Construct: ORF TRCN0000475127
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013913.1_s317c1
- Derived from:
- ccsbBroadEn_12417
- DNA Barcode:
- TACCTGGATAGTCCGAACTCCGGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EPS15L1 (58513)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475127
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 58513 | EPS15L1 | epidermal growth factor rec... | NM_001258375.1 | 100% | 100% | |
| 2 | human | 58513 | EPS15L1 | epidermal growth factor rec... | XM_017027092.2 | 99.4% | 99.3% | 2249A>T;2250_2251insAAAGTACACCTG |
| 3 | human | 58513 | EPS15L1 | epidermal growth factor rec... | XM_017027091.2 | 94.6% | 94% | (many diffs) |
| 4 | human | 58513 | EPS15L1 | epidermal growth factor rec... | XM_017027090.2 | 94.3% | 93.9% | (many diffs) |
| 5 | human | 58513 | EPS15L1 | epidermal growth factor rec... | XM_017027089.2 | 89.1% | 88.6% | (many diffs) |
| 6 | human | 58513 | EPS15L1 | epidermal growth factor rec... | NM_021235.2 | 87.1% | 86.6% | (many diffs) |
| 7 | human | 58513 | EPS15L1 | epidermal growth factor rec... | XM_017027088.2 | 86.1% | 85.6% | (many diffs) |
| 8 | human | 58513 | EPS15L1 | epidermal growth factor rec... | NM_001258374.3 | 82.7% | 82.3% | (many diffs) |
| 9 | human | 58513 | EPS15L1 | epidermal growth factor rec... | XM_017027087.2 | 82.3% | 81.8% | (many diffs) |
| 10 | human | 58513 | EPS15L1 | epidermal growth factor rec... | XM_017027086.2 | 81.3% | 80.8% | (many diffs) |
| 11 | human | 58513 | EPS15L1 | epidermal growth factor rec... | NM_001258376.1 | 79.5% | 78.8% | (many diffs) |
| 12 | human | 58513 | EPS15L1 | epidermal growth factor rec... | NR_047666.1 | 78.3% | (many diffs) | |
| 13 | human | 58513 | EPS15L1 | epidermal growth factor rec... | NR_047665.1 | 72% | 1_67del;2330_3139del | |
| 14 | mouse | 13859 | Eps15l1 | epidermal growth factor rec... | XM_006509547.3 | 81.4% | 87% | (many diffs) |
| 15 | mouse | 13859 | Eps15l1 | epidermal growth factor rec... | XM_006509548.3 | 81.2% | 87% | (many diffs) |
| 16 | mouse | 13859 | Eps15l1 | epidermal growth factor rec... | XM_017312560.1 | 78.3% | 84.4% | (many diffs) |
| 17 | mouse | 13859 | Eps15l1 | epidermal growth factor rec... | NM_001289859.1 | 78.1% | 84.4% | (many diffs) |
| 18 | mouse | 13859 | Eps15l1 | epidermal growth factor rec... | XM_017312559.1 | 75% | 80.2% | (many diffs) |
| 19 | mouse | 13859 | Eps15l1 | epidermal growth factor rec... | NM_007944.3 | 71.3% | 76.2% | (many diffs) |
| 20 | mouse | 13859 | Eps15l1 | epidermal growth factor rec... | XM_006509544.3 | 70.1% | 74.9% | (many diffs) |
| 21 | mouse | 13859 | Eps15l1 | epidermal growth factor rec... | NM_001122832.1 | 68.6% | 73.1% | (many diffs) |
| 22 | mouse | 13859 | Eps15l1 | epidermal growth factor rec... | XM_006509546.3 | 68.5% | 73.9% | (many diffs) |
| 23 | mouse | 13859 | Eps15l1 | epidermal growth factor rec... | XM_006509545.3 | 67.3% | 72.6% | (many diffs) |
| 24 | mouse | 13859 | Eps15l1 | epidermal growth factor rec... | XR_379032.3 | 67% | (many diffs) | |
| 25 | mouse | 13859 | Eps15l1 | epidermal growth factor rec... | XR_379029.3 | 65.4% | (many diffs) | |
| 26 | mouse | 13859 | Eps15l1 | epidermal growth factor rec... | XR_379030.3 | 63% | (many diffs) | |
| 27 | mouse | 13859 | Eps15l1 | epidermal growth factor rec... | XR_001778398.1 | 62.7% | (many diffs) | |
| 28 | mouse | 13859 | Eps15l1 | epidermal growth factor rec... | XR_379031.3 | 60.1% | (many diffs) | |
| 29 | mouse | 13859 | Eps15l1 | epidermal growth factor rec... | XM_006509550.2 | 41.6% | 41.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2331
- ORF length:
- 2262
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcggcgccg ctcatccccc tctcccagca gattcccact ggaaattcgt 121 tgtatgaatc ttattacaag caggtcgatc cggcatacac agggagggtg ggggcgagtg 181 aagctgcgct ttttctaaag aagtctggcc tctcggacat tatccttggg aagatatggg 241 acttggccga tccagaaggt aaagggttct tggacaaaca gggtttctat gttgcactga 301 gactggtggc ctgtgcacag agtggccatg aagttacctt gagcaatctg aatttgagca 361 tgccaccgcc taaatttcac gacaccagca gccctctgat ggtcacaccg ccctctgcag 421 aggcccactg ggctgtgagg gtggaagaaa aggccaaatt tgatgggatt tttgaaagcc 481 tcttgcccat caatggtttg ctctctggag acaaagtcaa gccagtcctc atgaactcaa 541 agctgcctct tgatgtcctg ggcagggtct gggacctcag tgacattgac aaggatgggc 601 acttggatcg agatgagttc gctgtggcca tgcacttggt gtaccgagcc ctggagaagg 661 agcccgtgcc ctccgccctg cccccgtccc tcatcccacc ctccaagaga aagaagactg 721 tgttccctgg cgccgtcccc gtcctgcctg ccagcccccc accaaaagac agcctccgct 781 ccacgccgtc ccacggcagc gtcagcagcc tcaacagcac agggagcctg tcccccaagc 841 acagcctcaa gcaaacacag ccaacagtga actgggtggt gcccgtggca gacaagatgc 901 gatttgatga gatattcctg aagaccgacc tggacctgga tggctacgtg agtggccagg 961 aggtgaagga gatcttcatg cactcgggcc tcacccagaa ccttctagca cacatatggg 1021 ccctggccga tacgaggcaa acggggaagt taagcaaaga ccaattcgcg ttagctatgt 1081 atttcattca gcagaaggtc agtaaaggca tcgaccctcc tcaagtcctc tcgccggaca 1141 tggtcccgcc ttcggagaga ggcacgcccg gcccggacag ttcaggctct ctcggctccg 1201 gggagtttac tggcgtgaag gagcttgatg acatcagtca agagattgcc cagttacaaa 1261 gagagaaata ttcactggaa caagacattc gagaaaagga agaggcaatc agacagaaaa 1321 ccagcgaggt gcaggaatta caaaatgacc tagaccggga aacaagcagt ttgcaggagc 1381 tcgaggctca gaaacaggat gctcaagacc gcctggacga gatggaccag cagaaggcca 1441 agctccgaga catgctgagc gacgtccggc agaagtgcca ggatgagact cagatgatct 1501 catcactgaa aacgcaaatc caatctcagg aatctgactt aaagtcccag gaagacgatc 1561 tgaaccgagc caagtcggag ctgaaccgat tgcagcagga ggaaacccag ctggagcaga 1621 gcattcaggc tgggcgagtc cagctggaaa ccatcatcaa gtccctgaag tcaacgcaag 1681 acgaaatcaa ccaggcaagg agcaaacttt cccagctgca tgaaagccgc caggaggccc 1741 acaggagcct ggagcagtat gaccaggtgc tcgatggagc ccatggtgcc agcctgaccg 1801 acctggccaa cctgagcgaa ggcgtctccc tggcagagag gggcagtttt ggagccatgg 1861 atgatccttt caaaaataaa gccttgttat ttagcaacaa cacgcaagag ttgcatccgg 1921 atcctttcca gacagaagac cccttcaaaT CTGACCCATT TAAAGGAGCT GACCCCTTCA 1981 AAGGCGACCC GTTCCAGAAT GACCCCTTTG CAGAACAGCA GACAACTTCA ACAGATCCAT 2041 TTGGAGGGGA CCCTTTCAAA GAAAGTGACC CATTCCGTGG CTCTGCCACT GACGACTTCT 2101 TCAAGAAACA GACAAAGAAT GACCCATTTA CCTCGGATCC ATTCACGAAA AACCCTTCCT 2161 TACCTTCGAA GCTCGACCCC TTTGAATCCA GTGATCCCTT TTCATCCTCC AGTGTCTCCT 2221 CAAAAGGATC AGATCCCTTT GGAACCTTAG ATCCCTTCGG AAGTGGGTCC TTCAATAGTG 2281 CTGAAGGCTT TGCCGACTTC AGCCAGATGT CCAAGGTAAA AGTACACCTG TTGCCAACTT 2341 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 2401 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 2461 CTTGTGGAAA GGACGATACC TGGATAGTCC GAACTCCGGC ACGCGTTAAG TCgacaatca 2521 acctctggat tacaaaattt gtgaaagatt