Construct: ORF TRCN0000475058
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002887.1_s317c1
- Derived from:
- ccsbBroadEn_04882
- DNA Barcode:
- CGATATTCTATAAACCACCCGGAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ENPP6 (133121)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475058
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 133121 | ENPP6 | ectonucleotide pyrophosphat... | NM_153343.4 | 100% | 100% | |
| 2 | mouse | 320981 | Enpp6 | ectonucleotide pyrophosphat... | NM_177304.4 | 84% | 86.1% | (many diffs) |
| 3 | mouse | 320981 | Enpp6 | ectonucleotide pyrophosphat... | XM_017312861.1 | 72.3% | 74.5% | (many diffs) |
| 4 | mouse | 320981 | Enpp6 | ectonucleotide pyrophosphat... | XM_017312862.1 | 72.3% | 74.5% | (many diffs) |
| 5 | mouse | 320981 | Enpp6 | ectonucleotide pyrophosphat... | XM_006509444.3 | 72.3% | 70.8% | (many diffs) |
| 6 | mouse | 320981 | Enpp6 | ectonucleotide pyrophosphat... | XM_011242227.2 | 72.3% | 70.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1386
- ORF length:
- 1320
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc agtgaagctt gggaccctcc tgctggccct tgccctgggc ctggcccagc 121 cagcctctgc ccgccggaag ctgctggtgt ttctgctgga tggttttcgc tcagactaca 181 tcagtgatga ggcgctggag tcattgcctg gtttcaaaga gattgtgagc aggggagtaa 241 aagtggatta cttgactcca gacttcccta gtctctcgta tcccaattat tataccctaa 301 tgactggccg ccattgtgaa gtccatcaga tgatcgggaa ctacatgtgg gaccccacca 361 ccaacaagtc ctttgacatt ggcgtcaaca aagacagcct aatgcctctc tggtggaatg 421 gatcagaacc tctgtgggtc actctgacca aggccaaaag gaaggtctac atgtactact 481 ggccaggctg tgaggttgag attctgggtg tcagacccac ctactgccta gaatataaaa 541 atgtcccaac ggatatcaat tttgccaatg cagtcagcga tgctcttgac tccttcaaga 601 gtggccgggc cgacctggca gccatatacc atgagcgcat tgacgtggaa ggccaccact 661 acgggcctgc atctccgcag aggaaagatg ccctcaaggc tgtagacact gtcctgaagt 721 acatgaccaa gtggatccag gagcggggcc tgcaggaccg cctgaacgtc attattttct 781 cggatcacgg aatgaccgac attttctgga tggacaaagt gattgagctg aataagtaca 841 tcagcctgaa tgacctgcag caagtgaagg accgcgggcc tgttgtgagc ctttggccgg 901 cccctgggaa acactctgag atatataaca aactgagcac agtggaacac atgactgtct 961 acgagaaaga agccatccca agcaggttct attacaagaa aggaaagttt gtctctcctt 1021 tgactTTAGT GGCTGATGAA GGCTGGTTCA TAACTGAGAA TCGAGAGATG CTTCCGTTTT 1081 GGATGAACAG CACCGGCAGG CGGGAAGGTT GGCAGCGTGG ATGGCACGGC TACGACAACG 1141 AGCTCATGGA CATGCGGGGC ATCTTCCTGG CCTTCGGACC TGATTTCAAA TCCAACTTCA 1201 GAGCTGCTCC TATCAGGTCG GTGGACGTCT ACAATGTCAT GTGCAATGTG GTGGGCATCA 1261 CCCCGCTGCC CAACAACGGA TCCTGGTCCA GGGTGATGTG CATGCTGAAG GGCCGCGCCA 1321 GCACTGCCCC GCCTGTCTGG CCCAGCCACT GTGCCCTGGC ACTGATTCTT CTCTTCCTGC 1381 TTGCATACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1441 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1501 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACGATATTCT ATAAACCACC CGGAGACGCG 1561 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt