Construct: ORF TRCN0000475108

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008175.3_s317c1
Derived from:
ccsbBroadEn_12685
DNA Barcode:
ATACCTCAAAACCATGTCACAAAA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ALPK1 (80216)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000475108
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 80216 ALPK1 alpha kinase 1 NM_001102406.2 87.2% 83.9% 3124delA;3257_3732del
2 human 80216 ALPK1 alpha kinase 1 NM_025144.4 87.2% 83.9% 3124delA;3257_3732del
3 human 80216 ALPK1 alpha kinase 1 NM_001253884.1 80.9% 76.6% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
3321
ORF length:
3255
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgaa taatcaaaaa gtggtagctg tgctactgca agagtgcaag caagtgctgg
121atcagctctt gttggaagcg ccagatgtgt cggaagagga caagagcgag gaccagcgct
181gcagagcttt actccccagc gagttaagga ccctgatcca ggaggcaaag gaaatgaagt
241ggcccttcgt gcctgaaaag tggcagtaca aacaagccgt gggcccagag gacaaaacaa
301acctgaagga tgtgattggc gccgggttgc agcagttact ggcgtccctg agggcctcca
361tcctcgctcg ggactgtgcg gctgcggcgg ctattgtgtt cttggtggac cggttcctgt
421atgggctcga cgtctctgga aaacttctgc aggtcgccaa aggtctccac aagttgcagc
481cagccacgcc aattgccccg caggtggtta ttcgccaagc ccgaatctcc gtgaactcag
541gaaaactttt aaaagcagag tatattctga gcagtctaat aagcaacaat ggagcaacgg
601gtacctggct gtacagaaat gaaagtgaca aggtcctggt gcagtcggtc tgtatacaga
661tcagagggca gattctgcaa aagctgggga tgtggtacga agcagcagag ttaatatggg
721cctccattgt aggatatttg gcacttcctc agccggataa aaagggcctc tccacgtcgc
781taggtatact ggcagacatc tttgtttcca tgagcaagaa cgattatgaa aagtttaaaa
841acaatccaca aattaatttg agcctgctga aggagtttga ccaccatttg ctgtccgctg
901cagaagcctg caagctggca gctgccttca gtgcctatac gccgctcttc gtgctcacag
961ctgtgaatat ccgtggcacg tgtttattgt cctacagtag ttcaaatgac tgtcctccag
1021aattgaaaaa cttacatctg tgtgaagcca aagaggcctt tgagattggc ctcctcacca
1081agagagatga tgagcctgtt actggaaaac aggagcttca cagctttgtc aaagctgctt
1141tcggtctcac cacagtgcac agaaggctcc atggggagac agggacggtc catgcagcaa
1201gtcagctctg taaggaagca atggggaagc tgtacaattt cagcacttcc tccagaagtc
1261aggacagaga agctctgtct caagaagtta tgtctgtgat tgcccaggtg aaggaacatt
1321tacaagttca aagcttctca aatgtagatg acagatctta tgttcccgag agtttcgagt
1381gcaggttgga taaacttatc ttgcatgggc aaggggattt ccaaaaaatc cttgacacct
1441attcacagca ccatacttcg gtgtgtgaag tatttgaaag tgattgtgga aacaacaaaa
1501atgaacagaa agatgcaaaa acaggagtct gcatcactgc tctaaaaaca gaaataaaaa
1561acatagatac tgtgagtact actcaagaaa agccacattg tcaaagagac acaggaatat
1621cttcctccct aatgggtaag aatgttcaga gggaactcag aaggggagga aggagaaact
1681ggacccattc tgatgcattt cgagtctcct tggatcaaga tgtggagact gagactgagc
1741catcggacta cagcaatggt gagggagctg ttttcaacaa gtctctgagt ggcagccaga
1801cttccagtgc ttggagcaac ttatcagggt ttagttcctc tgcaagctgg gaggaagtga
1861attatcacgt tgacgacagg tcagccagaa aagagcctgg caaagaacat ctggtggaca
1921ctcagtgttc cactgccttg tctgaggagc tagagaatga cagggaaggc agagctatgc
1981attcattgca ttcacagctt catgatctct ctcttcagga acccaacaat gacaatttgg
2041agccttctca aaatcagcca cagcaacaga tgcccttgac acccttctcg cctcataata
2101ccccaggcat tttcttggcc cctggtgcag ggcttctaga aggagctcca gaaggtatcc
2161aggaagtcag aaatatggga cccagaaata cttctgctca ctccagaccc tcatatcgtt
2221ctgcttcttg gtcttctgat tctggtaggc ccaagaatat gggcacacat ccttcagtcc
2281aaaaagaaga agcctttgaa ataattgttg agtttccaga aaccaactgc gatgtcaaag
2341acaggcaggg gaaagagcag ggagaagaaa ttagtgaaag aggcgcaggc cctacattta
2401aagctagtcc ctcctgggtt gacccagaag gagaaacagc agaaagcact gaagatgcac
2461ccttagactt tcacagggtc ctgcacaatt ctctgggaaa catttccatg ctgccatgta
2521gctccttcac ccctaattgg cctgttcaaa atcctgactc cagaaaaagt ggtggcccag
2581tcgcagagca gggcatcgac cctgatgcct ccacagtgga tgaggagggg caactgctcg
2641acagcatgga tgttccctgc acaaatgggc acggctctca tagactgtgc attctgagac
2701agccgcctgg tcagagggcg gagaccccca attcctctgt aagcggtaac atcctcttcc
2761ctgtcctcag cgaggactgc actaccacag aggaaggaaa tcagcctgga aacatgctaa
2821actgcagcca gaactccagc tcatcctcag tgtggtggct gaaatcacct gcattttcca
2881gtggttcttc TGAGGGGGAC AGCCCTTGGT CCTATCTGAA TTCCAGTGGG AGTTCTTGGG
2941TTTCATTGCC GGGAAAGATG AGGAAAGAGA TCCTTGAGGC TCGCACCTTG CAACCTGATG
3001ACTTTGAAAA GCTGTTGGCA GGAGTGAGGC ATGATTGGCT GTTTCAGAGA CTAGAGAATA
3061CGGGGGTTTT TAAGCCCAGT CAACTCCACC GAGCACATAG TGCTCTTTTG TTAAAATATT
3121CAAAAAAATC TGAACTGTGG ACGGCCCAGG AAACTATTGT CTATTTGGGG GACTACTTGA
3181CTGTGAAGAA AAAGGCAGAC AAAGAAATGC TTTTTGGGTT CATCATCTTC ATCAAGAAGA
3241AATTCTGGGG AGGTATGTTG GGAAAGACTA TAAGGAGCAG AAGGGGCTCT GGCACCACTT
3301CACTGATGTG GAGCGACAGA TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC
3361CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG
3421AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAATAC CTCAAAACCA
3481TGTCACAAAA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)