Construct: ORF TRCN0000474929
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000207.1_s317c1
- Derived from:
- ccsbBroadEn_03571
- DNA Barcode:
- CCATCCTTACCAGAAACAACTCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UBE2W (55284)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474929
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55284 | UBE2W | ubiquitin conjugating enzym... | NM_018299.6 | 100% | 100% | |
| 2 | human | 55284 | UBE2W | ubiquitin conjugating enzym... | NM_001271015.3 | 94.5% | 92.4% | (many diffs) |
| 3 | human | 55284 | UBE2W | ubiquitin conjugating enzym... | NM_001001481.4 | 93.2% | 93.2% | 16_48del |
| 4 | human | 55284 | UBE2W | ubiquitin conjugating enzym... | NR_073121.3 | 4.1% | 1_40del;147_148ins103;391_8276del | |
| 5 | human | 55284 | UBE2W | ubiquitin conjugating enzym... | NR_073120.3 | 4.1% | (many diffs) | |
| 6 | human | 55284 | UBE2W | ubiquitin conjugating enzym... | NR_073119.3 | 4% | (many diffs) | |
| 7 | mouse | 66799 | Ube2w | ubiquitin-conjugating enzym... | NM_025773.3 | 79.2% | 83.3% | (many diffs) |
| 8 | mouse | 66799 | Ube2w | ubiquitin-conjugating enzym... | XM_006495557.3 | 69.7% | 64.7% | (many diffs) |
| 9 | mouse | 66799 | Ube2w | ubiquitin-conjugating enzym... | XM_017322047.1 | 64.8% | 67.7% | (many diffs) |
| 10 | mouse | 66799 | Ube2w | ubiquitin-conjugating enzym... | NM_001271017.1 | 62.6% | 38.7% | (many diffs) |
| 11 | mouse | 66799 | Ube2w | ubiquitin-conjugating enzym... | NM_001271016.1 | 55.6% | 38.9% | (many diffs) |
| 12 | mouse | 66799 | Ube2w | ubiquitin-conjugating enzym... | XM_011238402.2 | 50.9% | 44.5% | (many diffs) |
| 13 | mouse | 66799 | Ube2w | ubiquitin-conjugating enzym... | XM_017322048.1 | 49.8% | 43.9% | (many diffs) |
| 14 | mouse | 66799 | Ube2w | ubiquitin-conjugating enzym... | NR_073122.1 | 10.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 91
- ORF end:
- 544
- ORF length:
- 453
- Sequence:
-
1 CTCAGACAGT GGTTCAAAGT TTTTTTCTTC CATTTCAGGT GTCGTGAGGC TAGCATCGAT 61 TGATCAACAA GTTTGTACAA AAAAGTTGGC ATGGCGTCAA TGCAGAAACG ACTACAGAAA 121 GAACTGTTGG CTTTGCAAAA TGACCCACCT CCTGGAATGA CCTTAAATGA GAAGAGTGTT 181 CAAAATTCAA TTACACAGTG GATTGTAGAC ATGGAAGGTG CACCAGGTAC CTTATATGAA 241 GGGGAAAAAT TTCAACTTCT ATTTAAATTT AGTAGTCGAT ATCCTTTTGA CTCTCCTCAG 301 GTCATGTTTA CTGGTGAAAA TATTCCTGTT CATCCTCATG TTTATAGCAA TGGTCATATC 361 TGTTTATCCA TTCTAACAGA AGACTGGTCC CCAGCGCTCT CAGTCCAATC AGTTTGTCTT 421 AGCATTATTA GCATGCTTTC CAGCTGCAAG GAAAAGAGAC GACCACCGGA TAATTCTTTT 481 TATGTGCGAA CATGTAACAA GAATCCAAAG AAAACAAAAT GGTGGTATCA TGATGATACT 541 TGTTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC 601 CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT 661 TGGCTTTATA TATCTTGTGG AAAGGACGAC CATCCTTACC AGAAACAACT CCCACGCGTT 721 AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag att