Construct: ORF TRCN0000475074

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017399.1_s317c1
Derived from:
ccsbBroadEn_15380
DNA Barcode:
AGAAGCTGTACGCAAACCTCGGCC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CDK7 (1022)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000475074
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1022 CDK7 cyclin dependent kinase 7 NM_001799.4 99.9% 99.7% 389A>G
2 human 1022 CDK7 cyclin dependent kinase 7 NM_001324069.2 87.8% 87.5% 0_1ins123;2_3delTGinsAA;266A>G
3 human 1022 CDK7 cyclin dependent kinase 7 NM_001324070.2 83.4% 83.2% 126_127ins171;218A>G
4 human 1022 CDK7 cyclin dependent kinase 7 NM_001324071.2 83.4% 73.7% 0_1ins34;125_126ins137;218A>G
5 human 1022 CDK7 cyclin dependent kinase 7 NM_001324072.1 73% 72.8% 0_1ins279;110A>G
6 human 1022 CDK7 cyclin dependent kinase 7 NM_001324074.2 73% 72.8% 0_1ins279;110A>G
7 human 1022 CDK7 cyclin dependent kinase 7 NM_001324075.2 73% 72.8% 0_1ins279;110A>G
8 human 1022 CDK7 cyclin dependent kinase 7 NM_001324077.1 73% 72.8% 0_1ins279;110A>G
9 human 1022 CDK7 cyclin dependent kinase 7 NM_001324078.2 73% 72.8% 0_1ins279;110A>G
10 human 1022 CDK7 cyclin dependent kinase 7 XM_011543094.2 66% 65.8% 0_1ins351;38A>G
11 human 1022 CDK7 cyclin dependent kinase 7 NR_136690.2 64.3% (many diffs)
12 mouse 12572 Cdk7 cyclin-dependent kinase 7 NM_009874.3 87.5% 94.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1104
ORF length:
1038
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc tctggacgtg aagtctcggg caaagcgtta tgagaagctg gacttccttg
121gggagggaca gtttgccacc gtttacaagg ccagagataa gaacaccaac caaattgtcg
181ccattaagaa aatcaaactt ggacatagat cagaagctaa agatggtata aatagaaccg
241ccttaagaga gataaaatta ttacaggagc taagtcatcc aaatataatt ggtctccttg
301atgcttttgg acataaatct aatattagcc ttgtctttga ttttatggaa actgatctag
361aggttataat aaaggataat agtcttgtgc tgacaccatc acacatcaaa gcctacatgt
421tgatgactct tcaaggatta gaatatttac atcgacattg gatcctacat agggatctga
481aaccaaacaa cttgttgcta gatgaaaatg gagttctaaa actggcagat tttggcctgg
541ccaaatcttt tgGGAGCCCC AATAGAGCTT ATACACATCA GGTTGTAACC AGGTGGTATC
601GGGCCCCCGA GTTACTATTT GGAGCTAGGA TGTATGGTGT AGGTGTGGAC ATGTGGGCTG
661TTGGCTGTAT ATTAGCAGAG TTACTTCTAA GGGTTCCTTT TTTGCCAGGA GATTCAGACC
721TTGATCAGCT AACAAGAATA TTTGAAACTT TGGGCACACC AACTGAGGAA CAGTGGCCGG
781ACATGTGTAG TCTTCCAGAT TATGTGACAT TTAAGAGTTT CCCTGGAATA CCTTTGCATC
841ACATCTTCAG TGCAGCAGGA GACGACTTAC TAGATCTCAT ACAAGGCTTA TTCTTATTTA
901ATCCATGTGC TCGAATTACG GCCACACAGG CACTGAAAAT GAAGTATTTC AGTAATCGGC
961CAGGGCCAAC ACCTGGATGT CAGCTGCCAA GACCAAACTG TCCAGTGGAA ACCTTAAAGG
1021AGCAATCAAA TCCAGCTTTG GCAATAAAAA GGAAAAGAAC AGAGGCCTTA GAACAAGGAG
1081GATTGCCCAA GAAACTAATT TTTTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA
1141AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC
1201TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA GAAGCTGTAC
1261GCAAACCTCG GCCACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag
1321att

Download FASTA (ORF) (Full)