Construct: ORF TRCN0000474900
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016564.1_s317c1
- Derived from:
- ccsbBroadEn_08366
- DNA Barcode:
- CCGATCATTGATCTAACTATTGGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GPR173 (54328)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474900
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54328 | GPR173 | G protein-coupled receptor 173 | NM_018969.5 | 99.8% | 100% | 87G>A;1062C>T |
| 2 | human | 54328 | GPR173 | G protein-coupled receptor 173 | XM_011530798.2 | 99.8% | 100% | 87G>A;1062C>T |
| 3 | mouse | 70771 | Gpr173 | G-protein coupled receptor 173 | NM_001313748.1 | 94.6% | 99.4% | (many diffs) |
| 4 | mouse | 70771 | Gpr173 | G-protein coupled receptor 173 | NM_027543.4 | 94.6% | 99.4% | (many diffs) |
| 5 | mouse | 70771 | Gpr173 | G-protein coupled receptor 173 | XM_006528981.3 | 94.6% | 99.4% | (many diffs) |
| 6 | mouse | 70771 | Gpr173 | G-protein coupled receptor 173 | XM_006528983.3 | 94.6% | 99.4% | (many diffs) |
| 7 | mouse | 70771 | Gpr173 | G-protein coupled receptor 173 | XM_011247871.2 | 94.6% | 99.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1185
- ORF length:
- 1119
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc caacactacc ggagagcctg aggaggtgag cggcgctctg tccccaccgt 121 ccgcatcagc ttatgtgaag ctggtactgc taggactgat tatgtgcgtg agcctggcgg 181 gtaacgccat cttgtccctg ctggtgctca aggagcgtgc cctgcacaag gctccttact 241 acttcctgct ggacctgtgc ctggccgatg gcatacgctc tgccgtctgc ttcccctttg 301 tgctggcttc tgtgcgccac ggctcttcat ggaccttcag tgcactcagc tgcaagattg 361 tggcctttat ggccgtgctc ttttgcttcc atgcggcctt catgctgttc tgcatcagcg 421 tcacccgcta catggccatc gcccaccacc gcttctacgc caagcgcatg acactctgga 481 catgcgcggc tgtcatctgc atggcctgga ccctgtctgt ggccatggcc ttcccacctg 541 tctttgacgt gggcacctac aagtttattc gggaggagga ccagtgcatc tttgagcatc 601 gctacttcaa ggccaatgac acgctgggct tcatgcttat gttggctgtg ctcatggcag 661 ctacccatgc tgtctacggc aagctgctcc tcttcgagta tcgtcaccgc aagatgaagc 721 cagtgcagat ggtgccagcc aTCAGCCAGA ACTGGACATT CCATGGTCCC GGGGCCACCG 781 GCCAGGCTGC TGCCAACTGG ATCGCCGGCT TTGGCCGTGG GCCCATGCCA CCAACCCTGC 841 TGGGTATCCG GCAGAATGGG CATGCAGCCA GCCGGCGGCT ACTGGGCATG GACGAGGTCA 901 AGGGTGAAAA GCAGCTGGGC CGCATGTTCT ACGCGATCAC ACTGCTCTTT CTGCTCCTCT 961 GGTCACCCTA CATCGTGGCC TGCTACTGGC GAGTGTTTGT GAAAGCCTGT GCTGTGCCCC 1021 ACCGCTACCT GGCCACTGCT GTTTGGATGA GCTTCGCCCA GGCTGCCGTC AACCCAATTG 1081 TCTGCTTCCT GCTCAACAAG GACCTCAAGA AGTGCCTGAG GACTCATGCC CCCTGCTGGG 1141 GCACAGGAGG TGCCCCGGCT CCCAGAGAAC CCTACTGTGT CATGTGCCCA ACTTTCTTGT 1201 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1261 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1321 GAAAGGACGA CCGATCATTG ATCTAACTAT TGGGACGCGT TAAGTCgaca atcaacctct 1381 ggattacaaa atttgtgaaa gatt