Construct: ORF TRCN0000475140

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010635.1_s317c1
Derived from:
ccsbBroadEn_06365
DNA Barcode:
CCCACAATGCGGTACGTGCAGGGA
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
HIF1A (3091)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000475140
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3091 HIF1A hypoxia inducible factor 1 ... NM_001530.4 99.9% 100% 12C>T
2 human 3091 HIF1A hypoxia inducible factor 1 ... NM_001243084.1 95.8% 96.1% (many diffs)
3 human 3091 HIF1A hypoxia inducible factor 1 ... NM_181054.3 88.9% 88.8% 12C>T;2202_2203insGGAAC;2205_2206ins268
4 mouse 15251 Hif1a hypoxia inducible factor 1,... XM_017314961.1 88.7% 90.5% (many diffs)
5 mouse 15251 Hif1a hypoxia inducible factor 1,... XM_006515478.3 88.6% 90.3% (many diffs)
6 mouse 15251 Hif1a hypoxia inducible factor 1,... NM_001313920.1 87.4% 89.3% (many diffs)
7 mouse 15251 Hif1a hypoxia inducible factor 1,... NM_010431.2 87.2% 89% (many diffs)
8 mouse 15251 Hif1a hypoxia inducible factor 1,... NM_001313919.1 87.1% 88.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2544
ORF length:
2478
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga gggcgctggc ggcgcgaacg acaagaaaaa gataagttct gaacgtcgaa
121aagaaaagtc tcgagatgca gccagatctc ggcgaagtaa agaatctgaa gttttttatg
181agcttgctca tcagttgcca cttccacata atgtgagttc gcatcttgat aaggcctctg
241tgatgaggct taccatcagc tatttgcgtg tgaggaaact tctggatgct ggtgatttgg
301atattgaaga tgacatgaaa gcacagatga attgctttta tttgaaagcc ttggatggtt
361ttgttatggt tctcacagat gatggtgaca tgatttacat ttctgataat gtgaacaaat
421acatgggatt aactcagttt gaactaactg gacacagtgt gtttgatttt actcatccat
481gtgaccatga ggaaatgaga gaaatgctta cacacagaaa tggccttgtg aaaaagggta
541aagaacaaaa cacacagcga agcttttttc tcagaatgaa gtgtacccta actagccgag
601gaagaactat gaacataaag tctgcaacat ggaaggtatt gcactgcaca ggccacattc
661acgtatatga taccaacagt aaccaacctc agtgtgggta taagaaacca cctatgacct
721gcttggtgct gatttgtgaa cccattcctc acccatcaaa tattgaaatt cctttagata
781gcaagacttt cctcagtcga cacagcctgg atatgaaatt ttcttattgt gatgaaagaa
841ttaccgaatt gatgggatat gagccagaag aacttttagg ccgctcaatt tatgaatatt
901atcatgcttt ggactctgat catctgacca aaactcatca tgatatgttt actaaaggac
961aagtcaccac aggacagtac aggatgcttg ccaaaagagg tggatatgtc tgggttgaaa
1021ctcaagcaac tgtcatatat aacaccaaga attctcaacc acagtgcatt gtatgtgtga
1081attacgttgt gagtggtatt attcagcacg acttgatttt ctcccttcaa caaacagaat
1141gtgtccttaa accggttgaa tcttcagata tgaaaatgac tcagctattc accaaagttg
1201aatcagaaga tacaagtagc ctctttgaca aacttaagaa ggaacctgat gctttaactt
1261tgctggcccc agccgctgga gacacaatca tatctttaga ttttggcagc aacgacacag
1321aaactgatga ccagcaactt gaggaagtac cattatataa tgatgtaatg ctcccctcac
1381ccaacgaaaa attacagaat ataaatttgg caatgtctcc attacccacc gctgaaacgc
1441caaagccact tcgaagtagt gctgaccctg cactcaatca agaagttgca ttaaaattag
1501aaccaaatcc agagtcactg gaactttctt ttaccatgcc ccagattcag gatcagacac
1561ctagtccttc cgatggaagc actagacaaa gttcacctga gcctaatagt cccagtgaat
1621attgttttta tgtggatagt gatatggtca atgaattcaa gttggaattg gtagaaaaac
1681tttttgctga agacacagaa gcaaagaacc cattttctac tcaggacaca gatttagact
1741tggagatgtt agctccctat atcccaatgg atgatgactt ccagttacgt tccttcgatc
1801agttgtcacc attagaaagc agttccgcaa gccctgaaag cgcaagtcct caaagcacag
1861ttacagtatt ccagcagact caaatacaag aacctactgc taatgccacc actaccactg
1921ccaccactga tgaattaaaa acagtgacaa aagaccgtat ggaagacatt aaaatattga
1981ttgcatctcc atctcctacc cacatacata aagaaactac tagtgccaca tcatcaccat
2041atagagatac tcaaagtcgg acagcctcac caaacagagc aggaaaagga gtcatagaac
2101agacagaaaa atctcatcca agaagcccta acgtgttatc tgtcgctttg agtcaaagaa
2161ctacagttcc tgaggaagaa ctaAATCCAA AGATACTAGC TTTGCAGAAT GCTCAGAGAA
2221AGCGAAAAAT GGAACATGAT GGTTCACTTT TTCAAGCAGT AGGAATTGGA ACATTATTAC
2281AGCAGCCAGA CGATCATGCA GCTACTACAT CACTTTCTTG GAAACGTGTA AAAGGATGCA
2341AATCTAGTGA ACAGAATGGA ATGGAGCAAA AGACAATTAT TTTAATACCC TCTGATTTAG
2401CATGTAGACT GCTGGGGCAA TCAATGGATG AAAGTGGATT ACCACAGCTG ACCAGTTATG
2461ATTGTGAAGT TAATGCTCCT ATACAAGGCA GCAGAAACCT ACTGCAGGGT GAAGAATTAC
2521TCAGAGCTTT GGATCAAGTT AACTGACCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA
2581AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC
2641TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC CCACAATGCG
2701GTACGTGCAG GGAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag
2761att

Download FASTA (ORF) (Full)

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