Construct: ORF TRCN0000475086
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008731.1_s317c1
- Derived from:
- ccsbBroadEn_13852
- DNA Barcode:
- TGACCTAACGTTTGTTCGTAATGG
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ELF3 (1999)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475086
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1999 | ELF3 | E74 like ETS transcription ... | NM_001114309.2 | 99.9% | 99.7% | 1109delG |
| 2 | human | 1999 | ELF3 | E74 like ETS transcription ... | NM_004433.5 | 99.9% | 99.7% | 1109delG |
| 3 | human | 1999 | ELF3 | E74 like ETS transcription ... | XM_005244942.3 | 57.1% | 54.6% | (many diffs) |
| 4 | mouse | 13710 | Elf3 | E74-like factor 3 | NM_007921.3 | 84.4% | 87% | (many diffs) |
| 5 | mouse | 13710 | Elf3 | E74-like factor 3 | NM_001163131.1 | 80.1% | 82.6% | (many diffs) |
| 6 | mouse | 13710 | Elf3 | E74-like factor 3 | XM_006529125.1 | 70.4% | 72.6% | (many diffs) |
| 7 | mouse | 13710 | Elf3 | E74-like factor 3 | XM_006529124.1 | 67.4% | 69.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1179
- ORF length:
- 1113
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgcaacctgt gagattagca acatttttag caactacttc agtgcgatgt 121 acagctcgga ggactccacc ctggcctctg ttccccctgc tgccaccttt ggggccgatg 181 acttggtact gaccctgagc aacccccaga tgtcattgga gggtacagag aaggccagct 241 ggttggggga acagccccag ttctggtcga agacgcaggt tctggactgg atcagctacc 301 aagtggagaa gaacaagtac gacgcaagcg ccattgactt ctcacgatgt gacatggatg 361 gcgccaccct ctgcaattgt gcccttgagg agctgcgtct ggtctttggg cctctggggg 421 accaactcca tgcccagctg cgagacctca cttccagctc ttctgatgag ctcagttgga 481 tcattgagct gctggagaag gatggcatgg ccttccagga ggccctagac ccagggccct 541 ttgaccaggg cagccccttt gcccaggagc tgctggacga cggtcagcaa gccagcccct 601 accaccccgg cagctgtggc gcaggagccc cctcccctgg cagctctgac gtctccaccg 661 cagggactgg tgcttctcgg agctcccact cctcagactc cggtggaagt gacgtggacc 721 tggatcccac tgatggcaag ctcttcccca gcgatggttt tcgtgactgc aagaaggggg 781 atcccaagca cgggaagcgg aaacgaggcc ggccccgaaa gctgagcaaa gagtactggg 841 actgtctcga gggcaagaag agcaagcacg cgcccagagg cacccacctg tgggagttca 901 tccgggacat cctcatccac ccggagctca acgagggcct catgaagtgg gagaatcggc 961 atgaaggcgt cttcaagttc ctgcgctccg aggctgtggc ccaactatgg ggCCAAAAGA 1021 AAAAGAACAG CAACATGACC TACGAGAAGC TGAGCCGGGC CATGAGGTAC TACTACAAAC 1081 GGGAGATCCT GGAACGGGTG GATGGCCGGC GACTCGTCTA CAAGTTTGGC AAAAACTCAA 1141 GCGGCTGGAA GGAGGAAGAG GTTCTCCAGA GTCGAACTGC CCAACTTTCT TGTACAAAGT 1201 GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG 1261 AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA 1321 CGATGACCTA ACGTTTGTTC GTAATGGACG CGTTAAGTCg acaatcaacc tctggattac 1381 aaaatttgtg aaagatt