Construct: ORF TRCN0000474933
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014353.1_s317c1
- Derived from:
- ccsbBroadEn_12378
- DNA Barcode:
- CAACAAAGGCGTAACACGGGGACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LRCH2 (57631)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474933
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57631 | LRCH2 | leucine rich repeats and ca... | NM_001243963.1 | 99.9% | 99.8% | 2078G>A |
| 2 | human | 57631 | LRCH2 | leucine rich repeats and ca... | NM_020871.4 | 97.7% | 97.6% | 1737_1787del;2129G>A |
| 3 | human | 57631 | LRCH2 | leucine rich repeats and ca... | XM_006724724.3 | 96.8% | 96.6% | 1177_1178ins21;1716_1766del;2108G>A |
| 4 | human | 57631 | LRCH2 | leucine rich repeats and ca... | XM_017029696.2 | 76.7% | 74.7% | (many diffs) |
| 5 | mouse | 210297 | Lrch2 | leucine-rich repeats and ca... | XM_006528790.3 | 89.4% | 92.3% | (many diffs) |
| 6 | mouse | 210297 | Lrch2 | leucine-rich repeats and ca... | NM_001081173.1 | 87.5% | 90.2% | (many diffs) |
| 7 | mouse | 210297 | Lrch2 | leucine-rich repeats and ca... | XM_017318455.1 | 86.7% | 89.5% | (many diffs) |
| 8 | mouse | 210297 | Lrch2 | leucine-rich repeats and ca... | XM_006528789.3 | 86.4% | 89.2% | (many diffs) |
| 9 | mouse | 210297 | Lrch2 | leucine-rich repeats and ca... | XM_006528791.3 | 84.9% | 87.5% | (many diffs) |
| 10 | mouse | 210297 | Lrch2 | leucine-rich repeats and ca... | XM_006528788.3 | 84.6% | 87.3% | (many diffs) |
| 11 | mouse | 210297 | Lrch2 | leucine-rich repeats and ca... | XM_017318456.1 | 74% | 76.8% | (many diffs) |
| 12 | mouse | 210297 | Lrch2 | leucine-rich repeats and ca... | XM_006528792.3 | 72.6% | 75.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2313
- ORF length:
- 2244
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcggcgagt cagggaggag gcggtaacag tgggggcggc ggttgtggtg 121 gaggtggaag tagcggtggc tgtggcacgg ctggaggggg aggtggcggg gctggagggg 181 gaggcggcgg cggcggcggg accctggtgg tccccatccc ggtaccgact cttttcggtc 241 agccgttccc caacgggccg ccgtggaacc cggggagcct gcagcctcag cacaccgtga 301 ggagcctgga ccgggccctg gaagaggcgg gcagctccgg catcctgagc ctcagtggtc 361 ggaaactccg agacttcccg ggcagcggct acgacctgac ggacaccacc caagcagatc 421 tttccagaaa tcgttttaca gaaattcctt ctgatgtctg gttatttgca ccccttgaaa 481 cattaaattt atatcataat tgcatcaaaa ccattcctga agccattaaa aatctgcaga 541 tgttaacata ccttaacatt agccgaaatc ttttatcaac attgccaaaa tacctatttg 601 atcttcccct taaagttttg gtcgtcagta ataataaact ggtatccatt ccagaagaaa 661 ttgggaagtt aaaagattta atggaattgg atattagctg caatgagatt caagtccttc 721 cccaacaaat gggaaaatta cattcactta gagagctaaa tataagaaga aataatcttc 781 atgttttgcc agatgaatta ggagaccttc ccttagtcaa gctggatttc tcttgtaata 841 aagtgaccga aattccagtt tgttacagaa agctgcatca tttacaagta ataattttgg 901 ataacaatcc attgcaagta ccaccagcac agatatgttt aaagggtaag gtgcacatat 961 ttaagtactt aaatattcaa gcatgttgca gaatggataa gaaaccagat tccctggatc 1021 ttccatcatt gagtaaacga atgccctcac agcctctcac agacagtatg gaagattttt 1081 atccaaataa aaatcatggc cctgactctg gaattggaag tgataatgga gagaaacgat 1141 tatccacaac agaaccatca gatgatgaca cagtcagcct tcattctcaa gtctcagaat 1201 caaatagaga gcagacatca agaaatgaca gtcacataat aggaagtaag actgattctc 1261 agaaagacca ggaggtatat gattttgttg accccaacac agaagatgta gcagttcctg 1321 aacagggaaa tgcacatatt ggatcatttg tatcattctt taagggaaaa gaaaaatgtt 1381 ctgaaaaatc tcggaaaaat gaagaattag gagatgaaaa aagacttgag aaagaacagt 1441 tactggcaga ggaagaggat gatgatttga aggaagtaac tgatttgagg aaaatagctg 1501 ctcagttatt gcagcaagaa cagaagaaca ggattcttaa tcattcaact tctgtgatga 1561 gaaacaagcc aaaacaaact gtggaatgtg aaaagagtgt ctcagcagat gaagttaatt 1621 caccattatc acccctcacc tggcagccct tagaaaatca gaaggatcaa atagatgaac 1681 aaccgtggcc agaatctcac cctataatct ggcagagtga agaaaggagg cggagcaaac 1741 agattagaaa agaatatttc aagtataaat caatgaggaa gagttcaagt ggcaatgaaa 1801 atgatgagga atatgacaga actgatggtt tttcacacag tccctttggc ttgaagccta 1861 gatcagcttt tagccgctca tctcgccaag aatatggggc agcagatcca ggatttacaa 1921 tgagaagaaa gatggaacat ttacgggaag agcgagagca aatacgacaa cttcgcaaca 1981 atcttgaatc caggttaaaa gtaattttgc ctgatgacat tggagctgca ctgatggatg 2041 gggttGTTCT TTGCCATTTA GCCAATCATA TAAGGCCACG TTCTGTTGCT AGTATTCATG 2101 TACCATCACC AGCAGTGCCC AAACTGAGCA TGGCAAAATG TCGAAAAAAT GTAGAAAATT 2161 TTCTTGATGC TTGTAAAAAG TTGGGTGTCT CACAGGAAAG ACTTTGTTTG CCTCATCATA 2221 TTTTGGAAGA ACGAGGACTT GTGAAAGTTG GTGTCACAGT TCAGGCGCTC CTTGAATTAC 2281 CAACAACCAA GGCATCTCAG CTTTCTGTGG CTTTGCCAAC TTTCTTGTAC AAAGTGGTTG 2341 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 2401 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACA 2461 ACAAAGGCGT AACACGGGGA CCACGCGTTA AGTCgacaat caacctctgg attacaaaat 2521 ttgtgaaaga tt