Construct: ORF TRCN0000474904
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008701.2_s317c1
- Derived from:
- ccsbBroadEn_16002
- DNA Barcode:
- AGTGTCAATTGTCCCAGGATATAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HKDC1 (80201)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474904
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80201 | HKDC1 | hexokinase domain containing 1 | NM_025130.4 | 46.4% | 46.4% | 1_1470del;1803C>T;2161T>C |
| 2 | human | 80201 | HKDC1 | hexokinase domain containing 1 | XR_001747209.1 | 33.5% | (many diffs) | |
| 3 | human | 80201 | HKDC1 | hexokinase domain containing 1 | XM_011540195.2 | 29.6% | 27.6% | (many diffs) |
| 4 | mouse | 216019 | Hkdc1 | hexokinase domain containing 1 | XM_011243398.2 | 54.6% | 57.7% | (many diffs) |
| 5 | mouse | 216019 | Hkdc1 | hexokinase domain containing 1 | NM_145419.1 | 40.3% | 42.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1347
- ORF length:
- 1281
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcg ggctgagctg gagtatgggc tgaagaagaa gagccacggg ctggccacgg 121 tcaggatgct gcccacctac gtctgcgggc tgccggacgg cacagagaaa ggaaagtttc 181 tcgccctgga tcttggggga accaacttcc gggtcctcct ggtgaagatc agaagtggac 241 ggaggtcagt gcgaatgtac aacaagatct tcgccatccc cctggagatc atgcagggca 301 ctggtgagga gctctttgat cacattgtgc agtgcatcgc cgacttcctg gactacatgg 361 gcctcaaggg agcctcccta cctttgggct tcacattttc atttccctgc aggcagatga 421 gcattgacaa gggaacactc atagggtgga ccaaaggttt caaggccact gactgtgaag 481 gggaggacgt ggtggacatg ctcagggaag ccatcaagag gagaaacgag tttgacctgg 541 acattgttgc agtcgtgaat gatacagtgg ggaccatgat gacctgtggc tatgaagatc 601 ctaattgtga gattggcctg attgcaggaa caggcagcaa catgtgctac atggaggaca 661 tgaggaacat cgagatggtg gaggggggtg aagggaagat gtgcatcaat acagagtggg 721 gaggatttgg agacaatggc tgcatagatg acatccggac ccgatacgac acggaggtgg 781 atgaggggtc cttgaatcct ggcaagcaga gatacgagaa aatgaccagt gggatgtact 841 tgggggagat tgtgcggcag atcctgatcg acctgaccaa gcagggtctc ctcttccgag 901 ggcagatttc agagcgtctc cggaccaggg gcatcttcga aaccaagttc ctgTCCCAGA 961 TCGAAAGCGA TCGGCTGGCC CTTCTCCAGG TCAGGAGGAT TCTGCAGCAG CTGGGCCTGG 1021 ACAGCACGTG TGAGGACAGC ATCGTGGTGA AGGAGGTGTG CGGAGCCGTG TCCCGGCGGG 1081 CGGCCCAGCT CTGCGGTGCT GGCCTGGCCG CTATAGTGGA AAAAAGGAGA GAAGACCAGG 1141 GGCTAGAGCA CCTGAGGATC ACTGTGGGTG TGGACGGCAC CCTGTACAAG CTGCACCCTC 1201 ACTTTTCTAG AATATTGCAG GAAACTGTGA AGGAACTAGC CCCTCGATGT GATGTGACAT 1261 TCATGCTGTC AGAAGATGGC AGTGGAAAAG GGGCAGCACT GATCACTGCT GTGGCCAAGA 1321 GGTTACAGCA GGCACAGAAG GAGAACTACC CAACTTTCTT GTACAAAGTG GTTGATATCG 1381 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1441 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAAGTGTCAA 1501 TTGTCCCAGG ATATAGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1561 aagatt