Construct: ORF TRCN0000475071
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000889.1_s317c1
- Derived from:
- ccsbBroadEn_09620
- DNA Barcode:
- CAGACGAAAGCTCACAATCTTGGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ABHD12B (145447)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475071
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 145447 | ABHD12B | abhydrolase domain containi... | NM_181814.1 | 99.8% | 99.6% | 769T>C |
| 2 | human | 145447 | ABHD12B | abhydrolase domain containi... | NM_181533.3 | 88.7% | 87.7% | (many diffs) |
| 3 | human | 145447 | ABHD12B | abhydrolase domain containi... | NM_001206673.2 | 78.6% | 78.4% | 104_334del;1000T>C |
| 4 | human | 145447 | ABHD12B | abhydrolase domain containi... | XM_011536474.2 | 78.1% | 75.2% | (many diffs) |
| 5 | human | 145447 | ABHD12B | abhydrolase domain containi... | XM_006720052.1 | 65.6% | 64.2% | (many diffs) |
| 6 | human | 145447 | ABHD12B | abhydrolase domain containi... | XM_017021031.1 | 54.6% | 52.2% | (many diffs) |
| 7 | human | 145447 | ABHD12B | abhydrolase domain containi... | XM_011536472.1 | 51.7% | 50.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 924
- ORF length:
- 855
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggacgcgcag gactgccagg cggccgcatc gcccgagccg cccgggcccc 121 cagcccgtag ctgcgtggcc gcctggtggg acatggtcga ccgcaacctg cggcacacag 181 tccccagctg ccggggggaa gatgccaagg ggaaggactg ttgctggtat gaagcagccc 241 ttcgtgatgg gaacccaatt attgtttatc ttcatggcag tgcagaacac agggcagctt 301 cgcacagact gaagctggta aaggtgctga gtgatggtgg ctttcatgTC TTGTCTGTTG 361 ACTACAGAGG ATTTGGGGAC TCTACAGGTA AGCCCACAGA GGAGGGACTG ACTACGGATG 421 CCATTTGTGT CTATGAGTGG ACCAAGGCAA GAAGTGGCAT CACTCCCGTG TGTCTCTGGG 481 GCCACTCTCT GGGTACAGGA GTTGCAACAA ATGCTGCAAA AGTGCTAGAA GAAAAAGGAT 541 GCCCAGTTGA TGCTATTGTC TTGGAAGCTC CATTTACCAA CATGTGGGTT GCAAGTATCA 601 ATTATCCCTT GTTAAAGATT TACCGGAACA TTCCAGGATT TTTACGTACA CTTATGGATG 661 CCCTGAGAAA AGACAAAATA ATCTTTCCTA ATGATGAAAA TGTTAAATTC CTTTCTTCTC 721 CTCTTCTCAT CTTACATGGA GAGGATGACA GGACAGTGCC TTTGGAGTAT GGGAAAAAGC 781 TCTATGAAAT TGCACGCAAT GCATACAGGA ACAAAGAGAG GGTCAAGATG GTTATCCTTC 841 CTCCTGGCTT CCAACACAAC CTGCTTTGTA AAAGCCCCAC ACTGTTAATA ACCGTGAGAG 901 ATTTCCTGAG CAAGCAGTGG TCATTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 961 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1021 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC AGACGAAAGC 1081 TCACAATCTT GGCACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1141 att