Construct: ORF TRCN0000474974
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009605.1_s317c1
- Derived from:
- ccsbBroadEn_07924
- DNA Barcode:
- GAGAGTTTCTCCAGGGAGCGCTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZKSCAN5 (23660)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474974
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23660 | ZKSCAN5 | zinc finger with KRAB and S... | NM_001318082.2 | 99.9% | 99.8% | 2377A>G |
| 2 | human | 23660 | ZKSCAN5 | zinc finger with KRAB and S... | NM_014569.4 | 99.9% | 99.8% | 2377A>G |
| 3 | human | 23660 | ZKSCAN5 | zinc finger with KRAB and S... | NM_145102.4 | 99.9% | 99.8% | 2377A>G |
| 4 | human | 23660 | ZKSCAN5 | zinc finger with KRAB and S... | XM_017011921.2 | 99.8% | 99.7% | 634_635insAGA;2374A>G |
| 5 | human | 23660 | ZKSCAN5 | zinc finger with KRAB and S... | XM_017011922.1 | 99.8% | 99.7% | 634_635insAGA;2374A>G |
| 6 | human | 23660 | ZKSCAN5 | zinc finger with KRAB and S... | NM_001318083.2 | 91.2% | 91.1% | 552_553ins219;2158A>G |
| 7 | human | 23660 | ZKSCAN5 | zinc finger with KRAB and S... | XM_017011924.1 | 91.2% | 91.1% | 552_553ins219;2158A>G |
| 8 | human | 23660 | ZKSCAN5 | zinc finger with KRAB and S... | XM_011515999.2 | 82.2% | 82.1% | 0_1ins447;1930A>G |
| 9 | human | 23660 | ZKSCAN5 | zinc finger with KRAB and S... | XM_017011925.1 | 82% | 82% | 0_1ins447;187_188insAGA;1927A>G |
| 10 | human | 23660 | ZKSCAN5 | zinc finger with KRAB and S... | NM_001318084.1 | 68.4% | 68.4% | 0_1ins792;1585A>G |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2583
- ORF length:
- 2517
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat aatgaccgaa tcccgagaag ttatagactt agacccccca gctgagactt 121 cccaggagca ggaagacctt ttcatagtga aggtggaaga agaagactgc acctggatgc 181 aggagtacaa cccgccaacg tttgagactt tttaccagcg cttcaggcac ttccagtacc 241 atgaggcttc aggaccccgg gaggctctca gccaactccg ggtgctctgc tgtgagtggc 301 tgaggcccga gctgcacacg aaggagcaga tcctggagct gctggtgctg gagcagttcc 361 tgaccatcct gcctgaagag ttccagccct gggtgaggga acatcaccct gaaagtggag 421 aagaggcggt ggccgtgata gaaaatatac agcgagaact tgaggaacgc agacagcaga 481 ttgttgcctg ccctgatgtg cttcctcgga agatggcaac acctggagca gtgcaggagt 541 cctgcagccc ccatcccctg accgtggaca cccagcctga gcaagcgcca cagaagcctc 601 gtctcctgga ggaaaatgcc cttcctgttc tccaagttcc ttcccttccc ctgaaggaca 661 gccaggagct gacagcttca cttctctcaa ctgggtccca gaagttggtg aaaattgaag 721 aggtggctga tgtggctgta tccttcatcc tggaggaatg ggggcatttg gaccagtccc 781 agaagtccct ttatagggat gacaggaagg agaactatgg gagtattact tccatgggtt 841 atgagtccag ggacaatatg gagctcatag tgaagcagat ttctgatgac tctgaatcac 901 actgggtggc gccagaacac accgaaagga gcgttcctca ggatccagac tttgcagaag 961 tcagtgacct taaaggcatg gtacaaaggt ggcaggtcaa ccccactgtg gggaaatcaa 1021 ggcagaatcc ttcccagaaa agggatctgg atgcaatcac agacatcagc cctaagcaaa 1081 gcacacatgg cgagagaggg cacagatgca gcgattgtgg caaattcttc ctccaagcct 1141 caaactttat tcagcatcgg cgcatccaca ctggagaaaa accgtttaag tgcggagaat 1201 gtgggaagag ctacaatcag cgggtgcacc tcacccagca ccagcgcgtc cacacagggg 1261 agaaacccta caaatgtcag gtgtgcggaa aggctttccg ggtgagttcc cacctggttc 1321 agcaccacag tgtccacagc ggagagaggc cctatggctg caatgagtgt gggaagaact 1381 tcggtcgcca ttcgcatctg atcgaacacc taaaacgcca cttcagggag aaatcccaga 1441 gatgcagtga caaaagaagt aagaacacaa aattaagtgt taagaagaaa atttcagaat 1501 attcagaagc agacatggaa ctatctggaa aaacccaaag aaatgtttct caagttcaag 1561 attttggaga aggctgtgag tttcaaggca agctggatag aaagcaggga attcccatga 1621 aagagatact aggacaacca tcttcaaaga ggatgaacta cagtgaagtc ccatatgtcc 1681 acaaaaaatc ctccactgga gagagaccac ataaatgtaa cgagtgtggg aaaagcttca 1741 ttcagagtgc acatcttatt caacatcaaa gaatacacac tggggagaaa ccattcaggt 1801 gtgaggaatg tgggaaaagc tacaaccaac gcgtgcacct aactcagcat cagcgcgtcc 1861 acacaggtga gaagccctac acctgtccct tatgtgggaa agccttcaga gtgaggtccc 1921 accttgttca gcatcagagc gtgcacagtg gggagagacc cttcaagtgt aacgaatgtg 1981 ggaaaggctt tgggaggcgt tcccacctgg ctggacatct tcgactccac tcccgagaga 2041 aatcccatca gtgtcGTGAA TGTGGGGAAA TCTTTTTTCA GTACGTTAGC CTAATTGAAC 2101 ATCAGGTGCT CCACATGGGT CAGAAAAATG AAAAAAATGG CATCTGTGAG GAAGCATATA 2161 GTTGGAACTT GACAGTGATT GAAGACAAGA AGATTGAGTT ACAAGAGCAG CCTTATCAGT 2221 GTGATATCTG TGGAAAAGCC TTTGGTTATA GCTCAGACCT CATTCAGCAT TACAGAACTC 2281 ATACAGCAGA GAAGCCCTAT CAATGTGATA TATGTAGAGA AAATGTTGGC CAGTGTTCCC 2341 ACACCAAACA ACATCAAAAA ATCTACTCCA GCACAAAATC CCATCAATGT CATGAATGTG 2401 GCAGAGGCTT CACTCTGAAG TCACATCTTA ATCAACATCA GGGAATCCAT ACTGGTGAGA 2461 AACCTTTTCA ATGTAAAGAA TGTGGAATGA ATTTCAGCTG GAGTTGTAGC CTCTTTAAAC 2521 ACCTGAGAAG CCATGAGAGG ACAGATCCCA TAAATACCTT AAGTGTAGAG GGGTCTCTGT 2581 TGTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 2641 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 2701 GGCTTTATAT ATCTTGTGGA AAGGACGAGA GAGTTTCTCC AGGGAGCGCT TCACGCGTTA 2761 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt