Construct: ORF TRCN0000475070
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000600.1_s317c1
- Derived from:
- ccsbBroadEn_11898
- DNA Barcode:
- TAAATAGTGGTCGTTCTTAACGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM208 (29100)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475070
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29100 | TMEM208 | transmembrane protein 208 | NM_014187.4 | 57.8% | 57.8% | 301_519del |
| 2 | human | 29100 | TMEM208 | transmembrane protein 208 | NR_134524.2 | 37.4% | 1_89del;390_802del | |
| 3 | human | 29100 | TMEM208 | transmembrane protein 208 | NM_001318217.2 | 17.3% | 17.3% | 0_1ins210;91_309del |
| 4 | mouse | 66320 | Tmem208 | transmembrane protein 208 | XM_006531275.3 | 90% | 96% | (many diffs) |
| 5 | mouse | 66320 | Tmem208 | transmembrane protein 208 | XM_006531276.3 | 90% | 96% | (many diffs) |
| 6 | mouse | 66320 | Tmem208 | transmembrane protein 208 | XM_006531277.3 | 90% | 96% | (many diffs) |
| 7 | mouse | 66320 | Tmem208 | transmembrane protein 208 | NM_025486.2 | 52% | 55.4% | (many diffs) |
| 8 | mouse | 66320 | Tmem208 | transmembrane protein 208 | XM_006531274.3 | 50% | 53% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 369
- ORF length:
- 300
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat GGCGCCCAAG GGCAAAGTGG GCACGAGAGG GAAGAAGCAG ATATTTGAAG 121 AGAACAGAGA GACTCTGAAG TTCTACCTGC GGATCATACT GGGGGCCAAT GCCATTTACT 181 GCCTTGTGAC GTTGGTCTTC TTTTACTCAT CTGCCTCATT TTGGGCCTGG TTGGCCCTGG 241 GCTTTAGTCT GGCAGTGTAT GGGGCCAGCT ACCACTCTAT GAGCTCGATG GCACGAGCAG 301 CGTTCTCTGA GGATGGGGCC CTGATGGATG GTGGCATGGA CCTCAACATG GAGCAGGGCA 361 TGGCAGAGTT GCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 421 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 481 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATAAATA GTGGTCGTTC TTAACGTTAC 541 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt