Construct: ORF TRCN0000475048
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010438.1_s317c1
- Derived from:
- ccsbBroadEn_11634
- DNA Barcode:
- GTCCGATGCATTTCAGATAGATCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- VASH1 (22846)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475048
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 22846 | VASH1 | vasohibin 1 | NM_014909.5 | 54.6% | 50.1% | (many diffs) |
| 2 | human | 22846 | VASH1 | vasohibin 1 | XR_001750190.1 | 7.6% | (many diffs) | |
| 3 | mouse | 238328 | Vash1 | vasohibin 1 | NM_177354.4 | 46.8% | 45% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 678
- ORF length:
- 612
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc aggggggaag aaggtggctg ggggtggcag cagcggtgcc actccaacgt 121 ccgctgcggc caccgccccc tctggggtca ggcgtttgga gaccagcgaa ggaacctcag 181 cccagagaga tgaggagcca gaagaggaag gggaagagga cctgcgagac ggaggcgtcc 241 ccttctttgt caaccggggt gggctacctg tggatgaggc cacctgggaa aggatgtgga 301 aacacgtggc caagatccac cccgatggag agaaggtggc gcaacggatc cgtggggcca 361 cagacctgcc caagatcccc ataccgagtg tgcctacgtt ccagccgtct acacctgtcc 421 ctgagcgcct ggaagctgtg cagcgctata tcagagagct gcagtacaat cacacaggga 481 cacagttctt tgaaattaag aagagcagac ctctgacagg gctgatggac ctggccaagg 541 aaatgaccaa agaggccctg ccaatcaaat GCCTGGAAGC CGTGATCCTG GGAATGTATC 601 CTTCCTCGCC TGAAGGGGAG GGGTCCGGGC TTCTCTGGGC CTCAGCTTCC TGCTCTGAAA 661 GTGAGGGTGG GGTAGGTTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 721 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 781 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGTCCGAT GCATTTCAGA 841 TAGATCTACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt