Construct: ORF TRCN0000474915
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001092.1_s317c1
- Derived from:
- ccsbBroadEn_01236
- DNA Barcode:
- GGCCCGATAGCTATAACCCTCTGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- POLE2 (5427)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474915
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5427 | POLE2 | DNA polymerase epsilon 2, a... | NM_002692.4 | 100% | 100% | |
| 2 | human | 5427 | POLE2 | DNA polymerase epsilon 2, a... | NM_001348384.1 | 99.8% | 99.8% | 1071_1072insAGT |
| 3 | human | 5427 | POLE2 | DNA polymerase epsilon 2, a... | XM_017021374.1 | 95.8% | 95.6% | (many diffs) |
| 4 | human | 5427 | POLE2 | DNA polymerase epsilon 2, a... | NM_001197331.2 | 95.1% | 94.6% | 1499_1499delTinsGCTCTTTTCC;1504_1505insG;1506_1507ins65 |
| 5 | human | 5427 | POLE2 | DNA polymerase epsilon 2, a... | NM_001197330.1 | 95% | 95% | 245_246ins78 |
| 6 | human | 5427 | POLE2 | DNA polymerase epsilon 2, a... | XM_011536842.2 | 91% | 90.8% | (many diffs) |
| 7 | human | 5427 | POLE2 | DNA polymerase epsilon 2, a... | XR_943479.1 | 90.1% | 1_11del;1025_1026ins55;1538_1637del | |
| 8 | human | 5427 | POLE2 | DNA polymerase epsilon 2, a... | NM_001348385.1 | 84.9% | 84.6% | 0_1ins235;8_11delTATG |
| 9 | human | 5427 | POLE2 | DNA polymerase epsilon 2, a... | XM_011536843.2 | 81.2% | 80.7% | (many diffs) |
| 10 | human | 5427 | POLE2 | DNA polymerase epsilon 2, a... | XR_943480.1 | 79.9% | (many diffs) | |
| 11 | human | 5427 | POLE2 | DNA polymerase epsilon 2, a... | XR_001750377.1 | 65.2% | (many diffs) | |
| 12 | human | 5427 | POLE2 | DNA polymerase epsilon 2, a... | XR_001750378.1 | 62.9% | (many diffs) | |
| 13 | human | 5427 | POLE2 | DNA polymerase epsilon 2, a... | XM_011536844.1 | 50.2% | 48.5% | 754_755ins173;795_796ins613 |
| 14 | mouse | 18974 | Pole2 | polymerase (DNA directed), ... | NM_011133.2 | 86.2% | 90.1% | (many diffs) |
| 15 | mouse | 18974 | Pole2 | polymerase (DNA directed), ... | XM_011244018.1 | 76.9% | 80.6% | (many diffs) |
| 16 | mouse | 18974 | Pole2 | polymerase (DNA directed), ... | XM_006515598.3 | 70% | 68.4% | (many diffs) |
| 17 | mouse | 18974 | Pole2 | polymerase (DNA directed), ... | XM_006515599.1 | 46.1% | 45.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1650
- ORF length:
- 1581
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcgccggag cggctgcgga gccgggcgct ctccgccttc aagttgcggg 121 gcttgctgct ccgtggtgaa gctattaagt acctcacaga agctcttcag tctatcagtg 181 aattagagct tgaagataaa ctggaaaaga taattaatgc agttgagaag caacccttgt 241 catcaaacat gattgaacga tctgtggtgg aagcagcagt ccaggaatgc agtcagtctg 301 ttgatgaaac tatagagcac gttttcaata tcataggagc atttgatatt ccacgctttg 361 tgtacaattc agaaagaaaa aaatttcttc ctctgttaat gaccaaccac cctgcaccaa 421 atttatttgg aacaccaaga gataaagcag agatgtttcg tgagcgatat accattttgc 481 accagaggac ccacaggcat gaattattta ctcctccggt gataggttct caccctgatg 541 aaagcggaag caaattccag cttaaaacaa tagaaacctt attgggtagt acaaccaaaa 601 tcggagatgc gattgttctt ggaatgataa cgcagttaaa agagggaaaa ttttttctgg 661 aagatcctac tggaacagtc caactagacc ttagtaaagc tcagttccat agtggtttat 721 acacagaggc atgctttgtc ttagcagaag gttggtttga agatcaagtg tttcatgtca 781 atgcctttgg atttccaccc actgagccct ctagtactac tagggcatac tatggaaata 841 ttaatttttt tggaggtcct tctaatacat ctgtgaagac ttctgcaaaa ctaaaacagc 901 tagaagagga gaataaagat gctatgtttg tgtttttatc tgatgtttgg ttggaccagg 961 tggaagtatt ggaaaaactt cgcataatgt ttgctggtta ttcaccagca cctccaacct 1021 gctttattct gtgtggtaat ttttcatctg caccatatgg AAAAAATCAA GTTCAAGCTT 1081 TGAAAGATTC CCTAAAAACT TTGGCAGATA TAATATGTGA ATACCCAGAT ATTCACCAAA 1141 GTAGTCGTTT TGTGTTTGTA CCTGGTCCAG AGGATCCTGG ATTTGGTTCC ATCTTACCAA 1201 GGCCACCACT TGCTGAAAGC ATCACTAATG AATTCAGACA AAGGGTACCA TTTTCAGTTT 1261 TTACTACTAA TCCTTGCAGA ATTCAGTACT GTACACAGGA AATTACTGTC TTCCGTGAAG 1321 ACTTAGTAAA TAAAATGTGC AGAAACTGCG TCCGTTTTCC TAGCAGCAAT TTGGCTATTC 1381 CTAATCACTT TGTAAAGACT ATCTTATCCC AAGGACATCT GACTCCCCTA CCTCTTTATG 1441 TCTGCCCAGT GTATTGGGCA TATGACTATG CTTTGAGAGT GTATCCTGTG CCCGATCTAC 1501 TTGTCATTGC AGACAAATAT GATCCTTTCA CTACGACAAA TACCGAATGC CTCTGCATAA 1561 ACCCTGGCTC TTTTCCAAGA AGTGGATTTT CATTCAAAGT TTTTTATCCT TCTAATAAGA 1621 CAGTAGAAGA TAGCAAACTT CAAGGCTTTT TGCCAACTTT CTTGTACAAA GTGGTTGATA 1681 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1741 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGGCCC 1801 GATAGCTATA ACCCTCTGAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1861 tgaaagatt