Construct: ORF TRCN0000475108
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008175.3_s317c1
- Derived from:
- ccsbBroadEn_12685
- DNA Barcode:
- ATACCTCAAAACCATGTCACAAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ALPK1 (80216)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475108
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80216 | ALPK1 | alpha kinase 1 | NM_001102406.2 | 87.2% | 83.9% | 3124delA;3257_3732del |
| 2 | human | 80216 | ALPK1 | alpha kinase 1 | NM_025144.4 | 87.2% | 83.9% | 3124delA;3257_3732del |
| 3 | human | 80216 | ALPK1 | alpha kinase 1 | NM_001253884.1 | 80.9% | 76.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3321
- ORF length:
- 3255
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa taatcaaaaa gtggtagctg tgctactgca agagtgcaag caagtgctgg 121 atcagctctt gttggaagcg ccagatgtgt cggaagagga caagagcgag gaccagcgct 181 gcagagcttt actccccagc gagttaagga ccctgatcca ggaggcaaag gaaatgaagt 241 ggcccttcgt gcctgaaaag tggcagtaca aacaagccgt gggcccagag gacaaaacaa 301 acctgaagga tgtgattggc gccgggttgc agcagttact ggcgtccctg agggcctcca 361 tcctcgctcg ggactgtgcg gctgcggcgg ctattgtgtt cttggtggac cggttcctgt 421 atgggctcga cgtctctgga aaacttctgc aggtcgccaa aggtctccac aagttgcagc 481 cagccacgcc aattgccccg caggtggtta ttcgccaagc ccgaatctcc gtgaactcag 541 gaaaactttt aaaagcagag tatattctga gcagtctaat aagcaacaat ggagcaacgg 601 gtacctggct gtacagaaat gaaagtgaca aggtcctggt gcagtcggtc tgtatacaga 661 tcagagggca gattctgcaa aagctgggga tgtggtacga agcagcagag ttaatatggg 721 cctccattgt aggatatttg gcacttcctc agccggataa aaagggcctc tccacgtcgc 781 taggtatact ggcagacatc tttgtttcca tgagcaagaa cgattatgaa aagtttaaaa 841 acaatccaca aattaatttg agcctgctga aggagtttga ccaccatttg ctgtccgctg 901 cagaagcctg caagctggca gctgccttca gtgcctatac gccgctcttc gtgctcacag 961 ctgtgaatat ccgtggcacg tgtttattgt cctacagtag ttcaaatgac tgtcctccag 1021 aattgaaaaa cttacatctg tgtgaagcca aagaggcctt tgagattggc ctcctcacca 1081 agagagatga tgagcctgtt actggaaaac aggagcttca cagctttgtc aaagctgctt 1141 tcggtctcac cacagtgcac agaaggctcc atggggagac agggacggtc catgcagcaa 1201 gtcagctctg taaggaagca atggggaagc tgtacaattt cagcacttcc tccagaagtc 1261 aggacagaga agctctgtct caagaagtta tgtctgtgat tgcccaggtg aaggaacatt 1321 tacaagttca aagcttctca aatgtagatg acagatctta tgttcccgag agtttcgagt 1381 gcaggttgga taaacttatc ttgcatgggc aaggggattt ccaaaaaatc cttgacacct 1441 attcacagca ccatacttcg gtgtgtgaag tatttgaaag tgattgtgga aacaacaaaa 1501 atgaacagaa agatgcaaaa acaggagtct gcatcactgc tctaaaaaca gaaataaaaa 1561 acatagatac tgtgagtact actcaagaaa agccacattg tcaaagagac acaggaatat 1621 cttcctccct aatgggtaag aatgttcaga gggaactcag aaggggagga aggagaaact 1681 ggacccattc tgatgcattt cgagtctcct tggatcaaga tgtggagact gagactgagc 1741 catcggacta cagcaatggt gagggagctg ttttcaacaa gtctctgagt ggcagccaga 1801 cttccagtgc ttggagcaac ttatcagggt ttagttcctc tgcaagctgg gaggaagtga 1861 attatcacgt tgacgacagg tcagccagaa aagagcctgg caaagaacat ctggtggaca 1921 ctcagtgttc cactgccttg tctgaggagc tagagaatga cagggaaggc agagctatgc 1981 attcattgca ttcacagctt catgatctct ctcttcagga acccaacaat gacaatttgg 2041 agccttctca aaatcagcca cagcaacaga tgcccttgac acccttctcg cctcataata 2101 ccccaggcat tttcttggcc cctggtgcag ggcttctaga aggagctcca gaaggtatcc 2161 aggaagtcag aaatatggga cccagaaata cttctgctca ctccagaccc tcatatcgtt 2221 ctgcttcttg gtcttctgat tctggtaggc ccaagaatat gggcacacat ccttcagtcc 2281 aaaaagaaga agcctttgaa ataattgttg agtttccaga aaccaactgc gatgtcaaag 2341 acaggcaggg gaaagagcag ggagaagaaa ttagtgaaag aggcgcaggc cctacattta 2401 aagctagtcc ctcctgggtt gacccagaag gagaaacagc agaaagcact gaagatgcac 2461 ccttagactt tcacagggtc ctgcacaatt ctctgggaaa catttccatg ctgccatgta 2521 gctccttcac ccctaattgg cctgttcaaa atcctgactc cagaaaaagt ggtggcccag 2581 tcgcagagca gggcatcgac cctgatgcct ccacagtgga tgaggagggg caactgctcg 2641 acagcatgga tgttccctgc acaaatgggc acggctctca tagactgtgc attctgagac 2701 agccgcctgg tcagagggcg gagaccccca attcctctgt aagcggtaac atcctcttcc 2761 ctgtcctcag cgaggactgc actaccacag aggaaggaaa tcagcctgga aacatgctaa 2821 actgcagcca gaactccagc tcatcctcag tgtggtggct gaaatcacct gcattttcca 2881 gtggttcttc TGAGGGGGAC AGCCCTTGGT CCTATCTGAA TTCCAGTGGG AGTTCTTGGG 2941 TTTCATTGCC GGGAAAGATG AGGAAAGAGA TCCTTGAGGC TCGCACCTTG CAACCTGATG 3001 ACTTTGAAAA GCTGTTGGCA GGAGTGAGGC ATGATTGGCT GTTTCAGAGA CTAGAGAATA 3061 CGGGGGTTTT TAAGCCCAGT CAACTCCACC GAGCACATAG TGCTCTTTTG TTAAAATATT 3121 CAAAAAAATC TGAACTGTGG ACGGCCCAGG AAACTATTGT CTATTTGGGG GACTACTTGA 3181 CTGTGAAGAA AAAGGCAGAC AAAGAAATGC TTTTTGGGTT CATCATCTTC ATCAAGAAGA 3241 AATTCTGGGG AGGTATGTTG GGAAAGACTA TAAGGAGCAG AAGGGGCTCT GGCACCACTT 3301 CACTGATGTG GAGCGACAGA TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 3361 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 3421 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAATAC CTCAAAACCA 3481 TGTCACAAAA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt