Construct: ORF TRCN0000475138

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015708.1_s317c1
Derived from:
ccsbBroadEn_15676
DNA Barcode:
AACGATTTTACCAGCGTGTTCTGC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
LAPTM4A (9741)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000475138
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9741 LAPTM4A lysosomal protein transmemb... NM_014713.5 100% 100%
2 mouse 17775 Laptm4a lysosomal-associated protei... NM_008640.2 69.6% 73.7% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
765
ORF length:
699
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggt gtccatgagt ttcaagcgga accgcagtga ccggttctac agcacccggt
121gctgcggctg ttgccatgtc cgcaccggga cgatcatcct ggggacctgg tacatggtag
181taaacctatt gatggcaatt ttgctgactg tggaagtgac tcatccaaac tccatgccag
241ctgtcaacat tcagtatgaa gtcatcggta attactattc gtctgagaga atggctgata
301atgcctgtgt tctttttgcc gtctctgttc ttatgtttat aatcagttca atgctggttt
361atggagcaat ttcttatcaa gtgggttggc tgattccatT CTTCTGTTAC CGACTTTTTG
421ACTTCGTCCT CAGTTGCCTG GTTGCTATTA GTTCTCTCAC CTATTTGCCA AGAATCAAAG
481AATATCTGGA TCAACTACCT GATTTTCCCT ACAAAGATGA CCTCCTGGCC TTGGACTCCA
541GCTGCCTCCT GTTCATTGTT CTTGTGTTCT TTGCCTTATT CATCATTTTT AAGGCTTATC
601TAATTAACTG TGTTTGGAAC TGCTATAAAT ACATCAACAA CCGAAACGTG CCGGAGATTG
661CTGTGTACCC TGCCTTTGAA GCACCTCCTC AGTACGTTTT GCCAACCTAT GAAATGGCCG
721TGAAAATGCC TGAAAAAGAA CCACCACCTC CTTACTTACC TGCCTGCCCA ACTTTCTTGT
781ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT
841AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG
901GAAAGGACGA AACGATTTTA CCAGCGTGTT CTGCACGCGT TAAGTCgaca atcaacctct
961ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)