Construct: ORF TRCN0000475068
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018065.1_s317c1
- Derived from:
- ccsbBroadEn_06013
- DNA Barcode:
- CCAGGTAATGCTACACACGGGTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CLPTM1 (1209)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475068
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1209 | CLPTM1 | CLPTM1 regulator of GABA ty... | NM_001294.4 | 96.7% | 96% | (many diffs) |
| 2 | human | 1209 | CLPTM1 | CLPTM1 regulator of GABA ty... | NM_001282175.1 | 93.8% | 92.5% | (many diffs) |
| 3 | human | 1209 | CLPTM1 | CLPTM1 regulator of GABA ty... | NM_001282176.1 | 81.9% | 81.1% | (many diffs) |
| 4 | mouse | 56457 | Clptm1 | cleft lip and palate associ... | NM_019649.2 | 85.9% | 91.1% | (many diffs) |
| 5 | mouse | 56457 | Clptm1 | cleft lip and palate associ... | XM_006540227.1 | 72.2% | 76.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2124
- ORF length:
- 2058
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcggcgcag gaggcggacg gggcccgcag cgccgtggtg gcggccgggg 121 gaggcagctc cggtcaggtg accagcaatg gcagcatcgg gagggacccg ccagcggaga 181 cccagcctca gaacccaccg gcccagccgg cacccaatgc ctggcaggtc atcaaaggtg 241 tgctgtttag gatcttcatc atctgggcca tcagcagttg gttccgccga gggccggccc 301 ctcaggacca ggcgggcccc ggaggagccc cacgcgtcgc cagccgcaac ctgttcccca 361 aagacacttt aatgaacctg catgtgtaca tctcagagca cgagcacttt acagacttca 421 acgccacgtc ggcactcttc tgggaacagc acgatcttgt gtatggcgac tggactagcg 481 gcgagaactc agacggctgc tacgagcact ttgctgagct cgatatccca cagagcgtcc 541 agcagaacgg ctccatctac atccacgttt acttcaccaa gagtggcttc cacccagacc 601 cccggcagaa ggccctgtac cgccggcttg ccacagtcca catgtcccgg atgatcaaca 661 aatacaagcg cagacgattt cagaaaacca agaacctgct gacaggagag acagaagcgg 721 acccagaaat gatcaagagg gctgaggact atgggcctgt ggaggtgatc tcccattggc 781 accccaacat caccatcaac atcgtggacg accacacgcc gtgggtgaag ggcagtgtgc 841 cccctcccct ggatcaatat gtgaagttcg acgccgtgag cggtgactac tatcccatca 901 tctacttcaa tgactactgg aacctgcaga aggactacta ccccatcaac gagagcctgg 961 ccagcctgcc gctccgcgtc tccttctgcc cgctctcgct ttggcgctgg cagctctatg 1021 ctgcccagag caccaagtcg ccctggaact tcctgggtga tgagttgtac gagcagtcag 1081 atgaggagca ggactcggtg aaggtggccc tgctggagac caacccctac ctgctggcgc 1141 tcaccatcat cgtgtctatc gttcacagtg tcttcgagtt cctggccttc aagaatgata 1201 tccagttctg gaacagccgg cagtccctgg agggcctgtc cgtgcgctcc gtcttcttcg 1261 gcgttttcca gtcattcgtg gtcctcctct acatcctgga caacgagacc aacttcgtgg 1321 tccaggtcag cgtcttcatt ggggtcctca tcgacctctg gaagatcacc aaggtcatgg 1381 acgtccggct ggaccgagag cacagggtgg caggaatctt cccccgccta tccttcaagg 1441 acaagtccac gtatatcgag tcctcgacca aagtgtatga tgatatggca ttccggtacc 1501 tgtcctggat cctcttcccg ctcctgggct gctatgccgt ctacagtctt ctgtacctgg 1561 agcacaaggg ctggtactcc tgggtgctca gcatgctcta cggcttcctg ctgaccttcg 1621 gcttcatcac catgacgccc cagctcttca tcaactacaa gctcaagtct gtggcccacc 1681 ttccctggcg catgctcacc tacaaggccc tcaacacatt catcgacgac ctgttcgcct 1741 ttgtcatcaa gatgcccgtt atgtaccgga tcggctgcct gcgggacgat gtggttttct 1801 tcatctacct cTACCAACGG TGGATCTACC GCGTCGACCC CACCCGAGTC AACGAGTTTG 1861 GCATGAGTGG AGAAGACCCC ACAGCTGCCG CCCCCGTGGC CGAGGTTCCC ACAGCAGCAG 1921 GGGCCCTCAC GCCCACACCT GCACCCACCA CGACCACCGC CACCAGGGAG GAGGCCTCCA 1981 CGTCCCTGCC CACCAAGCCC ACCCAGGGGG CCAGCTCTGC CAGCGAGCCC CAGGGTTTGT 2041 TTGTGGAGGC GCTGTCTGTC CCTCTGTCCC TCTGTGTTTC CAGCCATCTC GCCCTGCCAG 2101 CCCAGCACCA CTGGGAATCA TGGTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 2161 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 2221 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC CAGGTAATGC 2281 TACACACGGG TTTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 2341 att