Construct: ORF TRCN0000474926
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017437.1_s317c1
- Derived from:
- ccsbBroadEn_03664
- DNA Barcode:
- GATTCCGCGCAGTGTCTGTAAGAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BRK1 (55845)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474926
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55845 | BRK1 | BRICK1 subunit of SCAR/WAVE... | NM_018462.5 | 100% | 100% | |
| 2 | mouse | 101314 | Brk1 | BRICK1, SCAR/WAVE actin-nuc... | NM_133937.1 | 94.6% | 98.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 291
- ORF length:
- 225
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttG TACAAAAAAG 61 TTGGCATGGC GGGACAGGAG GATCCGGTGC AGCGGGAGAT TCACCAGGAC TGGGCTAACC 121 GGGAGTACAT TGAGATAATC ACCAGCAGCA TCAAGAAAAT CGCAGACTTT CTCAACTCGT 181 TCGATATGTC TTGTCGTTCA AGACTTGCAA CACTAAACGA GAAATTGACA GCCCTTGAAC 241 GGAGAATAGA GTACATTGAA GCTCGGGTGA CAAAAGGTGA GACACTCACC TACCCAACTT 301 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 361 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 421 CTTGTGGAAA GGACGAGATT CCGCGCAGTG TCTGTAAGAA ACGCGTTAAG TCgacaatca 481 acctctggat tacaaaattt gtgaaagatt