Construct: ORF TRCN0000474962
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005764.1_s317c1
- Derived from:
- ccsbBroadEn_14389
- DNA Barcode:
- CCTTGCCCGCTCACACCCGCCATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PSMA8 (143471)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474962
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 143471 | PSMA8 | proteasome 20S subunit alpha 8 | NM_144662.3 | 94.5% | 94.5% | 727_768del |
| 2 | human | 143471 | PSMA8 | proteasome 20S subunit alpha 8 | NM_001025096.2 | 92.1% | 92.1% | 227_228insAGTTTTGACAATTTTTAT;709_750del |
| 3 | human | 143471 | PSMA8 | proteasome 20S subunit alpha 8 | XM_011525818.1 | 83.8% | 82% | (many diffs) |
| 4 | human | 143471 | PSMA8 | proteasome 20S subunit alpha 8 | NM_001308188.2 | 81.5% | 79.6% | (many diffs) |
| 5 | human | 143471 | PSMA8 | proteasome 20S subunit alpha 8 | NM_001025097.2 | 77.3% | 77.3% | 113_114ins132;595_636del |
| 6 | mouse | 73677 | Psma8 | proteasome (prosome, macrop... | NM_001163609.1 | 81.9% | 88.2% | (many diffs) |
| 7 | mouse | 73677 | Psma8 | proteasome (prosome, macrop... | XM_006526293.2 | 67.9% | 73.8% | (many diffs) |
| 8 | mouse | 73677 | Psma8 | proteasome (prosome, macrop... | XM_006526294.3 | 56.6% | 60.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 792
- ORF length:
- 726
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gtctcgatat gacagggcga tcactgtctt ctccccagac ggacaccttt 121 ttcaagttga atatgcccag gaagcggtga agaaaggatc caccgcggtc ggaattcgag 181 gtaccaatat agttgttctt ggggtagaaa aaaaatctgt tgccaagctt caagatgaaa 241 gaactgtgag gaaaatttgt gcccttgatg accatgTCTG CATGGCTTTT GCAGTTTTGA 301 CAATTTTTAT AGGACTTACT GCTGATGCTA GAGTAGTAAT AAACAGAGCC CGTGTGGAGT 361 GCCAGAGCCA TAAGCTTACG GTTGAGGACC CAGTCACTGT AGAATACATA ACTCGCTTCA 421 TAGCAACTTT AAAGCAGAAA TATACCCAAA GCAATGGACG AAGACCTTTT GGTATTTCTG 481 CCTTAATTGT AGGTTTTGAT GATGATGGTA TCTCAAGATT GTATCAGACA GATCCTTCTG 541 GTACTTATCA TGCTTGGAAG GCAAATGCAA TAGGCCGAAG TGCTAAAACT GTTCGAGAAT 601 TTCTAGAAAA GAATTACACA GAAGATGCCA TAGCAAGTGA CAGTGAAGCT ATCAAGTTAG 661 CAATAAAAGC TTTGCTAGAA GTTGTCCAGT CTGGTGGAAA AAACATTGAA CTTGCTATAA 721 TAAGAAGAAA TCAACCTTTG AAGATGTTTA GTGCAAAAGA AGTTGAATTA TATGTAACTG 781 AAATAGAAAA GTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 841 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 901 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACCT TGCCCGCTCA CACCCGCCAT 961 TACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t