Transcript: Human NM_014713.5

Homo sapiens lysosomal protein transmembrane 4 alpha (LAPTM4A), mRNA.

Source:
NCBI, updated 2019-09-27
Taxon:
Homo sapiens (human)
Gene:
LAPTM4A (9741)
Length:
1365
CDS:
109..810

Additional Resources:

NCBI RefSeq record:
NM_014713.5
NBCI Gene record:
LAPTM4A (9741)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_014713.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000059985 CCATTCTTCTGTTACCGACTT pLKO.1 439 CDS 100% 4.050 5.670 N LAPTM4A n/a
2 TRCN0000299332 CCATTCTTCTGTTACCGACTT pLKO_005 439 CDS 100% 4.050 5.670 N LAPTM4A n/a
3 TRCN0000059986 CGTCTGAGAGAATGGCTGATA pLKO.1 323 CDS 100% 4.950 3.960 N LAPTM4A n/a
4 TRCN0000059983 GCCGTCTCTGTTCTTATGTTT pLKO.1 361 CDS 100% 5.625 3.938 N LAPTM4A n/a
5 TRCN0000299330 GCCGTCTCTGTTCTTATGTTT pLKO_005 361 CDS 100% 5.625 3.938 N LAPTM4A n/a
6 TRCN0000059987 TCTCACCTATTTGCCAAGAAT pLKO.1 498 CDS 100% 5.625 3.938 N LAPTM4A n/a
7 TRCN0000299329 TCTCACCTATTTGCCAAGAAT pLKO_005 498 CDS 100% 5.625 3.938 N LAPTM4A n/a
8 TRCN0000059984 CCTGTTCATTGTTCTTGTGTT pLKO.1 591 CDS 100% 4.950 3.465 N LAPTM4A n/a
9 TRCN0000299255 CCTGTTCATTGTTCTTGTGTT pLKO_005 591 CDS 100% 4.950 3.465 N LAPTM4A n/a
10 TRCN0000110861 GCTGTCAACATTCAGTATGAA pLKO.1 283 CDS 100% 5.625 3.375 N Laptm4a n/a
11 TRCN0000325401 GCTGTCAACATTCAGTATGAA pLKO_005 283 CDS 100% 5.625 3.375 N Laptm4a n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_014713.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15676 pDONR223 0% 100% 100% None n/a
2 ccsbBroad304_15676 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000475138 AACGATTTTACCAGCGTGTTCTGC pLX_317 52.2% 100% 100% V5 n/a
4 ccsbBroadEn_07479 pDONR223 100% 99.7% 99.5% None 216G>T;301G>T n/a
5 ccsbBroad304_07479 pLX_304 0% 99.7% 99.5% V5 216G>T;301G>T n/a
6 TRCN0000466952 CGATTGCCGTTTTAGCATTAGGCG pLX_317 48.1% 99.7% 99.5% V5 216G>T;301G>T n/a
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