Construct: ORF TRCN0000474900

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016564.1_s317c1
Derived from:
ccsbBroadEn_08366
DNA Barcode:
CCGATCATTGATCTAACTATTGGG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GPR173 (54328)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000474900
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 54328 GPR173 G protein-coupled receptor 173 NM_018969.5 99.8% 100% 87G>A;1062C>T
2 human 54328 GPR173 G protein-coupled receptor 173 XM_011530798.2 99.8% 100% 87G>A;1062C>T
3 mouse 70771 Gpr173 G-protein coupled receptor 173 NM_001313748.1 94.6% 99.4% (many diffs)
4 mouse 70771 Gpr173 G-protein coupled receptor 173 NM_027543.4 94.6% 99.4% (many diffs)
5 mouse 70771 Gpr173 G-protein coupled receptor 173 XM_006528981.3 94.6% 99.4% (many diffs)
6 mouse 70771 Gpr173 G-protein coupled receptor 173 XM_006528983.3 94.6% 99.4% (many diffs)
7 mouse 70771 Gpr173 G-protein coupled receptor 173 XM_011247871.2 94.6% 99.4% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1185
ORF length:
1119
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc caacactacc ggagagcctg aggaggtgag cggcgctctg tccccaccgt
121ccgcatcagc ttatgtgaag ctggtactgc taggactgat tatgtgcgtg agcctggcgg
181gtaacgccat cttgtccctg ctggtgctca aggagcgtgc cctgcacaag gctccttact
241acttcctgct ggacctgtgc ctggccgatg gcatacgctc tgccgtctgc ttcccctttg
301tgctggcttc tgtgcgccac ggctcttcat ggaccttcag tgcactcagc tgcaagattg
361tggcctttat ggccgtgctc ttttgcttcc atgcggcctt catgctgttc tgcatcagcg
421tcacccgcta catggccatc gcccaccacc gcttctacgc caagcgcatg acactctgga
481catgcgcggc tgtcatctgc atggcctgga ccctgtctgt ggccatggcc ttcccacctg
541tctttgacgt gggcacctac aagtttattc gggaggagga ccagtgcatc tttgagcatc
601gctacttcaa ggccaatgac acgctgggct tcatgcttat gttggctgtg ctcatggcag
661ctacccatgc tgtctacggc aagctgctcc tcttcgagta tcgtcaccgc aagatgaagc
721cagtgcagat ggtgccagcc aTCAGCCAGA ACTGGACATT CCATGGTCCC GGGGCCACCG
781GCCAGGCTGC TGCCAACTGG ATCGCCGGCT TTGGCCGTGG GCCCATGCCA CCAACCCTGC
841TGGGTATCCG GCAGAATGGG CATGCAGCCA GCCGGCGGCT ACTGGGCATG GACGAGGTCA
901AGGGTGAAAA GCAGCTGGGC CGCATGTTCT ACGCGATCAC ACTGCTCTTT CTGCTCCTCT
961GGTCACCCTA CATCGTGGCC TGCTACTGGC GAGTGTTTGT GAAAGCCTGT GCTGTGCCCC
1021ACCGCTACCT GGCCACTGCT GTTTGGATGA GCTTCGCCCA GGCTGCCGTC AACCCAATTG
1081TCTGCTTCCT GCTCAACAAG GACCTCAAGA AGTGCCTGAG GACTCATGCC CCCTGCTGGG
1141GCACAGGAGG TGCCCCGGCT CCCAGAGAAC CCTACTGTGT CATGTGCCCA ACTTTCTTGT
1201ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT
1261AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG
1321GAAAGGACGA CCGATCATTG ATCTAACTAT TGGGACGCGT TAAGTCgaca atcaacctct
1381ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)