Construct: ORF TRCN0000475014
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012021.1_s317c1
- Derived from:
- ccsbBroadEn_12021
- DNA Barcode:
- CCGGATTTTAACCCTTCACTGAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PANK1 (53354)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475014
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 53354 | PANK1 | pantothenate kinase 1 | XM_017016338.1 | 100% | 100% | |
| 2 | human | 53354 | PANK1 | pantothenate kinase 1 | NM_138316.4 | 42.1% | 33.3% | (many diffs) |
| 3 | human | 53354 | PANK1 | pantothenate kinase 1 | XM_017016337.1 | 38.2% | 38.2% | 1_663del |
| 4 | human | 53354 | PANK1 | pantothenate kinase 1 | XM_017016336.1 | 37.1% | 37.1% | 1_696del |
| 5 | human | 53354 | PANK1 | pantothenate kinase 1 | XM_017016335.1 | 36.9% | 36.9% | 1_702del |
| 6 | human | 53354 | PANK1 | pantothenate kinase 1 | XM_024448040.1 | 36.9% | 36.9% | 1_702del |
| 7 | human | 53354 | PANK1 | pantothenate kinase 1 | XM_024448041.1 | 36.9% | 36.9% | 1_702del |
| 8 | human | 53354 | PANK1 | pantothenate kinase 1 | NM_148978.3 | 36.7% | 36.7% | 1_708del |
| 9 | human | 53354 | PANK1 | pantothenate kinase 1 | XM_017016334.1 | 35.3% | 35.3% | 1_753del |
| 10 | human | 53354 | PANK1 | pantothenate kinase 1 | XM_017016333.2 | 34.2% | 34.2% | 1_789del |
| 11 | human | 53354 | PANK1 | pantothenate kinase 1 | NM_148977.2 | 22.9% | 22.9% | 1_1383del |
| 12 | mouse | 75735 | Pank1 | pantothenate kinase 1 | NM_023792.2 | 34.4% | 35.9% | (many diffs) |
| 13 | mouse | 75735 | Pank1 | pantothenate kinase 1 | XM_006527427.3 | 25.4% | 21% | (many diffs) |
| 14 | mouse | 75735 | Pank1 | pantothenate kinase 1 | NM_001114339.2 | 23.4% | 24.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 477
- ORF length:
- 411
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttG TACAAAAAAG 61 TTGGCATGGC AGCTAAAGGC GACAGCACCA ATGTTGATAA ACTGGTGAAG GACATTTACG 121 GAGGAGACTA TGAACGATTT GGCCTTCAAG GATCTGCTGT AGCATCAAGC TTTGGCAACA 181 TGATGAGTAA AGAAAAGCGA GATTCCATCA GCAAGGAAGA CCTCGCCCGG GCCACATTGG 241 TCACCATCAC CAACAACATT GGCTCCATTG CTCGGATGTG CGCGTTGAAT GAGAACATAG 301 ACAGAGTTGT GTTTGTTGGA AATTTTCTCA GAATCAATAT GGTCTCCATG AAGCTGCTGG 361 CATATGCCAT GGATTTTTGG TCCAAAGGAC AACTGAAAGC TCTGTTTTTG GAACATGAGG 421 GTTATTTTGG AGCCGTTGGG GCACTGTTGG AACTGTTCAA AATGACTGAT GACAAGTACC 481 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 541 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 601 ATATATCTTG TGGAAAGGAC GACCGGATTT TAACCCTTCA CTGAGCACGC GTTAAGTCga 661 caatcaacct ctggattaca aaatttgtga aagatt