Construct: ORF TRCN0000474956
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012330.1_s317c1
- Derived from:
- ccsbBroadEn_07779
- DNA Barcode:
- GTCTTAACGAATCCCCGAGTCTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SEC63 (11231)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474956
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11231 | SEC63 | SEC63 homolog, protein tran... | NM_007214.5 | 99.9% | 99.8% | 564C>T;1666G>A |
| 2 | human | 11231 | SEC63 | SEC63 homolog, protein tran... | XM_017010218.2 | 51.7% | 51.7% | 0_1ins1098;568G>A |
| 3 | mouse | 140740 | Sec63 | SEC63-like (S. cerevisiae) | NM_153055.3 | 91.4% | 96.1% | (many diffs) |
| 4 | mouse | 140740 | Sec63 | SEC63-like (S. cerevisiae) | XM_011243116.1 | 91.3% | 96% | (many diffs) |
| 5 | mouse | 140740 | Sec63 | SEC63-like (S. cerevisiae) | XM_017313800.1 | 74.1% | 77.7% | (many diffs) |
| 6 | mouse | 140740 | Sec63 | SEC63-like (S. cerevisiae) | XM_017313801.1 | 46.2% | 48.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2346
- ORF length:
- 2280
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cgggcagcag ttccagtacg atgacagtgg gaacaccttc ttctacttcc 121 tcacctcctt cgtggggctc atcgtgatcc cggcgacata ctacctctgg ccccgagatc 181 agaatgccga gcaaattcga ttaaagaata tcagaaaagt atatggaagg tgtatgtggt 241 atcgtttacg gttattaaaa ccccagccaa atattattcc tacagtaaag aaaatagttc 301 tgcttgcagg atgggcattg ttcttattcc ttgcatataa agtttccaaa acagaccgag 361 aataccaaga atacaatcct tatgaagtat taaatttgga tcctggagcc acagtagcag 421 aaattaaaaa acaatatcgt ttgctgtcac ttaaatatca tccagataaa ggaggtgatg 481 aggttatgtt catgaggata gcaaaagctt atgctgcttt aacggatgaa gagtcccgga 541 aaaattggga agaatttgga aatccagatg ggcctcaagc cacaagcttt ggaattgccc 601 tgccagcttg gatagttgac cagaaaaatt caattctggt tttacttgta tatggattgg 661 catttatggt tatccttcca gttgttgtgg gctcttggtg gtatcgctca atacgctata 721 gtggagacca gattctaata cgcacaacac agatttatac atactttgtt tataaaaccc 781 gaaatatgga tatgaaacgt cttatcatgg ttttggctgg agcttctgaa tttgatcctc 841 agtataataa agatgccaca agcagaccaa cggataatat tctaatacca cagctaatca 901 gagaaattgg cagcattaat ttaaagaaga atgagcctcc acttacctgc ccatatagcc 961 tgaaggccag agttctttta ctgtctcatc ttgctagaat gaaaattcct gagacccttg 1021 aagaagatca gcaattcatg ctaaaaaagt gtcctgccct acttcaagaa atggttaatg 1081 taatctgcca actaatagta atggcccgga accgtgaaga aagggagttt cgtgctccaa 1141 ctttggcatc cctagaaaac tgcatgaagc tttctcagat ggccgttcag ggacttcagc 1201 aatttaagtc tccccttctg cagctccctc atattgaaga ggacaatctt agacgggttt 1261 ctaatcataa gaagtataaa attaaaacta tccaggattt ggtgagttta aaagaatcag 1321 atcgtcacac tctactgcac ttccttgaag atgaaaaata tgaagaggtt atggctgtcc 1381 ttgggagttt tccatatgtg accatggata taaaatcaca ggtgttagat gatgaagata 1441 gcaacaacat cacagtagga tccttagtta cagtgttggt taagttgaca aggcaaacaa 1501 tggctgaagt atttgaaaag gagcagtcca tctgtgctgc agaggaacag ccagcagaag 1561 atgggcaggg tgaaactaac aagaacagga caaaaggagg atggcaacag aagagtaaag 1621 gacccaagaa aactgctaaa tcaaaaaaaa agaaaccttt aaaaaaaaaa cctacacctg 1681 tgctattacc acagtcaaag caacagaaac aaaagcaggc aaatggagtc attgggaatg 1741 aagctgcagt aaaggaagat gaagaagaag tttcagataa gggcagtgat tctgaagaag 1801 aagaaaccaa tagagattcc caaagtgaga aagatgatgg tagtgacaga gactctgata 1861 gagagcaaga tgaaaaacaa aacaaagatg atgaagcaga gtggcaagaa ttacaacaaa 1921 gcatacagcg aaaagagaga gctctattgg aaaccaaatc aaaaataaca catcctgtgt 1981 atagccttta ctttccTGAG GAAAAACAAG AATGGTGGTG GCTTTACATT GCAGATAGGA 2041 AGGAGCAGAC ATTAATATCC ATGCCATATC ATGTGTGTAC GCTGAAAGAT ACAGAGGAGG 2101 TAGAGCTGAA GTTTCCTGCA CCAGGCAAGC CTGGAAATTA TCAGTATACT GTGTTTCTGA 2161 GATCAGACTC CTATATGGGT TTGGATCAGA TTAAACCATT GAAGTTGGAA GTTCATGAGG 2221 CTAAGCCTGT GCCAGAAAAT CACCCACAGT GGGATACAGC AATAGAGGGG GATGAAGACC 2281 AGGAGGACAG TGAGGGCTTT GAAGATAGCT TTGAGGAAGA AGAGGAGGAA GAAGAAGATG 2341 ATGACTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 2401 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 2461 CTTGGCTTTA TATATCTTGT GGAAAGGACG AGTCTTAACG AATCCCCGAG TCTTCACGCG 2521 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt