Construct: ORF TRCN0000475123
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013699.1_s317c1
- Derived from:
- ccsbBroadEn_08407
- DNA Barcode:
- AGCGCATTTCAGTTTACTGAGTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FBLIM1 (54751)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475123
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54751 | FBLIM1 | filamin binding LIM protein 1 | NM_001350151.2 | 99.9% | 99.7% | 572C>T |
| 2 | human | 54751 | FBLIM1 | filamin binding LIM protein 1 | NM_017556.4 | 99.9% | 99.7% | 572C>T |
| 3 | human | 54751 | FBLIM1 | filamin binding LIM protein 1 | XM_011541617.2 | 99.9% | 99.7% | 572C>T |
| 4 | human | 54751 | FBLIM1 | filamin binding LIM protein 1 | XM_017001525.1 | 99.9% | 99.7% | 572C>T |
| 5 | human | 54751 | FBLIM1 | filamin binding LIM protein 1 | XM_011541618.3 | 84% | 78.8% | 572C>T;888_889ins118;942_943ins59 |
| 6 | human | 54751 | FBLIM1 | filamin binding LIM protein 1 | NM_001024215.1 | 81.7% | 76.6% | (many diffs) |
| 7 | human | 54751 | FBLIM1 | filamin binding LIM protein 1 | XM_005245900.1 | 81.7% | 76.6% | (many diffs) |
| 8 | human | 54751 | FBLIM1 | filamin binding LIM protein 1 | XM_005245901.1 | 81.7% | 76.6% | (many diffs) |
| 9 | human | 54751 | FBLIM1 | filamin binding LIM protein 1 | XM_005245902.1 | 81.7% | 76.6% | (many diffs) |
| 10 | human | 54751 | FBLIM1 | filamin binding LIM protein 1 | XM_005245903.1 | 81.7% | 76.6% | (many diffs) |
| 11 | human | 54751 | FBLIM1 | filamin binding LIM protein 1 | XM_006710704.3 | 81.7% | 76.6% | (many diffs) |
| 12 | human | 54751 | FBLIM1 | filamin binding LIM protein 1 | XM_006710705.1 | 81.7% | 76.6% | (many diffs) |
| 13 | human | 54751 | FBLIM1 | filamin binding LIM protein 1 | XM_011541616.2 | 81.7% | 76.6% | (many diffs) |
| 14 | human | 54751 | FBLIM1 | filamin binding LIM protein 1 | XM_017001519.2 | 81.7% | 76.6% | (many diffs) |
| 15 | human | 54751 | FBLIM1 | filamin binding LIM protein 1 | XM_017001520.2 | 81.7% | 76.6% | (many diffs) |
| 16 | human | 54751 | FBLIM1 | filamin binding LIM protein 1 | XM_017001521.2 | 81.7% | 76.6% | (many diffs) |
| 17 | human | 54751 | FBLIM1 | filamin binding LIM protein 1 | XM_017001523.1 | 81.7% | 76.6% | (many diffs) |
| 18 | human | 54751 | FBLIM1 | filamin binding LIM protein 1 | XM_017001524.1 | 81.7% | 76.6% | (many diffs) |
| 19 | human | 54751 | FBLIM1 | filamin binding LIM protein 1 | XM_017001526.2 | 81.1% | 79.4% | (many diffs) |
| 20 | human | 54751 | FBLIM1 | filamin binding LIM protein 1 | XM_024447751.1 | 80.2% | 78.6% | (many diffs) |
| 21 | human | 54751 | FBLIM1 | filamin binding LIM protein 1 | NM_001024216.3 | 73.9% | 73.7% | 248_249ins291;281C>T |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1185
- ORF length:
- 1119
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ctcaaagcct gagaagaggg tggcatcgtc tgtctttatc accctggcac 121 ccccgcgccg cgatgtggcc gtggcggagg aagtgaggca ggcagtttgt gaggcccggc 181 gtggccgccc ctgggaggct cctgccccca tgaagacacc cgaggctggc ttggcgggga 241 ggcccagccc ctggacaacc cctggcagag ctgcagccac agtgccggct gcacctatgc 301 agctcttcaa tggaggatgc ccaccccctc ctcctgtcct ggatggtgag gacgtgcttc 361 ctgacctgga cctcctccca ccccctccac cgccccctcc agtgcttctg ccttctgaag 421 aggaggctcc tgctccaatg ggggcctcac tcattgcaga cttagagcag ctgcacctgt 481 ccccgccccc gcccccacca caggccccag cggagggacc ttcagtccag cccggtcccc 541 tcaggcccat ggaggaagag ctgccacctc ccccggcaga acctgttgag aaaggggcat 601 ccacagacat ctgtgccttc tgccacaaga ccgtgttccc ccgagagctg gctgtggagg 661 ccatgaagag gcagtaccat gcccagtgct tcacgtgccg cacctgccgc cgccagctgg 721 ctgggCAGAG CTTCTACCAG AAGGATGGGC GACCCCTCTG CGAACCCTGC TACCAGGACA 781 CACTGGAGAG GTGCGGCAAG TGTGGCGAGG TGGTCCGGGA CCACATCATC AGGGCCCTGG 841 GCCAGGCCTT CCACCCCTCC TGCTTCACGT GTGTGACCTG CGCCCGGTGC ATTGGGGATG 901 AGAGCTTTGC CCTGGGCAGC CAGAACGAGG TGTACTGCCT GGACGACTTC TACAGGAAAT 961 TCGCCCCCGT CTGCAGCATC TGTGAAAATC CCATCATCCC TCGGGATGGG AAAGATGCCT 1021 TCAAAATCGA ATGCATGGGA AGAAACTTCC ATGAAAATTG CTACAGGTGT GAGGACTGCA 1081 GGATCCTCCT GTCTGTCGAG CCCACGGACC AAGGCTGCTA CCCCCTGAAC AACCATCTCT 1141 TCTGCAAGCC ATGCCATGTG AAGCGGAGTG CTGCGGGGTG CTGCTGCCCA ACTTTCTTGT 1201 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1261 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTCGATTTCT TGGCTTTATA TATCTTGTGG 1321 AAAGGACGAA GCGCATTTCA GTTTACTGAG TACACGCGTT AAGTCgacaa tcaacctctg 1381 gattacaaaa tttgtgaaag att