Transcript: Human NM_001318084.1

Homo sapiens zinc finger with KRAB and SCAN domains 5 (ZKSCAN5), transcript variant 5, mRNA.

Source:
NCBI, updated 2019-09-25
Taxon:
Homo sapiens (human)
Gene:
ZKSCAN5 (23660)
Length:
4014
CDS:
697..2424

Additional Resources:

NCBI RefSeq record:
NM_001318084.1
NBCI Gene record:
ZKSCAN5 (23660)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001318084.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000235855 GTTGTAGAATAGCTCTTAATT pLKO_005 2418 CDS 100% 15.000 21.000 N ZKSCAN5 n/a
2 TRCN0000235857 TTCAGTACGTTAGCCTAATTG pLKO_005 1916 CDS 100% 13.200 18.480 N ZKSCAN5 n/a
3 TRCN0000016401 CGCATCTGATCGAACACCTAA pLKO.1 1232 CDS 100% 4.950 6.930 N ZKSCAN5 n/a
4 TRCN0000235858 ATCCCATCAGTGTCGTGAATG pLKO_005 1881 CDS 100% 10.800 8.640 N ZKSCAN5 n/a
5 TRCN0000016399 GCCTCAAACTTTATTCAGCAT pLKO.1 976 CDS 100% 2.640 2.112 N ZKSCAN5 n/a
6 TRCN0000235856 GAGAGGACAGATCCCATAAAT pLKO_005 2374 CDS 100% 15.000 10.500 N ZKSCAN5 n/a
7 TRCN0000016402 GCTGGAGTTGTAGCCTCTTTA pLKO.1 2336 CDS 100% 13.200 9.240 N ZKSCAN5 n/a
8 TRCN0000016398 CCCACACCAAACAACATCAAA pLKO.1 2177 CDS 100% 5.625 3.938 N ZKSCAN5 n/a
9 TRCN0000016400 GCTGGATAGAAAGCAGGGAAT pLKO.1 1431 CDS 100% 4.050 2.835 N ZKSCAN5 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001318084.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07924 pDONR223 100% 68.4% 68.4% None 0_1ins792;1585A>G n/a
2 ccsbBroad304_07924 pLX_304 0% 68.4% 68.4% V5 0_1ins792;1585A>G n/a
3 TRCN0000474974 GAGAGTTTCTCCAGGGAGCGCTTC pLX_317 21.2% 68.4% 68.4% V5 0_1ins792;1585A>G n/a
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