Construct: ORF TRCN0000475073
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003977.1_s317c1
- Derived from:
- ccsbBroadEn_03105
- DNA Barcode:
- CTGGTATTAACACCGTTGCAGGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EPN1 (29924)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475073
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29924 | EPN1 | epsin 1 | NM_013333.3 | 100% | 100% | |
| 2 | human | 29924 | EPN1 | epsin 1 | NM_001321263.1 | 95.6% | 95.6% | 602_676del |
| 3 | human | 29924 | EPN1 | epsin 1 | NM_001130072.2 | 95.4% | 95.4% | 602_676del;1175_1177delCAG |
| 4 | human | 29924 | EPN1 | epsin 1 | NM_001130071.1 | 83% | 83% | 1_333del;1433_1435delCAG |
| 5 | mouse | 13854 | Epn1 | epsin 1 | XM_017321973.1 | 87.3% | 94.7% | (many diffs) |
| 6 | mouse | 13854 | Epn1 | epsin 1 | XM_017321974.1 | 87.3% | 94.7% | (many diffs) |
| 7 | mouse | 13854 | Epn1 | epsin 1 | XM_006539532.3 | 87.2% | 94.7% | (many diffs) |
| 8 | mouse | 13854 | Epn1 | epsin 1 | NM_001252454.1 | 83.5% | 90.6% | (many diffs) |
| 9 | mouse | 13854 | Epn1 | epsin 1 | NM_010147.4 | 83.5% | 90.6% | (many diffs) |
| 10 | mouse | 13854 | Epn1 | epsin 1 | XM_006539529.3 | 83.5% | 90.6% | (many diffs) |
| 11 | mouse | 13854 | Epn1 | epsin 1 | XM_006539531.3 | 83.5% | 90.6% | (many diffs) |
| 12 | mouse | 13854 | Epn1 | epsin 1 | XM_006539533.1 | 83.5% | 90.6% | (many diffs) |
| 13 | mouse | 13854 | Epn1 | epsin 1 | XM_006539534.1 | 83.5% | 90.6% | (many diffs) |
| 14 | mouse | 13854 | Epn1 | epsin 1 | XM_006539535.1 | 83.5% | 90.6% | (many diffs) |
| 15 | mouse | 13854 | Epn1 | epsin 1 | XM_006539536.1 | 83.5% | 90.6% | (many diffs) |
| 16 | mouse | 13854 | Epn1 | epsin 1 | XM_006539537.1 | 71.2% | 77.2% | (many diffs) |
| 17 | mouse | 13854 | Epn1 | epsin 1 | XM_006539538.1 | 58.7% | 61.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1716
- ORF length:
- 1650
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc gacctcgtcc ttgaggcgcc agatgaagaa catcgtccac aactactcag 121 aggcggagat caaggttcga gaggccacga gcaatgaccc ctggggccca tccagctccc 181 tcatgtcaga gattgccgac ctcacctaca acgttgtcgc cttctcggag atcatgagca 241 tgatctggaa gcggctcaat gaccatggca agaactggcg tcacgtttac aaggccatga 301 cgctgatgga gtacctcatc aagaccggct cggagcgcgt gtcgcagcag tgcaaggaga 361 acatgtacgc cgtgcagacg ctgaaggact tccagtacgt ggaccgcgac ggcaaggacc 421 agggcgtgaa cgtgcgtgag aaagctaagc agctggtggc cctgctgcgc gacgaggacc 481 ggctgcggga agagcgggcg cacgcgctca agaccaagga aaagctggca cagaccgcca 541 cggcctcatc agcagctgtg ggctcaggcc cccctcccga ggcggagcag gcgtggccgc 601 agagcagcgg ggaggaggag ctgcagctcc agctggccct ggccatgagc aaggaggagg 661 ccgaccagga ggagcggatc cgtcgcgggg atgacctgcg gctgcagatg gcaatcgagg 721 agagcaagag ggagactggg ggcaaggagg agtcgtccct catggacctt gctgacgtct 781 tcacggcccc agctcctgcc ccgaccacag acccctgggg gggcccagca cccatggctg 841 ctgccgtccc cacggctgcc cccacctcgg acccctgggg cggcccccct gtccctccag 901 ctgctgatcc ctggggaggt ccagccccca cgccggcctc tggggacccc tggaggcctg 961 ctgcccctgc aggaccctca gttgaccctt ggggtgggac cccagcccct gcagctgggg 1021 aggggcccac gcctgatcca tggggaagtt ccgatggtgg ggtcccggtc agtgggccct 1081 cagcctccga tccctggaca ccggccccgg ccttctcaga tccctgggga gggtcacctg 1141 ccaagcccag caccaatggc acaacagccg ggggattcga cacggagccc gacgagttct 1201 ctgactttga ccgactccgc acggcactgc cgacctccgg gagcagcgca ggagagctgg 1261 agctgctggc aggagaggtg ccggcccgaa gcccTGGGGC GTTTGACATG AGTGGGGTCA 1321 GGGGATCTCT GGCTGAGGCT GTGGGCAGCC CCCCACCTGC AGCCACACCA ACTCCCACGC 1381 CCCCCACCCG GAAGACGCCG GAGTCATTCC TGGGGCCCAA TGCAGCCCTC GTCGACCTGG 1441 ACTCGCTGGT GAGCCGGCCG GGCCCCACGC CGCCTGGAGC CAAGGCCTCC AACCCCTTCC 1501 TGCCAGGCGG AGGCCCAGCC ACTGGCCCTT CCGTCACCAA CCCCTTCCAG CCCGCGCCTC 1561 CCGCGACGCT CACCCTGAAC CAGCTCCGTC TCAGTCCTGT GCCTCCCGTC CCTGGAGCGC 1621 CACCCACGTA CATCTCTCCC CTTGGCGGGG GCCCTGGCCT GCCCCCCATG ATGCCCCCGG 1681 GCCCCCCGGC CCCCAACACT AATCCCTTCC TCCTATACCC AACTTTCTTG TACAAAGTGG 1741 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1801 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1861 ACTGGTATTA ACACCGTTGC AGGCCACGCG TTAAGTCgac aatcaacctc tggattacaa 1921 aatttgtgaa agatt