Construct: ORF TRCN0000474904

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008701.2_s317c1
Derived from:
ccsbBroadEn_16002
DNA Barcode:
AGTGTCAATTGTCCCAGGATATAG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
HKDC1 (80201)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000474904
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 80201 HKDC1 hexokinase domain containing 1 NM_025130.4 46.4% 46.4% 1_1470del;1803C>T;2161T>C
2 human 80201 HKDC1 hexokinase domain containing 1 XR_001747209.1 33.5% (many diffs)
3 human 80201 HKDC1 hexokinase domain containing 1 XM_011540195.2 29.6% 27.6% (many diffs)
4 mouse 216019 Hkdc1 hexokinase domain containing 1 XM_011243398.2 54.6% 57.7% (many diffs)
5 mouse 216019 Hkdc1 hexokinase domain containing 1 NM_145419.1 40.3% 42.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1347
ORF length:
1281
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgcg ggctgagctg gagtatgggc tgaagaagaa gagccacggg ctggccacgg
121tcaggatgct gcccacctac gtctgcgggc tgccggacgg cacagagaaa ggaaagtttc
181tcgccctgga tcttggggga accaacttcc gggtcctcct ggtgaagatc agaagtggac
241ggaggtcagt gcgaatgtac aacaagatct tcgccatccc cctggagatc atgcagggca
301ctggtgagga gctctttgat cacattgtgc agtgcatcgc cgacttcctg gactacatgg
361gcctcaaggg agcctcccta cctttgggct tcacattttc atttccctgc aggcagatga
421gcattgacaa gggaacactc atagggtgga ccaaaggttt caaggccact gactgtgaag
481gggaggacgt ggtggacatg ctcagggaag ccatcaagag gagaaacgag tttgacctgg
541acattgttgc agtcgtgaat gatacagtgg ggaccatgat gacctgtggc tatgaagatc
601ctaattgtga gattggcctg attgcaggaa caggcagcaa catgtgctac atggaggaca
661tgaggaacat cgagatggtg gaggggggtg aagggaagat gtgcatcaat acagagtggg
721gaggatttgg agacaatggc tgcatagatg acatccggac ccgatacgac acggaggtgg
781atgaggggtc cttgaatcct ggcaagcaga gatacgagaa aatgaccagt gggatgtact
841tgggggagat tgtgcggcag atcctgatcg acctgaccaa gcagggtctc ctcttccgag
901ggcagatttc agagcgtctc cggaccaggg gcatcttcga aaccaagttc ctgTCCCAGA
961TCGAAAGCGA TCGGCTGGCC CTTCTCCAGG TCAGGAGGAT TCTGCAGCAG CTGGGCCTGG
1021ACAGCACGTG TGAGGACAGC ATCGTGGTGA AGGAGGTGTG CGGAGCCGTG TCCCGGCGGG
1081CGGCCCAGCT CTGCGGTGCT GGCCTGGCCG CTATAGTGGA AAAAAGGAGA GAAGACCAGG
1141GGCTAGAGCA CCTGAGGATC ACTGTGGGTG TGGACGGCAC CCTGTACAAG CTGCACCCTC
1201ACTTTTCTAG AATATTGCAG GAAACTGTGA AGGAACTAGC CCCTCGATGT GATGTGACAT
1261TCATGCTGTC AGAAGATGGC AGTGGAAAAG GGGCAGCACT GATCACTGCT GTGGCCAAGA
1321GGTTACAGCA GGCACAGAAG GAGAACTACC CAACTTTCTT GTACAAAGTG GTTGATATCG
1381GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT
1441AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAAGTGTCAA
1501TTGTCCCAGG ATATAGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga
1561aagatt

Download FASTA (ORF) (Full)