACGTT 1021 ATGAATTTTT GTGGGGTCCA AGAGCCCATT CAGAGGCCAG CAAGAGAAGT CTTAGAGTTT 1081 TTATCCAAGC TATCCAGTAT CATCCCTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG 1141 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1201 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAAGGTGCAA 1261 GCATGGCAAC CTTTTTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1321 aagatt