Transcript: Human NM_007214.5

Homo sapiens SEC63 homolog, protein translocation regulator (SEC63), mRNA.

Source:
NCBI, updated 2019-08-22
Taxon:
Homo sapiens (human)
Gene:
SEC63 (11231)
Length:
6430
CDS:
200..2482

Additional Resources:

NCBI RefSeq record:
NM_007214.5
NBCI Gene record:
SEC63 (11231)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_007214.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000336301 TGGTATCGTTTACGGTTATTA pLKO_005 371 CDS 100% 15.000 21.000 N Sec63 n/a
2 TRCN0000129911 GCTTTACATTGCAGATAGGAA pLKO.1 2155 CDS 100% 3.000 4.200 N SEC63 n/a
3 TRCN0000147374 GTGGTATCGTTTACGGTTATT pLKO.1 370 CDS 100% 13.200 10.560 N SEC63 n/a
4 TRCN0000292323 GTGGTATCGTTTACGGTTATT pLKO_005 370 CDS 100% 13.200 10.560 N SEC63 n/a
5 TRCN0000150279 CCGAGCAAATTCGATTAAAGA pLKO.1 321 CDS 100% 5.625 4.500 N SEC63 n/a
6 TRCN0000129642 CCACAGCTAATCAGAGAAATT pLKO.1 1022 CDS 100% 13.200 9.240 N SEC63 n/a
7 TRCN0000147847 GCAAACAATGGCTGAAGTATT pLKO.1 1627 CDS 100% 13.200 9.240 N SEC63 n/a
8 TRCN0000147202 CCATTGAAGTTGGAAGTTCAT pLKO.1 2330 CDS 100% 4.950 3.465 N SEC63 n/a
9 TRCN0000297974 CCATTGAAGTTGGAAGTTCAT pLKO_005 2330 CDS 100% 4.950 3.465 N SEC63 n/a
10 TRCN0000149180 CCCTTGAAGAAGATCAGCAAT pLKO.1 1149 CDS 100% 4.950 3.465 N SEC63 n/a
11 TRCN0000292326 CCCTTGAAGAAGATCAGCAAT pLKO_005 1149 CDS 100% 4.950 3.465 N SEC63 n/a
12 TRCN0000128016 GCAAATGGAGTCGTTGGGAAT pLKO.1 1853 CDS 100% 4.050 2.835 N SEC63 n/a
13 TRCN0000292325 GCAAATGGAGTCGTTGGGAAT pLKO_005 1853 CDS 100% 4.050 2.835 N SEC63 n/a
14 TRCN0000149916 GCCCTACTTCAAGAAATGGTT pLKO.1 1190 CDS 100% 3.000 2.100 N SEC63 n/a
15 TRCN0000292324 GCCCTACTTCAAGAAATGGTT pLKO_005 1190 CDS 100% 3.000 2.100 N SEC63 n/a
16 TRCN0000128869 GATAGGAAGGAGCAGACATTA pLKO.1 2168 CDS 100% 13.200 7.920 N SEC63 n/a
17 TRCN0000146839 CAAGCCTGGAAATTATCAGTA pLKO.1 2260 CDS 100% 4.950 2.970 N SEC63 n/a
18 TRCN0000175765 GCAACAACATCACAGTAGGAT pLKO.1 1575 CDS 100% 3.000 1.800 N Sec63 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_007214.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07778 pDONR223 100% 99.9% 100% None 1251A>G n/a
2 TRCN0000478481 TTTCATACATCCCTGAGACGTCTA pLX_317 15.6% 99.9% 100% V5 1251A>G n/a
3 ccsbBroadEn_07779 pDONR223 100% 99.9% 99.8% None 564C>T;1666G>A n/a
4 ccsbBroad304_07779 pLX_304 0% 99.9% 99.8% V5 564C>T;1666G>A n/a
5 TRCN0000474956 GTCTTAACGAATCCCCGAGTCTTC pLX_317 15.6% 99.9% 99.8% V5 564C>T;1666G>A n/a
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