Construct: ORF TRCN0000475020
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018268.1_s317c1
- Derived from:
- ccsbBroadEn_13447
- DNA Barcode:
- GGAGTATTTGACGGATTCCCAAGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STXBP4 (252983)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475020
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 252983 | STXBP4 | syntaxin binding protein 4 | NM_178509.6 | 95.9% | 95.6% | 274G>A;946_1011del;1513G>A |
| 2 | human | 252983 | STXBP4 | syntaxin binding protein 4 | XM_005257187.4 | 95.5% | 95.3% | (many diffs) |
| 3 | human | 252983 | STXBP4 | syntaxin binding protein 4 | XM_017024411.2 | 93.2% | 92.9% | (many diffs) |
| 4 | human | 252983 | STXBP4 | syntaxin binding protein 4 | XM_017024410.1 | 92.8% | 92.6% | (many diffs) |
| 5 | human | 252983 | STXBP4 | syntaxin binding protein 4 | XM_017024412.2 | 85.6% | 85.5% | 274G>A;946_1011del;1488_1489ins171 |
| 6 | human | 252983 | STXBP4 | syntaxin binding protein 4 | XM_017024413.1 | 79.3% | 79.1% | (many diffs) |
| 7 | human | 252983 | STXBP4 | syntaxin binding protein 4 | XM_006721798.4 | 59.9% | 59.1% | (many diffs) |
| 8 | human | 252983 | STXBP4 | syntaxin binding protein 4 | XM_006721797.4 | 59.6% | 58.9% | (many diffs) |
| 9 | human | 252983 | STXBP4 | syntaxin binding protein 4 | XM_017024414.2 | 57.9% | 57.1% | (many diffs) |
| 10 | human | 252983 | STXBP4 | syntaxin binding protein 4 | XM_017024415.1 | 57.9% | 57.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1662
- ORF length:
- 1593
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaataaaaat acatctactg tagtatcacc cagtctactt gaaaaggatc 121 ctgcctttca gatgattaca attgccaagg aaacaggcct tggcctgaag gtactaggag 181 gaattaaccg gaatgaaggc ccattggtat atattcagga aattattcct ggaggagact 241 gttataagga tggtcgtttg aagccaggag atcaacttgt ctcagtcaac aaggaatcta 301 tgattggtgt atcatttgaa gaagcaaaaa gcataattac cagagccaag ttgaggttag 361 aatctgcttg ggagatagca ttcataagac aaaaatccga caacattcag ccagaaaatc 421 tgtcatgtac atcacttata gaagcttcag gagaatatgg acctcaagcc tcaacattaa 481 gtcttttttc ttctcctcct gaaatactaa tcccaaagac ctcatccact cccaaaacaa 541 ataatgacat tttatcttct tgtgagataa aaactggata caacaaaaca gtacagattc 601 caattacttc agaaaacagt actgtgggtt tgtctaatac agatgttgct tctgcctgga 661 ctgaaaatta tgggctacaa gaaaagatct ccctaaatcc ctctgttcgc tttaaggcag 721 agaaactgga aatggctcta aattatcttg gtattcagcc cacaaaggaa caacaccaag 781 ccctgagaca gcaagtacaa gcagactcaa aagggacagt gtcttttgga gattttgtcc 841 aggttgccag aaacttgttt tgcttgcagt tggatgaagt aaatgttggt gcacatgaaa 901 tttccaatat attagattca cagcttcttc cttgtgattc ttcagaagca gatgaaatgg 961 aaaggctcaa gtgtgaaaga gatgatgcct tgaaagaagt aaatacactt aaggaagcca 1021 aagctgtagt tgaagaaaca agagccctgc gtagtcggat tcatcTTGCT GAAGCTGCTC 1081 AGAGACAGGC ACATGGAATG GAAATGGACT ATGAAGAAGT GATCCGTCTG TTAGAGGCCA 1141 AGATTACAGA GCTAAAGGCT CAGCTTGCTG ATTATTCTGA CCAAAATAAA GAAAGTGTTC 1201 AGGATTTAAA AAAGAGAATC ATGGTACTCG ACTGCCAATT ACGAAAATCA GAAATGGCTC 1261 GAAAAACTTT TGAGGCATCC ACTGAAAAGC TTCTTCATTT TGTAGAGGCT ATTCAAGAAG 1321 TATTTTCTGA TAATTCTACT CCTTTATCAA ATTTAAGTGA AAGAAGAGCT GTGTTAGCTT 1381 CTCAGACTTC CCTCACACCA CTGGGAAGGA ATGGACGTAG CATCCCAGCA ACGCTGGCGC 1441 TTGAATCTAA GGAACTTGTT AAATCTGTTC GTGCCTTACT TGATATGGAT TGTTTACCTT 1501 ATGGGTGGGA GGAAACTTAC ACAGCAGATG GAATCAAGTA CTTCATCAAT CATGTAACAC 1561 AGACTACATC CTGGATCCAT CCCGTGATGA GTGTCCTGAA TCTATCTCGC TCAGAGGAGA 1621 ATGAAGAGGA TTGCTCTAGA GAACTCCCCA ACCAGAAAAG TTTGCCAACT TTCTTGTACA 1681 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1741 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1801 AGGACGAGGA GTATTTGACG GATTCCCAAG GACGCGTTAA GTCgacaatc aacctctgga 1861 ttacaaaatt tgtgaaagat t