Construct: ORF TRCN0000474923
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010947.1_s317c1
- Derived from:
- ccsbBroadEn_08397
- DNA Barcode:
- TTTTATCTCCATCATCTCCCATAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ARL15 (54622)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474923
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54622 | ARL15 | ADP ribosylation factor lik... | NM_019087.3 | 99.8% | 100% | 15A>G |
| 2 | human | 54622 | ARL15 | ADP ribosylation factor lik... | XM_017009598.1 | 95.9% | 93.6% | (many diffs) |
| 3 | human | 54622 | ARL15 | ADP ribosylation factor lik... | XM_011543500.2 | 91.3% | 91.4% | 15A>G;48_104del |
| 4 | human | 54622 | ARL15 | ADP ribosylation factor lik... | XM_011543499.2 | 78.5% | 74.4% | (many diffs) |
| 5 | human | 54622 | ARL15 | ADP ribosylation factor lik... | XM_011543498.2 | 74.7% | 72.8% | (many diffs) |
| 6 | mouse | 218639 | Arl15 | ADP-ribosylation factor-lik... | NM_172595.4 | 90.5% | 97% | (many diffs) |
| 7 | mouse | 218639 | Arl15 | ADP-ribosylation factor-lik... | XM_006517628.3 | 85.8% | 85.9% | (many diffs) |
| 8 | mouse | 218639 | Arl15 | ADP-ribosylation factor-lik... | XM_011244655.2 | 84.3% | 88.8% | (many diffs) |
| 9 | mouse | 218639 | Arl15 | ADP-ribosylation factor-lik... | XM_006517630.3 | 81.3% | 87.7% | (many diffs) |
| 10 | mouse | 218639 | Arl15 | ADP-ribosylation factor-lik... | XM_006517629.3 | 78.1% | 71.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 678
- ORF length:
- 612
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc tgatctccgg ataactgagg cgtttctgta catggattat ctgtgtttta 121 gagcactttg ctgcaaggga ccaccacctg cacgaccaga atatgacctg gtttgcatag 181 gcctcacagg ttctggcaaa accagtctgt tgtccaaact ctgcagtgaa agccccgata 241 acgtcgtgtc gaccacaggt tttagtatta aagcagtgcc attccagaat gccatcttga 301 atgtaaaaga acttggaggg gctgataaca tccggaaata ctGGAGCCGC TACTACCAAG 361 GATCTCAAGG GGTAATATTT GTATTAGACA GTGCCTCTTC AGAGGATGAT TTAGAAGCTG 421 CTAGAAATGA GCTGCACTCA GCTCTTCAGC ATCCACAGTT ATGCACTTTA CCCTTTTTAA 481 TATTGGCCAA TCATCAAGAC AAGCCAGCAG CTCGCTCAGT ACAAGAGATC AAAAAATATT 541 TTGAACTTGA ACCACTTGCA CGTGGAAAAC GCTGGATTCT ACAGCCCTGC TCACTGGATG 601 ACATGGATGC ACTGAAAGAC AGCTTCTCTC AGCTGATTAA TTTGTTAGAA GAAAAAGACC 661 ATGAAGCTGT AAGAATGTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 721 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 781 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATTTTATC TCCATCATCT 841 CCCATATACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt