Construct: ORF TRCN0000474972
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017046.1_s317c1
- Derived from:
- ccsbBroadEn_10313
- DNA Barcode:
- TTCGTCAGGGGCCAAGACTACACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SBDSP1 (155370)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474972
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51119 | SBDS | SBDS ribosome maturation fa... | NM_016038.4 | 23.6% | 23.2% | (many diffs) |
| 2 | human | 155370 | SBDSP1 | SBDS pseudogene 1 | NR_024109.1 | 19.1% | (many diffs) | |
| 3 | human | 155370 | SBDSP1 | SBDS pseudogene 1 | NR_001588.2 | 18.4% | 1_283del;467_992del | |
| 4 | human | 155370 | SBDSP1 | SBDS pseudogene 1 | NR_024110.1 | 10.5% | 1_283del;467_1734del | |
| 5 | human | 155370 | SBDSP1 | SBDS pseudogene 1 | NR_024111.1 | 10.3% | (many diffs) | |
| 6 | mouse | 66711 | Sbds | SBDS ribosome assembly guan... | NM_023248.2 | 22.8% | 22.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 279
- ORF end:
- 462
- ORF length:
- 183
- Sequence:
-
1 GTCCAGGCAC CTCGATTAGT TCTCGAGCTT TTGGAGTACG TCGTCTTTAG GTTGGGGGGA 61 GGGGTTTTAT GCGATGGAGT TTCCCCACAC TGAGTGGGTG GAGACTGAAG TTAGGCCAGC 121 TTGGCACTTG ATGTAATTCT CCTTGGAATT TGCCCTTTTT GAGTTTGGAT CTTGGTTCAT 181 TCTCAAGCCT CAGACAGTGG TTCAAAGTTT TTTTCTTCCA TTTCAGGTGT CGTGAGGCTA 241 GCATCGATTG ATCAACAAGT TTGTACAAAA AAGTTGGCAT GTCGATCTTC ACCCCCACCA 301 ACCAGATCCG CCTAACCAAT GTGGCCGTGG TACGGATGAA GCGCGCCAGG AAGCGCTTCG 361 AAATCGCCTG CTACAGAAAC AAGGTCGTCG GCTGGCGGAG CGGCTTGGAA AAAGACCTTG 421 ATGAAGTTCT GCAGACCCAC TCAGTGTTTG TAAATGTTTC CTACCCAACT TTCTTGTACA 481 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 541 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 601 AGGACGATTC GTCAGGGGCC AAGACTACAC CACGCGTTAA GTCgacaatc aacctctgga 661 ttacaaaatt tgtgaaagat t