Construct: ORF TRCN0000475054
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018894.2_s317c1
- Derived from:
- ccsbBroadEn_15106
- DNA Barcode:
- CACTTACGCCACCACTATGTATCA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- SCYL2 (55681)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475054
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55681 | SCYL2 | SCY1 like pseudokinase 2 | NM_001317784.1 | 99.7% | 74.9% | (many diffs) |
| 2 | human | 55681 | SCYL2 | SCY1 like pseudokinase 2 | NM_017988.6 | 99.7% | 74.9% | (many diffs) |
| 3 | human | 55681 | SCYL2 | SCY1 like pseudokinase 2 | NM_001330253.1 | 99.2% | 74.5% | (many diffs) |
| 4 | human | 55681 | SCYL2 | SCY1 like pseudokinase 2 | NM_001330254.1 | 99.2% | 74.5% | (many diffs) |
| 5 | human | 55681 | SCYL2 | SCY1 like pseudokinase 2 | NM_001330256.1 | 80.7% | 56% | (many diffs) |
| 6 | mouse | 213326 | Scyl2 | SCY1-like 2 (S. cerevisiae) | NM_198021.2 | 88.6% | 71.7% | (many diffs) |
| 7 | mouse | 213326 | Scyl2 | SCY1-like 2 (S. cerevisiae) | NM_001310704.1 | 88.5% | 71.6% | (many diffs) |
| 8 | mouse | 213326 | Scyl2 | SCY1-like 2 (S. cerevisiae) | XM_011243394.2 | 88.2% | 71.4% | (many diffs) |
| 9 | mouse | 213326 | Scyl2 | SCY1-like 2 (S. cerevisiae) | XM_006513458.3 | 88.1% | 71.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2175
- ORF length:
- 2106
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagtccatg cttaataaat tgaagagtac tgttacaaaa gtaacagctg 121 atgtcactag tgctgtaatg ggaaatcctg tcactagaga atttgatgtt ggtcgacaca 181 ttgccagtgg tggcaatggg ctagcttgga agatttttaa tggcacaaaa aagtcaacaa 241 agcaggaagt ggcagttttt gtctttgata aaaaactgat tgacaagtat caaaaatttg 301 aaaaggatca aatcattgat tctctaaaac gaggagtcca acagttaact cggcttcgac 361 accctcgact tcttactgtc cagcatcctt tagaagaatc cagggattgc ttggcatttt 421 gtacagaacc agtttttgcc agtttagcca atgttcttgg taactgggaa aatctacctt 481 cccctatatc tccagacatt aaggattata aactttatga tgtagaaacc aaatatggtt 541 tgcttcaggt ttctgaagga ttgtcattct tgcatagcag tgtgaaaatg gtgcatggaa 601 atatcactcc tgaaaatata attttgaata aaagtggagc ctggaaaata atgggttttg 661 atttttgtgt atcatcaacc aatccttctg aacaagagcc taaatttcct tgtaaagaat 721 gggacccaaa tttaccttca ttgtgtcttc caaatcctga atatttggct cctgaataca 781 tactttctgt gagctgtgaa acagccagtg atatgtattc tttaggaact gttatgtatg 841 ctgtatttaa taaagggaaa cctatatttg aagtcaacaa gcaagatatt tacaagagtt 901 tcagtaggca gttggatcag ttgagtcgtt taggatctag ttcacttaca aatatacctg 961 aggaagttcg tgaacatgta aagctactgt taaatgtaac tccgactgta agaccagatg 1021 cagatcaaat gacaaagatt cccttctttg atgatgttgg tgcagtaaca ctgcaatatt 1081 ttgatacctt attccaaaga gataatcttc agaaatcaca gtttttcaaa ggactgccaa 1141 aggttctacc aaaactgccc aagcgtgtca ttgtgcagag aattttgcct tgtttgactt 1201 cagaatttgt aaaccctgac atggtacctt ttgttttgcc caatgttcta cttattgctg 1261 aggaatgcac caaagaagaa tatgtcaaat taattcttcc tgaacttggc cctgtgttta 1321 agcagcagga gccaatccag attttgttaa ttttcctaca aaaaatggat ttgctactaa 1381 ccaaaacccc tcctgatgag ataaagaaca gtgttctacc catggtttac agagcactag 1441 aagctccttc cattcagatc caggagctct gtctaaacat cattccaacc tttgcaaatc 1501 ttatagacta cccatccatg aaaaacgctt tgataccaag aattaaaaat gcttgtctac 1561 aaacatcttc ccttgcggtt cgtgtaaatt cattagtgtg cttaggaaag attttggaat 1621 acttggataa gtggtttgta cttgatgata tcctaccctt cttacaacaa attccatcca 1681 aggaacctgc ggtcctcatg ggaattttag gtatttacaa atgtactttt actcataaga 1741 agttgggaat caccaaagag cagctggccg gaaaagtgtt gcctcatctt attcccctga 1801 gtattgaaaa caatcttaat cttaatcagt tcaattcttt catttccgtc ataaaagaaa 1861 tgcttaatag attggagtct gaacataaga ctaaactgga gcaacttcat ataatgcaag 1921 aacagcagaa atctttggat ataggaaatc aaatgaatgt ttctgaggag atgaaagtta 1981 caaatattgg gaatcagcaa attgacaaag tttttaacaa cattggagca gaccttctga 2041 ctggcagtga gtccgaaaat aaagaggacg ggtaccggaa taaccataaa agagcatcac 2101 ttacacctga agaaaaacaa aaattagcaa aagaacaaga gcaggcacag aagctgaaaa 2161 gcccagcagc ctcttaaacc cccaagtgca cacacctgtt gctactgtta aacagactaa 2221 ggacttgaca gacacattga tggataatat gtcatccttg accagccttt ctgttagtac 2281 ccctaaatct tctgcttcaa gtactttcac ttctgttcct tccatgggca ttggtatgat 2341 gttttctaca ccaactgata atacaaagag aaatttgaca aatggcctaa atGCCAATAT 2401 GGGCTTTCAG ACTTCAGGAT TCAACATGCC CGTTAATACA AACCAGAACT TCTACAGTAG 2461 TCCAAGCACA GTTGGAGTGA CCAAGATGAC TCTGGGAACA CCTCCCACTT TGCCAAACTT 2521 CAATGCTTTG AGTGTTCCTC CTGCTGGTGC AAAGCAGACC CAACAAAGAC CCACAGATAT 2581 GTCTGCCCTT AATAATCTCT TTGGCCCTCA GAAACCCAAA GTTAGCATGA ACCAGTTATC 2641 ACAACAGAAA CCAAATCAGT GGCTTAATCA GTTTGTACCT CCTCAAGGTT CTCCAACTAT 2701 GGGCAGTTCA GTAATGGGGA CACAGATGAA CGTGATAGGA CAATCTGCTT TTGGTATGCA 2761 GGGTAATCCT TTCTTTAACC CACAGAACTT TGCACAGCCA CCAACTACTA TGACCAATAG 2821 CAGTTCAGCT AGCAATGATT TAAAAGATCT TTTTGGGTTG CCAACTTTCT TGTACAAAGT 2881 GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG 2941 AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA 3001 CGACACTTAC GCCACCACTA TGTATCAACG CGTTAAGTCg acaatcaacc tctggattac 3061 aaaatttgtg aaagatt